1. A way to identify archaellins in Halobacterium salinarum archaella by FLAG-tagging
- Author
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Pavan S. Veluri, S. N. Beznosov, M. G. Pyatibratov, O. V. Fedorov, and Sagar Mitra
- Subjects
Identification ,QH301-705.5 ,Biology ,Methanococcus-Voltae ,flagellin ,General Biochemistry, Genetics and Molecular Biology ,Insert (molecular biology) ,Transformation ,Protein filament ,Halobacterium salinarum ,Biology (General) ,Iv Pili ,Gene ,haloarchaea ,Cleavage ,General Immunology and Microbiology ,Oligonucleotide ,General Neuroscience ,archaeal flagella ,Proteins ,food and beverages ,immuno-electron microscopy ,biology.organism_classification ,Molecular biology ,Cell biology ,Genes ,Multigene Family ,Haloarchaea ,biology.protein ,Filament ,General Agricultural and Biological Sciences ,Flagellin ,Bacterial Flagella ,Flag (geometry) - Abstract
In the current study, haloarchaea Halobacterium salinarum cells were transformed individually with each of the modified archaellin genes (flaA1, flaA2 and flaB2) containing an oligonucleotide insert encoding the FLAG peptide (DYKDDDDK). The insertion site was selected to expose the FLAG peptide on the archaella filament surface. Three types of transformed cells synthesizing archaella, containing A1, A2, or B2 archaellin modified with FLAG peptide were obtained. Electron microscopy of archaella has demonstrated that in each case the FLAG peptide is available for the specific antibody binding. It was shown for the first time that the B2 archaellin, like archaellins A1 and A2, is found along the whole filament length.
- Published
- 2013