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Shu-De Feng,1,* Ziming Mao,2,* Chunying Liu,2,* Yu-Song Nie,1 Bin Sun,2 Minggao Guo,3 Changqing Su2 1Department of General Surgery, Jiangsu Armed Police General Hospital, Yangzhou, Jiangsu, China; 2Department of Molecular Oncology, Eastern Hepatobiliary Surgical Hospital, National Center of Liver Cancer, Second Military Medical University, Shanghai, China; 3Department of General Surgery, Shanghai Sixth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China *These authors contributed equally tothis work Background: Pancreatic adenocarcinoma (PAC) is one of the most fatal cancers due to its high degree of malignancy, increasing incidence, high mortality, and unsatisfactory treatment efficacy. Evidence has suggested that numerous microRNAs (miRNAs), including miR-126 and miR-34a, have potent tumor-suppressing effects on PAC, implicating a possible application of miRNA in tumor therapy. However, the therapeutic effect of a single miRNA on pancreatic cancer is limited.Methods: We simultaneously delivered miR-126 and miR-34a into PAC cells by a carcinoem­bryonic antigen promoter-driven oncolytic adenovirus (AdCEAp-miR126/34a), and examined the antitumor efficacy of the therapeutic system in in vitro and in vivo experiments.Results: In vitro cytological experiments found that the expression levels of miR-126 and miR-34a were specifically increased in the AdCEAp-miR126/34a-infected PAC cells, and the antitumor efficacy was enhanced in aspects of cancer cell viability, migration, invasion, and apoptosis, by synergistically combining the antitumor effects of overexpressed miR-126 and miR-34a and the oncolytic effect of viral replication specifically in PAC cells. The expression levels of miR-126 target genes (vascular endothelial growth factor-A and SOX2) and miR-34a target genes (cyclin D1, E2F1, and Bcl-2) were markedly decreased in the PAC cells after being infected with AdCEAp-miR126/34a. Notable suppression of the therapeutic system on tumor growth was also proven in established PAC xenograft tumor models in nude mice, which demonstrated that the combination of miR-126 and miR-34a exerts more effective antitumor outcomes than a single miRNA.Conclusion: The therapeutic system co-expressing miR-126 and miR-34a mediated by oncolytic adenovirus is a promising system for PAC target therapy. Keywords: pancreatic cancer, miR-126, miR-34a, oncolytic adenovirus, target therapy

Guang-Xian Zhong,1 Shao-Dan Feng,2 Rong-Kai Shen,1 Zhao-Yang Wu,1 Fei Chen,1 Xia Zhu1 1Department of Orthopaedics, 2Department of Emergency, The First Affiliated Hospital of Fujian Medical University, Fuzhou, People’s Republic of China Purpose: The aim of this study was to investigate the clinical significance of circulating tumor cells (CTCs) in the peripheral blood of an osteosarcoma and the Ezrin gene expressed in CTCs.Patients and methods: CTC enrichment was done with CanPatrol™ CTC enrichment technique in 41 patients with osteosarcoma. The characterization of CTCs was performed using a multiple messenger RNA in situ analysis (MRIA). The expression of the Ezrin gene in CTCs was detected by RNA probe technology. The correlations of CTC counts, cell type and the expression level of the Ezrin gene with clinical stage and metastasis of osteosarcoma were analyzed using SPSS 16.0 software.Results: The CTC counts correlated significantly with Enneking stage (P

Feng S-D, Mao Z, Liu Chunying, Nie Y-S, Sun B, Guo M,Su C. Simultaneous overexpression of miR-126 and miR-34a induces a superior antitumor efficacy in pancreaticadenocarcinoma. Onco Targets Ther. 2017;10:5591–5604.The Editor-in-Chief and Publisher of OncoTargets andTherapy wish to retract the published article. Concerns were raised to the Editor-in-Chief regarding thepossibility of some image manipulation in the publishedarticle. The alleged manipulation relates to Figure 2AAdCEAp-EGFP Panc-1 and AdCEAp-EGFP BxPC3. Itappears the same image had been used in both panels butwith the fluorescence intensity altered.The authors were asked for an explanation but were unableto provide a satisfactory explanation for the duplicatedimages and could not clearly account for the altered fluorescenceintensity. The Editor-in-Chief raised doubts about the validity of theresults and determined that simply replacing the images inquestion not been adequately justified by the authors. The authors wish to apologise for the error. Our decision-making was informed by our policy on publishingethics and integrity and the COPE guidelines onretraction. The retracted article will remain online to maintain thescholarly record, but it will be digitally watermarked oneach page as “Retracted”. This retraction relates to this paper