1. A cylinder-plate method for microbiological assay of clavulanic acid.
- Author
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Hamedi J, Shahverdi AR, Samadi N, Mohammadi A, Shiran M, and Akhondi S
- Subjects
- Agar, Anti-Bacterial Agents pharmacology, Calibration, Clavulanic Acid pharmacology, Escherichia coli growth & development, Penicillin G pharmacology, Pharmaceutical Preparations standards, Quality Control, Reproducibility of Results, Anti-Bacterial Agents analysis, Chromatography, High Pressure Liquid, Clavulanic Acid analysis, Escherichia coli drug effects, Microbial Sensitivity Tests, Technology, Pharmaceutical methods
- Abstract
Clavulanic acid is a natural occurring beta-lactam product of Streptomyces clavuligerus and is a potent inhibitor of bacterial beta-lactamases. The present work reports a microbiological assay based on the cylinder-plate method for determination of clavulanic acid. The assay is based on the inhibitory effect of clavulanic acid in combination with penicillin G upon Escherichia coli ATCC 35218, which is used as the test organism. The correlation between clavulanic acid concentration and the inhibitory effect on E. coli was linear (r > 0.99) and in the range of 8-20 microg/ml. These results indicate that the proposed method is appropriate for the determination of clavulanic acid in commercial samples and can be used in routine quality control.
- Published
- 2006