10 results on '"Barba-Spaeth, Giovanna"'
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2. Omicron BA.1 breakthrough infection drives long-term remodeling of the memory B cell repertoire in vaccinated individuals
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Sokal, Aurélien, primary, Barba-Spaeth, Giovanna, additional, Hunault, Lise, additional, Fernández, Ignacio, additional, Broketa, Matteo, additional, Meola, Annalisa, additional, Fourati, Slim, additional, Azzaoui, Imane, additional, Vandenberghe, Alexis, additional, Lagouge-Roussey, Pauline, additional, Broutin, Manon, additional, Roeser, Anais, additional, Bouvier-Alias, Magali, additional, Crickx, Etienne, additional, Languille, Laetitia, additional, Fournier, Morgane, additional, Michel, Marc, additional, Godeau, Bertrand, additional, Gallien, Sébastien, additional, Melica, Giovanna, additional, Nguyen, Yann, additional, Canoui-Poitrine, Florence, additional, Noizat-Pirenne, France, additional, Megret, Jérôme, additional, Pawlotsky, Jean-Michel, additional, Fillatreau, Simon, additional, Reynaud, Claude-Agnès, additional, Weill, Jean-Claude, additional, Rey, Félix A., additional, Bruhns, Pierre, additional, Mahévas, Matthieu, additional, and Chappert, Pascal, additional
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- 2023
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3. Environmental and genetic drivers of population differences in SARS-CoV-2 immune responses
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Aquino, Yann, primary, Bisiaux, Aurélie, additional, Li, Zhi, additional, O’Neill, Mary, additional, Mendoza-Revilla, Javier, additional, Merkling, Sarah Hélène, additional, Kerner, Gaspard, additional, Hasan, Milena, additional, Libri, Valentina, additional, Bondet, Vincent, additional, Smith, Nikaïa, additional, de Cevins, Camille, additional, Ménager, Mickaël, additional, Luca, Francesca, additional, Pique-Regi, Roger, additional, Barba-Spaeth, Giovanna, additional, Pietropaoli, Stefano, additional, Schwartz, Olivier, additional, Leroux-Roels, Geert, additional, Lee, Cheuk-Kwong, additional, Leung, Kathy, additional, Wu, Joseph T.K., additional, Peiris, Malik, additional, Bruzzone, Roberto, additional, Abel, Laurent, additional, Casanova, Jean-Laurent, additional, Valkenburg, Sophie A., additional, Duffy, Darragh, additional, Patin, Etienne, additional, Rotival, Maxime, additional, and Quintana-Murci, Lluis, additional
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- 2022
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4. Omicron BA.1 breakthrough infection drives long-term remodeling of the memory B cell repertoire in vaccinated individuals
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Sokal, Aurélien, Barba-Spaeth, Giovanna, Hunault, Lise, Fernández, Ignacio, Broketa, Matteo, Meola, Annalisa, Fourati, Slim, Azzaoui, Imane, Vandenberghe, Alexis, Lagouge-Roussey, Pauline, Broutin, Manon, Roeser, Anais, Bouvier-Alias, Magali, Crickx, Etienne, Languille, Laetitia, Fournier, Morgane, Michel, Marc, Godeau, Bertrand, Gallien, Sébastien, Melica, Giovanna, Nguyen, Yann, Canoui-Poitrine, Florence, Noizat-Pirenne, France, Megret, Jérôme, Pawlotsky, Jean-Michel, Fillatreau, Simon, Reynaud, Claude-Agnès, Weill, Jean-Claude, Rey, Félix, Bruhns, Pierre, Mahévas, Matthieu, Chappert, Pascal, Institut Necker Enfants-Malades (INEM - UM 111 (UMR 8253 / U1151)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Hôpital Beaujon [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), CIC - CHU Henri Mondor, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Virologie Structurale - Structural Virology, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Anticorps en thérapie et pathologie - Antibodies in Therapy and Pathology, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Physiologie, physiopathologie et thérapeutique (ED 394), Sorbonne Université (SU), Centre d'Immunologie et des Maladies Infectieuses (CIMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), CHU Henri Mondor [Créteil], Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Hôpital Henri Mondor, Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Hôpital Cochin [AP-HP], IMRB - CEPIA/'Clinical Epidemiology And Ageing : Geriatrics, Primary Care and Public Health' [Créteil] (U955 Inserm - UPEC), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Etablissement Français du Sang [Créteil], Structure Fédérative de Recherche Necker (SFR Necker - UMS 3633 / US24), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), and This work was funded by the CAPNET (Comité ad-hoc de pilotage national des essaisthérapeutiques et autres recherches, French government) and by the Fondation PrincesseGrace. Assistance Publique – Hôpitaux de Paris (AP-HP, Département de la Recherche Cli-nique et du Développement) was the promotor and sponsor of MEMO-COV-2. Work in theUnit of Structural Virology was funded by Institut Pasteur, Urgence COVID-19 FundraisingCampaign of Institut Pasteur. PB acknowledges funding from the Institut Pasteur and InsitutNational de la Santé et de la Recherche Médicale (INSERM). AS was supported by a Posted’accueil from INSERM.
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[SDV]Life Sciences [q-bio] ,[SDV.IMM]Life Sciences [q-bio]/Immunology - Abstract
SummaryHow infection by a viral variant showing antigenic drift impacts a preformed mature human memory B cell (MBC) repertoire remains an open question. Here, we studied the MBC response up to 6 months after Omicron BA.1 breakthrough infection in individuals previously vaccinated with three doses of mRNA vaccine. Longitudinal analysis, using single-cell multi-omics and functional analysis of monoclonal antibodies from RBD-specific MBCs, revealed that a BA.1 breakthrough infection mostly recruited pre-existing cross-reactive MBCs with limitedde novoresponse against BA.1-restricted epitopes. Reorganization of clonal hierarchy and new rounds of germinal center reaction, however, combined to maintain diversity and induce progressive maturation of the MBC repertoire against common Hu-1 and BA.1, but not BA.5-restricted, SARS-CoV-2 Spike RBD epitopes. Such remodeling was further associated with marked improvement in overall neutralizing breadth and potency. These findings have fundamental implications for the design of future vaccination booster strategies.
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- 2023
5. Molecular mechanisms regulating the pH-dependent pr/E interaction in yellow fever virus
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Crampon, E., Covernton, E., Vaney, M.C., Dellarole, M., Sharma, A., Haouz, A., England, P., Lepault, J., Duquerroy, S., Rey, F.A., Barba-Spaeth, Giovanna, Virologie Structurale - Structural Virology, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Cristallographie (Plateforme) - Crystallography (Platform), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Biophysique Moléculaire (plateforme) - Molecular Biophysics (platform), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP), This work was supported by the French ANR (Agence Nationale de la Recherche), grants ANR-17-CE15-0031-01 FLAVIMMUNITY to GBS., We thank the staff at beamlines PX1 and PX2 at Soleil synchrotron (St. Aubin, France) and at PX beamlines at the ESRF (Grenoble, France), Franz X. Heinz and Karin Stiasny from Center for Virology, Medical University of Vienna, Austria for the gift of DENV pr protein and the plasmid for the production of ZIKV PF13 sE protein, Pablo Guardado-Calvo and Ignacio Fernandez from the Rey lab for helping with the MALS experiments and Alexander Rouvinski for help with the setting of the fusion experiments., and ANR-17-CE15-0031,FLAVIMMUNITY,Comprendre les mécanismes de l'efficacité du vaccin de la fièvre jaune 17D: Une approche immuno-structurelle vers la conception d'un vaccin Pan-flavivirus(2017)
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[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM] ,[SDV]Life Sciences [q-bio] - Abstract
Flavivirus particles bud in the ER of infected cells as immature virions composed of 180 heterodimers of glycoproteins prM and E, associated as 60 (prM/E)3trimeric spikes. Exposure to the mildly acidic pH of the TGN results in dissociation of the trimeric spikes followed by reassociation of the prM/E protomers into 90 dimers organized in a characteristic herringbone pattern. The furin site in prM is exposed in the dimers for maturation of prM into M and pr. For flaviviruses such as the tick-borne encephalitis virus (TBEV) as well as for dengue virus, it was shown that at neutral pH pr loses affinity for E, such that it dissociates from the mature particle as soon as it reaches the external milieu, which is at neutral pH. Using a soluble recombinant form of E (sE) and pr from yellow fever virus (YFV), we show here that the affinity of pr for recombinant E protein remains high even at neutral pH. The X-ray structure of YFV pr/sE shows more extensive inter-chain hydrogen bonding than does the dengue or TBEV, and also that it retains the charge complementarity between the interacting surfaces of the two proteins even at neutral pH. We further show that pr blocks sE flotation with liposomes when exposed at low pH at a 1:1 stoichiometry, yet in the context of the virus particle, an excess of 10:1 pr:E ratio is required to block virus/liposome fusion. In aggregate, our results show that the paradigm obtained from earlier studies of other flaviviruses does not apply to yellow fever virus, the flavivirus type species. A mechanism that does not rely solely in a change in the environmental pH is thus required for the release of pr from the mature particles upon release from infected cells. These results open up new avenues to understand the activation mechanism that yields mature, infectious YFV particles.
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- 2022
6. Immune escape of SARS-CoV-2 Omicron variant from mRNA vaccination-elicited RBD-specific memory B cells.
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Sokal, Aurelien, primary, Broketa, Matteo, additional, Meola, Annalisa, additional, Barba-Spaeth, Giovanna, additional, Fernandez, Ignacio, additional, Fourati, Slim, additional, Azzaoui, Imane, additional, de La Selle, Andrea, additional, Vandenberghe, Alexis, additional, Roeser, Anais, additional, Bouvier-Alias, Magali, additional, Crickx, Etienne, additional, Languille, Laetitia, additional, Michel, Marc, additional, Godeau, Bertrand, additional, Gallien, Sebastien, additional, Melica, Giovanna, additional, Nguyen, Yann, additional, Zarrouk, Virginie, additional, Canoui-Poitrine, Florence, additional, Noizat-Pirenne, France, additional, Megret, Jerome, additional, Pawlotsky, Jean-Michel, additional, Fillatreau, Simon, additional, Simon-Loriere, Etienne, additional, Weill, Jean-Claude, additional, Reynaud, Claude-Agnes, additional, Rey, Felix A., additional, Bruhns, Pierre, additional, Chappert, Pascal, additional, and Mahevas, Matthieu, additional
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- 2021
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7. Tunneling nanotubes provide a novel route for SARS-CoV-2 spreading between permissive cells and to non-permissive neuronal cells
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Pepe, Anna, primary, Pietropaoli, Stefano, additional, Vos, Matthijn, additional, Barba-Spaeth, Giovanna, additional, and Zurzolo, Chiara, additional
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- 2021
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8. Memory B cells control SARS-CoV-2 variants upon mRNA vaccination of naive and COVID-19 recovered individuals
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Sokal, Aurélien, primary, Barba-Spaeth, Giovanna, additional, Fernández, Ignacio, additional, Broketa, Matteo, additional, Azzaoui, Imane, additional, La Selle, Andrea de, additional, Vandenberghe, Alexis, additional, Fourati, Slim, additional, Roeser, Anais, additional, Meola, Annalisa, additional, Bouvier-Alias, Magali, additional, Crickx, Etienne, additional, Languille, Laetitia, additional, Michel, Marc, additional, Godeau, Bertrand, additional, Gallien, Sébastien, additional, Melica, Giovanna, additional, Nguyen, Yann, additional, Zarrouk, Virginie, additional, Canoui-Poitrine, Florence, additional, Noizat-Pirenne, France, additional, Megret, Jérôme, additional, Pawlotsky, Jean-Michel, additional, Fillatreau, Simon, additional, Bruhns, Pierre, additional, Rey, Felix A., additional, Weill, Jean-Claude, additional, Reynaud, Claude-Agnès, additional, Chappert, Pascal, additional, and Mahévas, Matthieu, additional
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- 2021
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9. Sensitive visualization of SARS-CoV-2 RNA with CoronaFISH
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Rensen, Elena, primary, Pietropaoli, Stefano, additional, Mueller, Florian, additional, Weber, Christian, additional, Souquere, Sylvie, additional, Isnard, Pierre, additional, Rabant, Marion, additional, Gibier, Jean-Baptiste, additional, Simon-Loriere, Etienne, additional, Rameix-Welti, Marie-Anne, additional, Pierron, Gérard, additional, Barba-Spaeth, Giovanna, additional, and Zimmer, Christophe, additional
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- 2021
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10. Maturation and persistence of the anti-SARS-CoV-2 memory B cell response
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Sokal, Aurélien, primary, Chappert, Pascal, additional, Roeser, Anais, additional, Barba-Spaeth, Giovanna, additional, Fourati, Slim, additional, Azzaoui, Imane, additional, Vandenberghe, Alexis, additional, Fernandez, Ignacio, additional, Bouvier-Alias, Magali, additional, Crickx, Etienne, additional, Ferchiou, Asma Beldi, additional, Hue, Sophie, additional, Languille, Laetitia, additional, Baloul, Samia, additional, Noizat-Pirenne, France, additional, Luka, Marine, additional, Megret, Jérôme, additional, Ménager, Mickaël, additional, Pawlotsky, Jean-Michel, additional, Fillatreau, Simon, additional, Rey, Felix A, additional, Weill, Jean-Claude, additional, Reynaud, Claude-Agnès, additional, and Mahévas, Matthieu, additional
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- 2020
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