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The purpose of this study was to investigate barriers faced by Pakistani university libraries to implement digital preservation programs. A questionnaire was developed and pilot tested before being sent to 70 libraries of the public sector universities and degree awarding institutions to measure the phenomena. Out of the targeted 70 libraries, 63 responded to the survey (90 % response rate). The results highlighted that a lack of institutional/national policies, financial and technological resources, and administrative support are key barriers that hinder the implementation of digital preservation practices in Pakistani university libraries. The findings of this novel study emphasize devising a national policy for digital preservation in Pakistan. Results point out key barriers to digital preservation and help designing knowledge-based systems in order to meet information and knowledge needs of library users around the clock. [ABSTRACT FROM AUTHOR]