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Your search keyword '"Paşca, Sergiu P."' showing total 6 results
Start Over Author "Paşca, Sergiu P." Publisher cold spring harbor laboratory
6 results on '"Paşca, Sergiu P."'

2. Kirigami electronics for long-term electrophysiological recording of human neural organoids and assembloids

Author

Yang, Xiao, primary, Forró, Csaba, additional, Li, Thomas L., additional, Miura, Yuki, additional, Zaluska, Tomasz J., additional, Tsai, Ching-Ting, additional, Kanton, Sabina, additional, McQueen, James P., additional, Chen, Xiaoyu, additional, Mollo, Valentina, additional, Santoro, Francesca, additional, Paşca, Sergiu P., additional, and Cui, Bianxiao, additional

Published
2023
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3. Generating human neural diversity with a multiplexed morphogen screen in organoids

Author

Amin, Neal D., Kelley, Kevin W., Hao, Jin, Miura, Yuki, Narazaki, Genta, Li, Tommy, McQueen, Patrick, Kulkarni, Shravanti, Pavlov, Sergey, and Paşca, Sergiu P.

Subjects
Article
Abstract

Morphogens choreograph the generation of remarkable cellular diversity in the developing nervous system. Differentiation of stem cells toward particular neural cell fates in vitro often relies upon combinatorial modulation of these signaling pathways. However, the lack of a systematic approach to understand morphogen-directed differentiation has precluded the generation of many neural cell populations, and knowledge of the general principles of regional specification remain in-complete. Here, we developed an arrayed screen of 14 morphogen modulators in human neural organoids cultured for over 70 days. Leveraging advances in multiplexed RNA sequencing technology and annotated single cell references of the human fetal brain we discovered that this screening approach generated considerable regional and cell type diversity across the neural axis. By deconvoluting morphogen-cell type relationships, we extracted design principles of brain region specification, including critical morphogen timing windows and combinatorics yielding an array of neurons with distinct neuro-transmitter identities. Tuning GABAergic neural subtype diversity unexpectedly led to the derivation of primate-specific interneurons. Taken together, this serves as a platform towards an in vitro morphogen atlas of human neural cell differentiation that will bring insights into human development, evolution, and disease.

Published
2023

6. Chromatin and gene-regulatory dynamics of the developing human cerebral cortex at single-cell resolution

Author

Trevino, Alexandro E., primary, Müller, Fabian, additional, Andersen, Jimena, additional, Sundaram, Laksshman, additional, Kathiria, Arwa, additional, Shcherbina, Anna, additional, Farh, Kyle, additional, Chang, Howard Y., additional, Paşca, Anca M., additional, Kundaje, Anshul, additional, Paşca, Sergiu P., additional, and Greenleaf, William J., additional

Published
2020
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