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The biometrical observations on the heart of fowl (vencobb-400 strain) revealed that the average weight was 8.91 ± 0.25 g, the mean percentage ratio between the length of rostral and caudal borders was 54:46 and the circumference at the coronary groove was 6.92 ± 0.08 cm. The right and left longitudinal grooves anastomosed with each other 1.05 ± 0.09 cm above the apex of the heart on the right side. The distance between the termination of caudal venacava and the apex of the heart was less (3.38 ± 0.13 cm) than the distance between the latter and origin of pulmonary artery (3.83 ± 0.13 cm). The cardiac skeleton was characterized by the presence of hyaline cartilage. The rostrocaudal diameter of the base of heart was greater (3.12 ± 0.08 cm) than the transverse diameter (2.15 ± 0.09 cm). The cross-sectional area of common aorta (46.00 ± 3.03 mm2) was greater than the area of pulmonary artery (32.4 ± 2.16 mm2). The total area of two anterior aortae (8.47 mm2) was lesser than the area of caudal aorta (10.8 mm2). The moderator bands could not be seen in both the ventricles.