1. Shorter Cilia Length and Aberrant Ciliated Marker DNAI1 in Allergic Rhinitis.
- Author
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Zhou S, Liu Y, Yang Y, Huang H, and Qiu Q
- Abstract
Purpose: This study aimed to investigate whether the impaired ciliary length and aberrant ciliary ultrastructure marker, dynein axonemal intermediate chain 1 (DNAI1), are important pathological characteristics in nasal mucosa from patients with allergic rhinitis (AR)., Patients and Methods: Biopsies were taken from the inferior turbinate (IT) of controls (n = 20) and patients with AR (n = 20). The ciliary length and the DNAI1 location patterns were assessed by using immunofluorescent staining. Three patterns of DNAI1 localization were defined using a semi-quantitative scoring system: normal (N), partial (P) and absence (A). Every individual section was assigned a score between 0 and 2 in each high-power field (5 fields per sample). The score of 0 = pattern N >70%; 1 = patterns N + P >70%; and 2 = pattern A ≥30%. The receiver operating characteristic (ROC) curve was used to evaluate the predicted value of DNAI1 score for AR., Results: The ciliary length was reduced by 33.3% in patients with AR compared with controls ( P < 0.0001). The higher DNAI1 score was found in the AR group, with a median (first and third quartile) of 0.9 (0.4 and 1.08), which was 0.1 (0 and 0.76) in the control group ( P = 0.0071). The ROC of DNAI1 was calculated based on the area under the curve of 0.74 ( P = 0.0094). The cutoff value of ROC was 0.5833, with a sensitivity and specificity of 70%., Conclusion: These results suggested that the shorter ciliary length and aberrant localization of DNAI1 are potentially important pathological characteristics of the allergic nasal mucosa. The aberrant localization of DNAI1 may provide a novel candidate target for clinical management of AR., Competing Interests: The authors report no conflicts of interest in this work., (© 2023 Zhou et al.)
- Published
- 2023
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