1. Biodegradation of Acid Scarlet 3R by a New Salt-tolerant Strain Alcaligenes faecalis LJ-3: Character, Enzyme and Kinetics Analysis.
- Author
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Song, X. Y., Liu, F. J., Zhou, H. B., and Yang, H. L.
- Subjects
ALCALIGENES faecalis ,BIODEGRADATION ,ENZYME analysis ,MICHAELIS-Menten mechanism ,AZO dyes - Abstract
Alcaligenes faecalis LJ-3 is highly efficient in degrading various azo dyes (100 mg L
-1 ). Almost 100 % degradation was observed within 16 h at the initial Acid Scarlet 3R concentrations of 1000 mg L-1 under the optimal conditions, which were: 1 g dextrin L-1 , 3 g yeast extract L-1 , NaCl ≤ 30 g L-1 , pH 8.0 and 35-45 ºC. Azoreductase, laccase and NADH-DCIP (nicotinamide adenine dinucleotide-dichlorophenol indophenols) reductase were induced during the degradation of Acid Scarlet 3R. Kinetics study of degradation experiments approximated the first-order reaction. The maximum rate (Vmax ) and substrate affinity constant (Ks ) were found to be 115.90 mg L-1 h-1 and 1193.23 mg L-1 , respectively, using Michaelis-Menten kinetics. This work provides new data characterizing Acid Scarlet 3R degradation by bacteria, as well as practical application potential in biological treatment of industrial effluents containing various azo dyes. [ABSTRACT FROM AUTHOR]- Published
- 2018
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