1. Altered expression of circular RNA in human dental pulp cells during odontogenic differentiation.
- Author
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Li C and Jiang H
- Subjects
- Adolescent, Adult, Bicuspid, Child, Dental Pulp cytology, Dental Pulp metabolism, Extracellular Matrix Proteins genetics, Extracellular Matrix Proteins metabolism, Female, Gene Expression Regulation, Gene Ontology, High-Throughput Nucleotide Sequencing, Humans, Male, Molar, Molecular Sequence Annotation, Odontoblasts cytology, Phosphoproteins genetics, Phosphoproteins metabolism, Primary Cell Culture, RNA, Circular classification, RNA, Circular metabolism, Sequence Analysis, RNA, Sialoglycoproteins genetics, Sialoglycoproteins metabolism, Signal Transduction, Tooth Extraction, Cell Differentiation genetics, Odontoblasts metabolism, Odontogenesis genetics, RNA, Circular genetics
- Abstract
The alterations in expression and function of circular RNA (circRNA) in human dental pulp cells (hDPCs) during odontogenic differentiation were investigated. To induce odontogenic differentiation, hDPCs (passage 3) were cultured for 14 days in odontogenic induction medium. circRNA high‑throughput sequencing was performed using Illumina HiSeqseq™ 2000. Subsequently, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were used to evaluate the bio‑functions of the identified circRNAs. To validate the results of circRNA sequencing, reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was performed for two selected differentially expressed circRNAs. The RNA sequencing results revealed that 1,314 and 1,780 circRNAs were upregulated and downregulated, respectively, during odontogenic induction. Their predicted target miRNAs and genes are involved in several biological functions and signaling pathways, including the mitogen‑associated protein kinase signaling pathway. The RT‑qPCR results of the two selected circRNAs (hsa_circ_0015260 and hsa_circ_0006984) were consistent with the expression trend obtained using high‑throughput sequencing. The results of the present study add to the current understanding of the regulatory mechanisms underlying hDPCs differentiation.
- Published
- 2019
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