1. Comprehensive cancer-oriented biobanking resource of human samples for studies of post-zygotic genetic variation involved in cancer predisposition
- Author
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Kinga Drężek, Łukasz Bełch, Rafał Pęksa, Karol Sasim, Joanna Szpor, Marek Sierzega, Ewa Śrutek, Wojciech Zegarski, Edyta Rychlicka-Buniowska, Marcin Przewoźnik, Monika Horbacz, Łukasz Kaska, Arkadiusz Piotrowski, Dorian Wiśniewski, Krzysztof Okoń, Marek Zdrenka, Karol Tkaczyński, Michał Jankowski, Agnieszka Adamczyk, Dariusz Bała, Katarzyna Chojnowska, Krzysztof Kamecki, Wiktoria Stańkowska, Aleksandra Ambicka, Łukasz Szylberg, Diana Hodorowicz-Zaniewska, Janusz Jaszczyński, Magdalena Wójcik, Urszula Ławrynowicz, Mikolaj Przydacz, Jakub Szymanowski, Jarosław Kobiela, Hanna Davies, Izabela Ratnicka, Karol Wierzba, Janusz Ryś, Stanisław Hać, Jarosław Skokowski, Natalia Filipowicz, Jerzy Jankau, Agata Wojdak, Ulana Juhas, Andrzej Hellmann, Marta Drzewiecka, Wojciech Makarewicz, Piotr Remiszewski, Paweł Wroński, Karol Polom, Michał Bieńkowski, Katarzyna Duzowska, Jerzy Siekiera, Marcin Matuszewski, Jacek Kowalski, Jan P. Dumanski, Katarzyna Milian-Ciesielska, Maria Andreou, Olga Rostkowska, Tomasz Nowikiewicz, Agnieszka Harazin-Lechowska, and Piotr Chlosta
- Subjects
Oncology ,medicine.medical_specialty ,business.industry ,Colorectal cancer ,medicine.medical_treatment ,Cancer ,medicine.disease ,Cystectomy ,medicine.anatomical_structure ,Prostate ,Internal medicine ,Carcinoma ,medicine ,Breast-conserving surgery ,business ,Breast carcinoma ,Mastectomy - Abstract
The progress in translational cancer research relies on access to well-characterized samples from a representative number of patients and controls. The rationale behind our biobanking are explorations of post-zygotic pathogenic gene variants, especially in non-tumoral tissue, which might predispose to cancers. The targeted diagnoses are carcinomas of the breast (via mastectomy or breast conserving surgery), colon and rectum, prostate, and urinary bladder (via cystectomy or transurethral resection), exocrine pancreatic carcinoma as well as metastases of colorectal cancer to the liver. The choice was based on the high incidence of these cancers and/or frequent fatal outcome. We also collect age-matched normal controls. Our still ongoing collection originates from five clinical centers and after nearly 2-year cooperation reached 1711 patients and controls, yielding a total of 23226 independent samples, with an average of 74 donors and 1010 samples collected per month. The predominant diagnosis is breast carcinoma, with 933 donors, followed by colorectal carcinoma (383 donors), prostate carcinoma (221 donors), bladder carcinoma (81 donors), exocrine pancreatic carcinoma (15 donors) and metachronous colorectal cancer metastases to liver (14 donors). Forty percent of the total sample count originates from macroscopically healthy cancer-neighboring tissue, while contribution from tumors is 12%, which adds to the uniqueness of our collection for cancer predisposition studies. Moreover, we developed two program packages, enabling registration of patients, clinical data and samples at the participating hospitals as well as the central system of sample/data management at coordinating center. The approach used by us may serve as a model for dispersed biobanking from multiple satellite hospitals. Our biobanking resource ought to stimulate research into genetic mechanisms underlying the development of common cancers. It will allow all available “-omics” approaches on DNA-, RNA-, protein- and tissue levels to be applied. The collected samples can be made available to other research groups.
- Published
- 2021