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Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Anterior subtotal corpectomy, decompression and fusion is a conventional method to treat cervical degenerative diseases. A titanium cage is an important implant to maintain the stability of the cervical spine after subtotal corpectomy. In recent years, many patients have complications such as titanium cage sinking, which are highly controversial. OBJECTIVE: To investigate the internal biomechanical relationship between the tilt angle of the titanium cage and postoperative titanium cage subsidence after anterior subtotal cervical corpectomy, decompression and fusion. METHODS: A three-dimensional finite element model of the C4-C6 segment was established by CT images of a normal human cervical spine, in which the anterior subtotal resection, decompression and fusion of the C5 vertebral body were simulated, and titanium cages with different tilt angles (-6° to -1° negative angle, that is, the front edge of titanium cage is shorter than the rear edge of titanium cage; 1° to 6° positive angle, that is, the front edge of titanium cage is longer than the rear edge of titanium cage) were placed. After setting the boundary conditions, preloads of 50, 100 and 150 N were applied respectively on the C4 vertebral body. The stress value of each contact point between the titanium cage and C4 lower-end plate and C6 lower-end plate (seven stress contact points on the contact surface of titanium mesh) was recorded and statistical analysis was conducted. RESULTS AND CONCLUSION: (1) The tilt angles of the titanium cage of the positive angle group and negative angle group under 50, 100 and 150 N stress respectively were found by Mann Whitn test, with P < 0.05, which was statistically significant. The dispersion coefficients of the positive angle group were smaller than those of the negative angle group under 50, 100 and 150 N stress conditions. (2) Under 50, 100 and 150 N stress conditions, the Wilcoxon sign rank test in the positive angle group of titanium cage tilt angle found that when the angle was set to 1° to 5°, the difference was not statistically significant (P > 0.05). However, when the tilt angle of the titanium cage was set to 6°, the difference was statistically significant (P < 0.05). (3) Under 50, 100 and 150 N stress conditions, the Wilcoxon sign rank test in the negative angle group of titanium cage tilt angle found that when the tilt angle was set to -1° to-6°, the difference was not statistically significant (P > 0.05). (4) It is concluded that in the sagittal position, the titanium cage with a positive tilt angle is more stable than with a negative tilt angle, which is more suitable for clinical use. The tilt angle of the titanium cage is relatively stable in the range of 1° to 5°. When the tilt angle is 6°, the stability starts to decline, which is easy to cause complications of titanium cage sinking after surgery. It is more suitable to select the titanium cage with a tilt angle of 1° to 5° according to the clinical situation during surgery to improve the efficacy. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Percutaneous endoscopic interbody foraminal interbody fusion is an advanced minimally invasive method for the treatment of lumbar degenerative diseases. The traditional interbody fusion cage is not fully suitable for minimally invasive surgery. The choice of fusion cage for endoscopic lumbar fusion is still controversial. OBJECTIVE: To explore the clinical effect of percutaneous spinal endoscopic implantation of height adjustable titanium fusion cage for interbody fusion in the treatment of lumbar degenerative diseases. METHODS: A retrospective study investigated 55 patients who underwent percutaneous endoscopic lumbar fusion in the Department of Orthopedics of First Affiliated Hospital of Guangxi University of Chinese Medicine from December 2018 to June 2019. According to the different types of fusion cages implanted, they were divided into two groups. The stretch group (n=23) was implanted with a retractable height adjustable titanium fusion cage, and the general group (n=32) was used with an ordinary lumbar fusion cage. Postoperative complications were recorded in the two groups. The JOA score, Visual Analogue Scale score, and Oswestry Disability Index in the two groups were compared before and after treatment. This study was approved by the Ethics Committee of the First Affiliated Hospital of Guangxi University of Chinese Medicine. RESULTS AND CONCLUSION: (1) All 55 patients were followed-up for more than 12 months. All patients obtained bony fusion without complications, such as internal fixation loosening, fracture, fusion cage displacement, or settlement. (2) Compared with those before treatment, Visual Analogue Scale scores of low back pain and leg pain, JOA score, and Oswestry Disability Index were significantly improved in the two groups at 2 weeks, 3, 6, and 12 months after operation (P < 0.05). JOA score at 12 months and Oswestry Disability Index at 6 and 12 months were significantly better in the stretch group than those in the general group (P < 0.05). (3) Compared with those before treatment, the height of the intervertebral space, the height of the intervertebral foramen of the fusion segment, the lordosis angle of the operative segment, and the lumbar lordosis angle of the two groups were significantly improved after treatment (P < 0.05). The height of the intervertebral space, the height of the intervertebral foramen of the fusion segment, the lordosis angle of the operative segment, and the lumbar lordosis angle were better in the stretch group than those of the general group at 5 days and 12 months after treatment (P < 0.05). (4) It is concluded that percutaneous endoscopic lumbar fusion is a reliable minimally invasive method for the treatment of lumbar degenerative diseases. Its combination with highly adjustable titanium fusion cage can improve its clinical efficacy. [ABSTRACT FROM AUTHOR]

OBJECTIVE: The up-to-now researches on the effect of transcranial direct current stimulation (tDCS) on exercise performance are in the initial phase, and the existing conclusions are inconsistent. This meta-analysis attempted to quantitatively evaluate the effect of tDCS on muscle strength and endurance performance of healthy adults, providing new evidence for new neuroelectrophysiological techniques as well as improving the muscle function. METHODS: To collect randomized controlled trial (RCT) studies regarding the effects of tDCS on muscle strength and endurance performance in healthy adults, the following databases were searched from inception to October 2019: Web of Science, PubMed, Sport Discus, and CNKI. According to the Cochrane Systematic Review Manual, Review Manager 5.3 software was used to evaluate the methodological quality of the literature included and to analyze the combined effect amount. RESULTS: A total of 15 RCTs involving 201 healthy subjects were included. The meta-analysis indicated that compared with the experimental group, tDCS intervention had insufficient evidence in improving muscle strength: I²=47%, 95% confidence interval [-0.79, 0.23], P=0.27 > 0.05, standard mean difference=-0.28. Compared with the control group, the function of tDCS stimulation to improve the performance of endurance was more evident, and the combined effect amount tended to a medium effect: I²=57%, 95% confidence interval [0.10, 0.76], P=0.01 < 0.05, and standard mean difference=0.43. CONCLUSION: tDCS intervention has no statistical significance in improving strength, but it is effective in improving endurance performance in general. More rigorous and extensive experimental researches are warranted to explore the tDCS neurophysiological mechanism. [ABSTRACT FROM AUTHOR]

BACKGROUND: Development of bone tissue engineering provides a new method to solve bone defect repair. Scaffold material and structure construction are issues of concern. OBJECTIVE: To fabricate a silk fibroin and hydroxyapatite scaffold with biomimetic interconnected macropores structure using the paraffin-sphere leaching technology and to evaluate its possibility of cytocompatibility. METHODS: The scaffold with biomimetic interconnected macropores structure was made by the paraffin-sphere leaching technology. The structure of scaffold was observed by the stereomicroscope and scanning electron microscope. The pore size and elasticity modulus were calculated. Passage 3 rabbit adipose-derived mesenchymal stem cells were seeded into the scaffold. The cell viability was detected by live/dead staining at 48 hours after culture. The cell adhesion was observed by hematoxylin-eosin staining at 1 week of culture. The scaffold-cell composite was observed under scanning electron microscope at 3 days of culture. The cell proliferation was detected by the cell counting-kit 8 assay at 1, 3, 5 and 7 days of culture. Those cells cultured alone were considered as control group. RESULTS AND CONCLUSION: (1) Stereomicroscope showed the ivory silk fibroin/hydroxyapatite scaffold. Scanning electron microscope revealed pore structures in cross-section and longitudinal-section with good connectivity. The scaffold pore size was (362.23±26.52) μm and the elasticity modulus was (54.93±5.44) kPa. (2) Scanning electron microscope showed that adipose-derived mesenchymal stem cells adhered and stretched well in the pore wall and connected pore, secreted abundant extracellular matrix, and filled in the pores of silk fibroin/hydroxyapatite scaffold. (3) Hematoxylin-eosin staining results found that adipose-derived mesenchymal stem cells evenly adhered onto the inner wall of silk fibroin/hydroxyapatite scaffold, and proliferated well. (4) Live/dead staining revealed a good viability of adipose-derived mesenchymal stem cells. (5) Cell counting-kit 8 assay results showed the good proliferation of adipose-derived mesenchymal stem cells on the scaffold. (6) To conclude, the silk fibroin/hydroxyapatite scaffold possesses good pore size and cytocompatibility. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)