1. Effect of Sterilization Methods on the Morphological, Molecular, and Biocompatibility Characteristics of Nanofibrous PEEK Layers
- Author
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Ivanovich Vatin Nikolai, Satyanarayana G.V.V., Anil Kalluri, Singh Negi Anup, Sharma Nittin, Brar Manvinder, and Sharma Diksha
- Subjects
peek ,nanofibrous layers ,sterilization ,autoclave ,ethylene oxide ,uv radiation ,morphology ,biocompatibility ,electrospinning ,Environmental sciences ,GE1-350 - Abstract
In this work, the polyether ether ketone (PEEK) nanofibrous layers and their sterilized methods influence on molecular properties, structural properties, and biocompatibility have been discussed.Some instrument employed were gamma radiation, ultraviolet light, Ethylene oxide (EtOx) and an autoclave to sterilised the layers developed through melt-electrospinning process.umber of instruments used such as gamma radiation, ultraviolet light, ethylene oxide (EtOx), and an autoclave was used to sterilise the layers prepared via melt-electrospinning process. The SEM result unveiled that the sterilization processes changed the fibre form slightly as well as the diameter which was modified slightly as well.However, the sterilization processes did not alter the molecular weight of PEEK fibers, as far as GPC study was concerned.d biocompatibility has been explored. A number of instruments used such as gamma radiation, ultraviolet light, ethylene oxide (EtOx), and an autoclave was used to sterilise the layers prepared via melt-electrospinning process. The scanning electron microscopy (SEM) showed that the sterilization processes slightly altered the fiber’s form along with small variations in diameter. While, the sterilization processes did not achanged the molecular weight of PEEK fibers, according to gel permeation chromatography (GPC) research. In addition, the murine fibroblasts (3T3) were assessed for in vitro biocompatibility using MTT test. While cell adhesion and proliferation rate were the same across all the layers in the sterile control sample, the UV-sterilized material significantly reduced the level of cell viability. Consequently, the employed methods, namely the autoclave, EtOx, and gamma radiation were determined to be efficient to sterilise PEEK nanofibrous layers. However, solar radiation with the method may also reduce their biocompatibility.
- Published
- 2024
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