1. Analyzing the Colocalization of MAP1LC3 and Lysosomal Markers in Primary Cells
- Author
-
Kanchan Phadwal
- Subjects
medicine.diagnostic_test ,Microtubule-associated protein ,Optical Imaging ,Autophagy ,Colocalization ,Cell sorting ,Biology ,Flow Cytometry ,Immunoglobulin light chain ,Molecular biology ,General Biochemistry, Genetics and Molecular Biology ,Cell biology ,Flow cytometry ,medicine.anatomical_structure ,medicine ,Animals ,Humans ,Bone marrow ,Primary cell ,Lysosomes ,Microtubule-Associated Proteins ,Biomarkers - Abstract
This technique evaluates the colocalization of the autophagy protein MAP1LC3 (microtubule-associated proteins 1A/1B light chain 3B, here referred to as LC3) with lysosomes (autolysosomes) in primary cells in a high-throughput manner. It uses an imaging fluorescence-activated cell sorting cytometer called the ImageStream to concomitantly detect surface molecules, making possible the identification of cells in mixed cell populations (e.g., in blood or bone marrow). It can be applied to clinical samples and to rare cell populations because only a few cells are needed for detection.
- Published
- 2015
- Full Text
- View/download PDF