1. Effects of human amniotic fluid on cartilage regeneration from free perichondrial grafts in rabbits
- Author
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Mesut Özcan, Gülaydan Filiz, Güzin Yeşim Özgenel, Uludağ Üniversitesi/Tıp Fakültesi/Plastik ve Rekonstrüktif Cerrahi Anabilim Dalı., Özgenel, Güzin Yeşim, Filiz, Gülaydan, Özcan, Mesut, and AAH-4233-2021
- Subjects
Suturing method ,Pathology ,Amniotic fluid ,Back muscle ,medicine.medical_treatment ,Sodium hyaluronate injections ,Rabbit ,Randomization ,Animal tissue ,Extracellular matrix ,chemistry.chemical_compound ,Fibroblast-growth-factor ,Cartilage transplantation ,Perichondrium ,Hyaluronic acid ,Analysis of variance ,Saline ,Priority journal ,Anatomy ,Growth factor ,Statistical significance ,Amnion fluid ,medicine.anatomical_structure ,Tissue graft ,Defects ,Rabbits ,Nuki experimental-model ,Growth factors ,Thickness ,Chondrogenesis ,Human amniotic fluid ,Human ,medicine.medical_specialty ,Chondroitin sulfate ,Histology ,Sodium chloride ,Neochondrogenesis ,Article ,Dogs ,medicine ,Postoperative period ,Animals ,Humans ,Regeneration ,Animal model ,Animal experiment ,Osteo-arthritis ,business.industry ,Articular-cartilage ,Cartilage ,Wound Healing ,Cicatrix ,Fibroblasts ,Ear cartilage ,Nonhuman ,Otorhinolaryngology ,chemistry ,Tissue regeneration ,Surgery ,business ,Controlled study ,Repair - Abstract
After the chondrogenic potential of free grafts of perichondrium was shown in several experimental studies, perichondrium has been used to reconstruct cartilage tissue in various clinical situations. This study investigates the effects of human amniotic fluid on neochondrogenesis from free perichondrial grafts in a rabbit model. Since this fluid contains high concentrations of hyaluronic acid, hyaluronic acid-stimulating activator, growth factors, and extracellular matrix precursors during the second trimester, it may have a stimutating effect on neochondrogenesis. Perichondrial grafts, measuring 20 x 20 mm(2) were obtained from the ears of 144 New Zealand young rabbits and were sutured over the paravertebral muscles. The rabbits were randomly divided into three groups with 48 rabbits per group. In group 1, 0.3 ml human amniotic fluid, and in group 2, 0.3 ml saline were injected underneath the perichondrial grafts. Group 3 formed the control group in which no treatment was given. Histologically, neochondrogenesis was evaluated in terms of cellular form and graft thickness at 2, 4, 6, and 8 weeks after surgery. In group 1, the mature cartilage was generated quickly and the cartilage plate in this group was significantly thick and extensive when compared with groups 2 and 3 at 8 weeks (p < 0.05, ANOVA). In conclusion, our study shows that human amniotic fluid enhances neochondrogenesis from free perichondrial grafts. The rich content of hyaluronic acid and growth factors possibly participate in this result. Bursa Plastik Cerrahi-Yanık ve El Cerrahisi Derneği
- Published
- 2004