1. Neuritin promotes angiogenesis through inhibition of DLL4/Notch signaling pathway.
- Author
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Yang L, Wang X, Sun J, Liu C, Li G, Zhu J, and Huang J
- Subjects
- Adenocarcinoma of Lung blood supply, Adenocarcinoma of Lung pathology, Cell Movement genetics, Cell Proliferation genetics, Cells, Cultured, GPI-Linked Proteins genetics, GPI-Linked Proteins metabolism, Humans, Lung blood supply, Lung Neoplasms blood supply, Lung Neoplasms pathology, Neuropeptides genetics, Transfection, Up-Regulation genetics, Adaptor Proteins, Signal Transducing metabolism, Adenocarcinoma of Lung metabolism, Calcium-Binding Proteins metabolism, Human Umbilical Vein Endothelial Cells metabolism, Lung Neoplasms metabolism, Neovascularization, Pathologic metabolism, Neovascularization, Physiologic genetics, Neuropeptides metabolism, Receptor, Notch1 metabolism, Signal Transduction genetics
- Abstract
Neuritin is a member of the neurotrophic factor family, which plays an important role in the promotion and development of the nervous system. Neuritin is also involved in angiogenesis. Neuritin was recently found to be a negative regulatory factor of the Notch 1 signaling pathway. Notch signaling pathway is known as a regulatory pathway of angiogenesis. Thus, neuritin may play a role in angiogenesis through the Notch signaling pathway. In the present study, we investigated the expressions of neuritin and Notch signaling pathway factors in the pulmonary vascular tissue. The results showed that neuritin expression was increased in the paraneoplastic vascular tissue and decreased in the lung cancer vascular tissue. The neuritin expression was increased with the increase of vascular tissue density, and a negative correlation between neuritin expression and delta-like ligand 4 (DLL4) was identified in vascular tissues of lung cancer. Overexpression of neuritin in human umbilical vein endothelial cells (HUVECs) inhibited the expressions of Notch signaling pathway-associated factors, including DLL4, NICD, and Hes-1, and promoted the migration and tubular formation of HUVECs. In conclusion, our results indicated that neuritin is involved in angiogenesis and may play a role in angiogenesis through the Notch signaling pathway. This study provides a theoretical basis for clinical anti-angiogenesis therapy., (© The Author(s) 2021. Published by Oxford University Press on behalf of the Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
- Published
- 2021
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