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Objective To analyze the clinical value of SPECT/CT in diagnosis of skull base bone invasion and clinical decision-making for nasopharyngeal carcinoma (NPC) ，and to compare their diagnostic value with SPECT/CT， CT，MRI，and MRI combined with SPECT (MRI-SPECT) for skull base bone invasion. Methods Before treatment，among 348 newly diagnosed NPC patients，CT scan was performed in 186 patients (group A) and the remaining 162 patients received MRI scan (group B) . Clinical doctors then made clinical management decisions according to the CT or MRI results. After that， all patients underwent 99Tcm-MDP SPECT/CT examination for nasopharyngeal local tomography，and the results were provided to the clinical doctors to make clinical management decisions again. The changes between the two clinical management decisions were scored according to diagnosis， range of lesion， staging， treatment regimens， and auxiliary examination. The diagnostic value of CT scan，MRI scan，SPECT/CT and MRI-SPECT for skull base bone invasion was then evaluated and compared. Results In terms of changes in scores of clinical management decisions， the score of group A was 1.387 and group B was 0.951，showing a significant difference between the two groups by Wilcoxon test (Z = 6.570，P<0.001). By X2 test，there were correlations between CT and SPECT/CT (X2 = 98.495 ,P<0.001) , and between MRI and SPECT/CT (X2 = 32.662, P < 0.001). The consistency of CT and SPECT/CT (Kappa = 0.713) was greater than MRI and SPECT (Kappa = 0.449). The sensitivity of CT, MRI, SPECT/CT and MRI-SPECT was 67.1%, 84.5%, 90.8% and 100%, the specificity was 73.3%, 92.3%, 85.6% and 84.6%, and the area under the ROC curve was 0.702, 0. 884, 0. 882 and 0. 923, respectively. Conclusions SPECT/CT has important impact on clinical management decision for NPC. In the judgement of skull base invasion, the diagnostic value of SPECT/CT is significantly higher than CT and approximately equal to MRI. SPECT/CT should be one of the routine examination methods of nasopharyngeal carcinoma. In addition, in view of its greater diagnostic value, MRI combined with SPECT should be the focus of future imaging studies. [ABSTRACT FROM AUTHOR]

Objective To inquire into the influence of silencing HMGB1 expression by small interfering RNA (siRNA) on cell growth，proliferation, invasion and metastasis of colorectal cancer LoVo cells both in vitro and in vivo. Methods Lentivirus-mediated HMGB1 siRNA was transfected into LoVo cells to silence the HMGB1 expression. The HMGB1 mRNA and protein expression after siRNA transfection was detected by RT-PCR and Western blot. MTT assay was used to observe the cell proliferation and to draw a growth curve. Cell cycle was measured by flow，cytometry. The ability of invasion and speed of cell migration were evaluated by transwell chamber invasion and cell scratch assay. The influence of HMGB1 silencing on the proliferation of LoVo cells in vivo was observed in LoVo tumor-bearing nude mice. Results Lentivirus- mediated siRNA was successfully transfected into colorectal cancer cell line LoVo. The expression of HMGB1 mRNA and protein in the HMGBl-siRNA group wee 0.24±0.04 and 0.21 ±0.03，respectively. Compared with the HMGB1-siRNA-Neg group (0.82±0.13，1.15±0.18) and control group (0.93±0.15, 1.21 ±0.20), the difference was significant ( P<0.05). MTT assay show-ed that the cell proliferation in the HMGBl-siRNA group was significantly inhibited when compared with that in the HMGB1-siRNA-Neg group and control group (P<0.05). Flow，cytometry showed that the proliferation index (PI) of HMGB1-siRNA group w，as 38.27± 1.32，significantly lower than 54.66± 1.74 in the HMGB1-siRNA-Neg group and 57.43± 1.29 in the control group (P <0.05). The transwell assay showed that the number of penetrated cells in the HMGB1-siRNA group w，as 14.0±3.5，significantly low，er than 51.0±6.7 in the HMGB1-siRNA-Neg group and 68.0±5.3 in the control group (P<0.05). Similarly， the scrape wound recovered significantly slower in the HMGB1- siRNA group (83.61±23.21) pm than that in the other tww groups (202.86±46.46) pm and (214.58±57.38) pm (P<0.05). The nude mouse xenograft tumor experiment show，ed that the final tumor volume w，as (521 ±34) mm3 in the HMGB1-siRNA group，significantly smaller than that in the HMGB1-siRNA-Neg group of (763±46) mm3 and control group of ( 802 ± 51) mm3 ( P < 0.05 ). Conclusions Lentivirus-mediated HMGBl- siRNA can effectively inhibit the HMGB1 expression in colorectal cancer LoVo cells both in vitro and in vivo. HMGB1 gene silencing can slow the growth of colorectal cancer cells, extend the cell proliferation cycle, decrease their invasion and migration, and significantly inhibit the growth of xenograft tumor in nude mice. [ABSTRACT FROM AUTHOR]