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The ability of boron to diffuse from fused boron rods into surrounding wood was investigated on pentachlorophenol-treated Douglas-fir poles. Boron readily diffused into the wood surrounding the treatment holes and was present at protective levels in most poles within 1 year after application. The protected zone was generally confined to the treatment zone. Effective levels of boron were still present in this zone 15 years after treatment. Attempts to correlate the presence of decay fungi with residual boron levels indicated that these fungi were sometimes present in zones with boron at the lower threshold level of 0.5 kg m-3 boric acid equivalent (BAE), but most of the isolations could be explained by localized variations in distribution. Boron rods provided excellent long term protection against internal decay in Douglas-fir poles. [ABSTRACT FROM AUTHOR]

Clitocypin from the basidiomycete Clitocybe nebularis is the first fungal protein cysteine protease inhibitor to be characterised in detail, yet no information on its molecular genetics is available. Owing to its unique characteristics, it was assigned as the only member of a new family of cysteine protease inhibitors in the MEROPS inhibitor classification. Here we describe the full-length sequence of the clitocypin gene. A BLAST search confirmed its lack of significant sequence similarity to any other gene. The gene is composed of four exons and three short introns and belongs to a small family of closely related genes with more than 90% identity. Sequence variability is evenly distributed in introns and exons and deduced amino acid substitutions are distributed throughout the protein sequence. Basidiocarps collected at two distant locations were examined and the level of heterogeneity found in one basidiocarp is similar to that between the two. Sequencing of the ribosomal DNA spacers from the two basidiocarps confirmed that the heterogeneity observed in the clitocypin gene is not due to evolutionary divergence of the two specimens caused by geographic separation. Clitocypin is expressed in different parts of the basidiocarp and in cultured mycelia in a manner suggesting regulation by developmental and/or environmental factors. [ABSTRACT FROM AUTHOR]