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MYB is a common transcription factors widely involved in the regulation of anthocyanidin biosynthesis. In order to explore the regulatory role of MYB transcription factors in the biosynthesis of common buckwheat anthocyanidins, we screened and cloned a MYB gene associated with anthocyanin biosynthesis from the transcriptomic data of common buckwheat varieties of safflower common buckwheat and Beizaosheng, and named it FeR2R3-MYB, GenBank login number was MT151381.1. The sequence was analyzed by bioinformatics analysis and qRT-PCR was used to analyze the expression characteristics of FeR2R3-MYB gene in Beizaosheng and safflower common buckwheat. The results were as follows: (1) FeR2R3-MYB gene was 831 bp in total length, encoding 276 amino acids. The relative molecular mass of the protein was 30.95 kD, the theoretical isoelectric point (pI) was 8.73, and the instability index of the protein was 69.64, which belonged to the unstable protein. The total hydrophobic value was -0.679, and the whole peptide chain showed hydrophilic characteristics. (2) FeR2R3-MYB had a typical R2R3-MYB domain and belonged to the R2R3-MYB subfamily. (3) FeR2R3-MYB was closely related to common buckwheat and knotweed, belonging to the same family. (4) The promoter sequence of FeR2R3-MYB contained a total of nine light corresponding elements, 12 transcription factor binding sites, four abiotic corresponding elements and two hormone response elements. (5) Subcellular localization found that FeR2R3-MYB was only expressed in the nucleus. (6) The expression of FeR2R3-MYB gene of safflower common buckwheat was higher than that of Beizaosheng in leaves and inflorescences, and it was further speculated that FeR2R3-MYB gene could positively regulate the biosynthesis of common buckwheat anthocyanin. In summary, these results lay a foundation for further deepening the research on the function and expression regulation of FeR2R3-MYB gene in the biosynthetic pathway of common buckwheat anthocyanin.