1. Gene expression profile in TNF receptor-associated periodic syndrome reveals constitutively enhanced pathways and new players in the underlying inflammation.
- Author
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Borghini S, Ferrera D, Prigione I, Fiore M, Ferraris C, Mirisola V, Amaro AA, Gueli I, Zammataro L, Gattorno M, Pfeffer U, and Ceccherini I
- Subjects
- Adolescent, Case-Control Studies, Child, Child, Preschool, Female, Fever diagnosis, Fever immunology, Fever metabolism, Gene Expression Profiling methods, Genetic Association Studies, Genetic Markers, Genetic Predisposition to Disease, Hereditary Autoinflammatory Diseases diagnosis, Hereditary Autoinflammatory Diseases immunology, Hereditary Autoinflammatory Diseases metabolism, Heterozygote, Humans, Inflammation Mediators immunology, Interleukin 1 Receptor Antagonist Protein genetics, Interleukin 1 Receptor Antagonist Protein metabolism, Lipopolysaccharides pharmacology, Male, Monocytes drug effects, Monocytes immunology, Mutation, Oligonucleotide Array Sequence Analysis, Phenotype, Polymerase Chain Reaction, Receptors, Tumor Necrosis Factor, Type I genetics, Receptors, Tumor Necrosis Factor, Type I immunology, Reproducibility of Results, Fever genetics, Gene Expression Regulation drug effects, Hereditary Autoinflammatory Diseases genetics, Inflammation Mediators metabolism, Monocytes metabolism
- Abstract
Objectives: Tumour necrosis factor (TNF) receptor-associated periodic syndrome (TRAPS) is a multisystemic autoinflammatory condition associated with heterozygous TNFRSF1A mutations, presenting with a variety of clinical symptoms, many of which yet unexplained. In this work, we aimed at deepening into TRAPS pathogenic mechanisms sustained by monocytes., Methods: Microarray experiments were conducted to identify genes whose expression results altered in patients compared to healthy individuals, both under basal condition and following LPS stimulation., Results: An inflammatory state baseline, characterised by constitutive overexpression of IL1β and IL1R1 receptor, has been shown in TRAPS patients compared to controls, including in non-active disease phases. Following LPS stimulation, IL1RN up-regulation is stronger in controls than in patients and inflammatory pathways and microRNAs undergo differential regulation. Genes involved in post-translational modifications, protein folding and ubiquitination result constitutively up-regulated in TRAPS, while response to interferon types I and II is defective, failing to be up-regulated by LPS. TGFβ pathway is down-regulated in untreated TRAPS monocytes, while genes involved in redox regulation result constitutively over-expressed. Finally, additional molecular alterations seem to reflect organ failures sometime complicating the disease., Conclusions: Gene expression profile in resting TRAPS monocytes has confirmed the patients' chronic inflammatory condition. In addition, pathways not yet associated with the disease have been disclosed, such as interferon types I and II response to LPS stimulation and a downregulation of the TGFβ pathway in basal condition. The role of miRNA, suggested by our results, deserves in-depth analyses in light of the possible development of targeted therapies.
- Published
- 2016