1. PAQR3 gene expression and its methylation level in colorectal cancer tissues
- Author
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Lian-Jing Wang, Hao-Ran Zhang, Shuang Li, Jian-Wei Shi, Ya-Chao Chen, Wei Li, Teng-Yao Wei, Ri-Heng Li, Mei-Yu Tang, Yuan Chen, Ying Gao, Ai-Min Zhang, Hong-Ying Tang, Xiao-Rui Liu, and Tian-Yang Li
- Subjects
0301 basic medicine ,Cancer Research ,Pathology ,medicine.medical_specialty ,Colorectal cancer ,mRNA ,colorectal cancer ,Biology ,PAQR3 ,03 medical and health sciences ,0302 clinical medicine ,Western blot ,Gene expression ,medicine ,Oncogene ,medicine.diagnostic_test ,Methylation ,Articles ,Cell cycle ,medicine.disease ,Molecular medicine ,030104 developmental biology ,Oncology ,030220 oncology & carcinogenesis ,Cancer research ,western blot analysis ,methylation ,Infiltration (medical) - Abstract
The aim of the present study was to investigate the PAQR3 gene expression and its methylation level in colorectal cancer tissues, as well as the association with colorectal cancer clinical data. In total, 54 cases of colorectal cancer tissue samples and normal adjacent tissue samples were collected between June, 2013 and July, 2014. RT-PCR and western blot analysis were used to detect the mRNA and protein levels of PAQR3 in colorectal samples, respectively. MSP was used to detect the methylation level of PAQR3 gene in colorectal samples, which was compared with colorectal data. The results showed that a decreased expression level of PAQR3 mRNA in colorectal cancer tissues and the expression reduction rate was 57.4% (31/54). Similarly, the expression level of PAQR3 protein was reduced in cancer tissues, and the reduction rate was 46.3% (25/54), while the protein expression reduction rate in cancer adjacent tissue was 5.6% (3/54), and the difference was statistically significant (P
- Published
- 2016