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Copyright of Turkish Journal of Biochemistry / Turk Biyokimya Dergisi is the property of De Gruyter and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Turkish Journal of Biochemistry / Turk Biyokimya Dergisi is the property of De Gruyter and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Aims: To explore effect of Ca2+ and Cd2+ on the calmodulin (CaM), one complete cDNA sequence (AwCaM1) was cloned and characterized from the freshwater mussel Anodonta woodiana and its expressions were analyzed. Materials and methods: The AwCaM1 was cloned from the A. woodiana using the rapid amplification of cDNA ends methods and its expression was determined by real-time PCR. Results: In the hepatopancreas, AwCaM1 expression was up-regulated with a time and dose dependent pattern in the Ca2+ treated groups (0.01, 0.02, 0.04 and 0.08 mg/L) during experiment observed, and increased more than 56.15% (p<0.05) compared with that of control group. AwCaM1 mRNA level increased more 65.04% (p<0.05) in the Cd2+ treated groups (8 and 16 mg/L). In the gill, AwCaM1 expression increased more than 79.41% (p<0.05) compared with that of control group in all the Ca2+ treated groups, and more than 88.23% (p<0.05) in all the Cd2+ treated groups. Conclusion: These results indicated that up-regulations of AwCaM1 expression in bivalve A. woodiana are associated with Ca2+ absorb and environmental adaption derived from Ca2+ and Cd2+ treatment. [ABSTRACT FROM AUTHOR]