1. Genetic diversity in Tunisian horse breeds
- Author
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Faten Lasfer, B. Jemmali, Mohamed Habib Ezzaouia, Imen Gritli, Mohamed Mezir Haddad, Syrine Tounsi, Nawel Barhoumi, B. Rekik, Hatem Ouled Ahmed, Soufiene Ezzar, Amira Trabelsi, Belgacem Ben Aoun, and Abdelhak Ben Younes
- Subjects
0106 biological sciences ,0301 basic medicine ,Cultural Studies ,Veterinary medicine ,Genetic diversity ,lcsh:S ,Religious studies ,Horse ,Locus (genetics) ,Biology ,010603 evolutionary biology ,01 natural sciences ,Breed ,lcsh:Agriculture ,03 medical and health sciences ,030104 developmental biology ,lcsh:Zoology ,Genetic structure ,Microsatellite ,lcsh:Q ,lcsh:Animal culture ,lcsh:QL1-991 ,Allele ,lcsh:Science ,lcsh:SF1-1100 - Abstract
This study aimed at screening genetic diversity and differentiation in four horse breeds raised in Tunisia, the Barb, Arab-Barb, Arabian, and English Thoroughbred breeds. A total of 200 blood samples (50 for each breed) were collected from the jugular veins of animals, and genomic DNA was extracted. The analysis of the genetic structure was carried out using a panel of 16 microsatellite loci. Results showed that all studied microsatellite markers were highly polymorphic in all breeds. Overall, a total of 147 alleles were detected using the 16 microsatellite loci. The average number of alleles per locus was 7.52 (0.49), 7.35 (0.54), 6.3 (0.44), and 6 (0.38) for the Arab-Barb, Barb, Arabian, and English Thoroughbred breeds, respectively. The observed heterozygosities ranged from 0.63 (0.03) in the English Thoroughbred to 0.72 in the Arab-Barb breeds, whereas the expected heterozygosities were between 0.68 (0.02) in the English Thoroughbred and 0.73 in the Barb breeds. All FST values calculated by pairwise breed combinations were significantly different from zero (p
- Published
- 2017