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Discontinuity surfaces are well known to influence the mechanical behaviour of rocks under compression. Non-destructive techniques, such as ultrasonic pulse velocity and sclerometers, are increasingly used to estimate uniaxial compressive strength of rocks. In this study, several core samples derived from the doubling works of the railway network near Genga (Marche Region, Central Italy) were analysed in order to assess the influence of the structural geological context (proximity to folds, faults etc.) and tectonic deformation on rock strength. Tests were conducted in rock specimens through: i) conventional uniaxial compressive experiment, ii) non-destructive rebound-based methods such as Schmidt Hammer and Equotip and iii) ultrasound. In this way, it was possible to make a critical analysis of the use of these techniques in the estimation of the uniaxial compressive strength (considering also information about discontinuity type, orientation and nature of the filling). Finally, a petrographic analysis using optical microscope has been undertaken as a support to the observations derived from the analysis at the sample scale. The results indicate that there are two main factors influencing the strength at the scale of the specimen. The first and most decisive factor is the presence of natural pre-existing fractures. The second is the tectonic deformation ratio: the greater the deformation is, the little the strength. Furthermore, through the combined use of uniaxial compressive experiment, non-destructive rebound-based methods and ultrasounds it was possible to highlights the advantages and limitations of each technique and define/propose new guidelines for their use.