1. Antigen association of J6-1 cell membrane associated factor receptor with macrophage colony stimulating factor receptor
- Author
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Qing Rao, Ke-Fu Wu, Jingshu Han, Guanjie Ma, Guoguang Zheng, Dexian Zheng, Yiqi Geng, and Shbuqing Luo
- Subjects
Macrophage colony-stimulating factor ,Cancer Research ,biology ,medicine.drug_class ,Chemistry ,Cell growth ,Cell ,Monoclonal antibody ,Molecular biology ,medicine.anatomical_structure ,Oncology ,Antigen ,Polyclonal antibodies ,medicine ,biology.protein ,Antibody ,Receptor - Abstract
Objective: To verify the antigen association of MAF-J6-1 receptor with M-CSFR and to further study the role of M-CSF and its receptor mediated juxtaerine in promoting leukemic cell proliferation. Methods: Monoclonal antibody (McAb) of MAF-J6-1R RE2 and polyclonal antibody (PolyAb) of rhM-CSFR were prepared. The specificity of MeAh RE2 to M-CSFR was confirmed by indirect ELISA, cross-neutralizing assay with J6-1 cell colony formation and neutralization test by ELISA. Results: The reactive activity of purified RE2 to M-CSFR was over 1: 16000. The inhibitory activity of M-CSFR and MAF-J6-1R could be blocked by RE2 and antI-M-CSFR antibody. The reactivity of RE2 to M-CSFR could be reduced by M-CSFR. Conclusion: The specificity of RE2 to M-CSFR was confirmed and the antigen association of MAF-J6-1R with M-CSFR was proved, It suggests that M-CSF and its receptor mediated auto-juxtaerine stimulation could be an operative mechanism in either leukemia or nonhematological malignancies.
- Published
- 1999
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