1. PHYTOSYNTHESIS OF ZINC OXIDE NANOPARTICLES WITH ACETONIC EXTRACT OF FLOWERS OF GERANIUM ROBERTIANUM L. (GERANIACEAE).
- Author
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Suručić, Relja, Šmitran, Aleksandra, Gajić, Dragana, Božić, Ljiljana, Antić, Marina, Topić-Vučenović, Valentina, Umičević, Nina, Antunović, Vesna, and Jelić, Dijana
- Subjects
ZINC oxide ,NANOPARTICLES ,SUSTAINABLE chemistry ,GERANIUM robertianum ,ANTI-infective agents - Abstract
Chemical and physical stability, high adsorption capacity and low-toxicological profile are very important features for application of nanoparticles (NPs) in the biomedical field. Physicochemical and biochemical characteristics of nanoparticles are very much dependent on the synthesis method. One of the promising, environmentally conscious method is the green synthesis of NPs using plant extracts. The aim of the this research was to present new plant extract for the synthesis of zinc oxide nanoparticles (ZnO NPs) and to test antimicrobial activity of in this way obtained ZnO NPs. In this study acetonic extract of flowers of Geranium robertianum L. (Geraniaceae) was used for phytosynthesis of zinc oxide nanoparticles (ZnO NPs). G. robertianum aerial parts are a good source of plant phenolics, particularly flavonoids which have an important role in metal nanoparticle synthesis. Total flavonoid content (4.2%) of acetonic extract used in ZnO NPs synthesis was determined by aluminum chloride spectrophotometric assay on 425 nm. Characterization of ZnO nanoparticles obtained by phytosynthesis procedure was done using UV/VIS spectrophotometry and IC spectroscopy. Obtained ZnO NPs were also tested as potential antimicrobial agents, considering that microorganisms rarely develop antimicrobial resistance against NPs. The Muller-Hinton agar well diffusion method was used for evaluation of ZnO NPs antimicrobial activity against Gram negative (Escherichia coli, Acinetobacter baumannii, and Pseudomonas aeruginosa) and Gram positive (three clinical isolates of Staphylococcus aureus) bacteria. The total flavonoid content of acetonic extract of flowers of G. robertianum used for ZnO NPs synthesis was 4.2%. The size of obtained ZnO NPs was approximately 40 nm. All three S. aureus isolates exhibited sensitivity (average value of inhibition zones around 15 mm), while Gram-negative bacteria were resistant to ZnO NPs activity. The results from our study confirm that acetonic extract of flowers of G. robertianum can be used for synthesis of ZnO NPs with significant antimicrobial activity towards Gram-positive bacteria. [ABSTRACT FROM AUTHOR]
- Published
- 2021