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Lin-Yu Jin,1,2,&ast; Xin-Jin Su,3,&ast; Shuai Xu,1 Hai-Ying Liu,1 Xin-Feng Li2 1Department of Spinal Surgery, Peking University People’s Hospital, Peking University, Beijing, People’s Republic of China; 2Department of Orthopedics, Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopedics, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, People’s Republic of China; 3Department of Spinal Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Xin-Feng Li, Department of Orthopedics, Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopedics, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200025, People’s Republic of China, Email lxfrenji@126.com Hai-Ying Liu, Department of Spinal Surgery, Peking University People’s Hospital, Peking University, No. 11, South Xizhimen Street, Beijing, 100044, People’s Republic of China, Email drlhypkuph@163.comObjective: Hounsfield Unit (HU) has been used to investigate the asymmetrical vertebral bone mass in patients associated with adult degenerative scoliosis (ADS). Therefore, there is an inevitable need to evaluate the performance of HU values in ADS subjects.Methods: A total of 162 patients (81 ADS patients and 81 non-ADS patients) aged ≥ 50 years undergoing the CT examination were reviewed. The HU values of the lumbar vertebral body (including total, convex side, and concave side) at bilateral pedicle plane were obtained and compared. The paired t-test, chi-squared test, independent samples t-test, and interclass correlation coefficient (ICC) were used for statistical analyses.Results: The HU values were significantly different between the convex and concave sides of the lumbar vertebral body (P < 0.01). The total prevalence of osteoporosis (OP) in ADS patients was higher than that of non-ADS patients. The prevalence of OP in female or male of ADS patients was higher than that of non-ADS patients, respectively. Intra- and inter-rater reliability were very strong (both > 0.8) for measuring asymmetrical vertebral bone mass in ADS patients.Conclusion: HU value was a high reproducibility method for evaluating the vertebral bone mass in ADS patients. The HU values at the concave sides were significantly higher than that of convex sides at the lumbar vertebral body on the pedicle plane. The prevalence of OP in ADS patients was higher than that of non-ADS patients, especially for females associated with ADS. Moreover, the static asymmetric load did not enhance the bone mass at the concave side compared with the left/right side of non-ADS patients.Keywords: adult degenerative scoliosis, bone mass, Hounsfield units, reliability

Jie Hu,1,2 Xin-juan Su,2 Hui-Ling Si,2 Rui-Xiang Song,3 Fang Zhang,3 Xiang-Jun Qiu,3 Xing-Peng Chen2 1Department of Anesthesiology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, 710049, People’s Republic of China; 2Department of Anesthesiology, Luoyang Central Hospital, Luoyang, Henan Province, 471003, People’s Republic of China; 3School of Basic Medical Sciences, Henan University of Science and Technology, Luoyang, 471023, People’s Republic of ChinaCorrespondence: Xiang-Jun QiuSchool of Basic Medicine, Henan University of Science and Technology, Luoyang, 471023, People’s Republic of ChinaEmail lyxiangjun@126.comXing-Peng ChenLuoyang Central Hospital, Luoyang, Henan Province, 471003, People’s Republic of ChinaEmail lycxp2020@126.comBackground: An efficient, fast and sensitive ultra high-performance liquid chromatography-mass spectrometry (UPLC-MS/MS) method for simultaneous determination of celecoxib (CEL), dezocine (DEZ) and dexmedetomidine (DEX) in beagle plasma were established.Methods: The beagle dogs plasmawas precipitated by acetonitrile. The column was Acquity UPLC BEH C18 column and the mobile phase was acetonitrile-formic acid with gradient mode, and the flow rate was set at 0.4 mL/min. Under the positive ion mode, CEL, DEZ, DEX and Midazolam (internal standard, IS) were monitored by multiple reaction monitoring (MRM) as the following mass transition pairs: m/z 381.10→ 282.10 for CEL, m/z 246.20→ 147.00 for DEZ, m/z 201.10→ 94.90 for DEX, and m/z 326.10→ 291.10 for IS.Results: This UPLC-MS/MS method had good linearity for CEL, DEZ and DEX. The RSDs of inter-day and intra-day precision were the values of 0.31– 7.66% and 0.11– 9.63%, respectively; the RE values were from − 6.05% to 10.98%. The extraction recovery was more than 79%, and the matrix effect was around 100%. The RSDs of stability were less than 8.96%. All of them met the acceptance standard of biological analysis method recommended by FDA.Conclusion: This UPLC-MS/MS method is an effective tool for the simultaneous determination of CEL, DEX and DEX, and has been successfully applied to the study of pharmacokinetics in beagle dogs.Keywords: celecoxib, dezocine, dexmedetomidine, UPLC-MS/MS, pharmacokinetics, beagles