Search

Your search keyword '"Kuznetsova, Elizaveta"' showing total 4 results
Start Over Author "Kuznetsova, Elizaveta" Publisher cold spring harbor laboratory
Sorry, I don't understand your search. ×
4 results on '"Kuznetsova, Elizaveta"'

1. Two distinct mechanisms of flavoprotein spectral tuning revealed by low-temperature and time-dependent spectroscopy

Author

Nikolaev, Andrey, primary, Tropina, Elena V., additional, Boldyrev, Kirill N., additional, Maksimov, Eugene G., additional, Borshchevskiy, Valentin, additional, Mishin, Alexey, additional, Yudenko, Anna, additional, Kuzmin, Alexander, additional, Kuznetsova, Elizaveta, additional, Semenov, Oleg, additional, Remeeva, Alina, additional, and Gushchin, Ivan, additional

Published
2023
Full Text
View/download PDF

2. luxAgene fromEnhygromyxa salinaencodes a functional homodimeric luciferase

Author

Yudenko, Anna, primary, Bazhenov, Sergey V., additional, Aleksenko, Vladimir A., additional, Goncharov, Ivan M., additional, Semenov, Oleg, additional, Remeeva, Alina, additional, Nazarenko, Vera V., additional, Kuznetsova, Elizaveta, additional, Fomin, Vadim V., additional, Konopleva, Maria N., additional, Al Ebrahim, Rahaf, additional, Sluchanko, Nikolai N., additional, Ryzhykau, Yury, additional, Semenov, Yury S., additional, Kuklin, Alexander, additional, Manukhov, Ilya V., additional, and Gushchin, Ivan, additional

Published
2023
Full Text
View/download PDF

3. Fine spectral tuning of a flavin-binding fluorescent protein for multicolor imaging

Author

Nikolaev, Andrey, primary, Yudenko, Anna, additional, Smolentseva, Anastasia, additional, Bogorodskiy, Andrey, additional, Tsybrov, Fedor, additional, Borshchevskiy, Valentin, additional, Bukhalovich, Siarhei, additional, Nazarenko, Vera V., additional, Kuznetsova, Elizaveta, additional, Semenov, Oleg, additional, Remeeva, Alina, additional, and Gushchin, Ivan, additional

Published
2022
Full Text
View/download PDF

4. Fine spectral tuning of a flavin-binding fluorescent protein for multicolor imaging

Author

Andrey Nikolaev, Anna Yudenko, Anastasia Smolentseva, Andrey Bogorodskiy, Fedor Tsybrov, Valentin Borshchevskiy, Siarhei Bukhalovich, Vera V. Nazarenko, Elizaveta Kuznetsova, Oleg Semenov, Alina Remeeva, Ivan Gushchin, Semenov, Oleg/0000-0001-9470-0013, Nikolaev, Andrey/0009-0004-2479-5124, Borshchevskiy, Valentin/0000-0003-4398-9712, Nikolaev, Andrey, Yudenko, Anna, SMOLENTSEVA, Anastasiia, Bogorodskiy, Andrey, Tsybrov, Fedor, Borshchevskiy, Valentin, Bukhalovich, Siarhei, V. Nazarenko, Vera, Kuznetsova, Elizaveta, Semenov, Oleg, Remeeva, Alina, and Gushchin, Ivan

Subjects
Flavoproteins, Flavin Mononucleotide, Riboflavin, protein engineering, Cell Biology, UV-Vis spectroscopy, flavin, Biochemistry, Luminescent Proteins, HEK293 Cells, microscopy, Flavin-Adenine Dinucleotide, Humans, fluorescence, Molecular Biology
Abstract

Flavin-binding fluorescent proteins are promising geneti-cally encoded tags for microscopy. However, spectral properties of their chromophores (riboflavin, flavin mononucleotide, and flavin adenine dinucleotide) are notoriously similar even be-tween different protein families, which limits applications of flavoproteins in multicolor imaging. Here, we present a palette of 22 finely tuned fluorescent tags based on the thermostable LOV domain from Chloroflexus aggregans. We performed site saturation mutagenesis of three amino acid positions in the flavin-binding pocket, including the photoactive cysteine, to obtain variants with fluorescence emission maxima uniformly covering the wavelength range from 486 to 512 nm. We demonstrate three-color imaging based on spectral separation and two-color fluorescence lifetime imaging of bacteria, as well as two-color imaging of mammalian cells (HEK293T), using the proteins from the palette. These results highlight the possibility of fine spectral tuning of flavoproteins and pave the way for further applications of flavin-binding fluorescent proteins in fluorescence microscopy. Generation and optical characterization of CagFbFP variants was supported by the Russian Science Foundation, grant number 21-64-00018. A. B. acknowledges the support of the Ministry of Science and Higher Education of the Russian Federation (agreement 075-03-2023-106, project FSMG-2020-0003) for fluorescence microscopy experiments.

Published
2022
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results