1. SNX9-induced membrane tubulation regulates CD28 cluster stability and signalling.
- Author
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Ecker M, Schregle R, Kapoor-Kaushik N, Rossatti P, Betzler VM, Kempe D, Biro M, Ariotti N, Redpath GM, and Rossy J
- Subjects
- Animals, Humans, Immunological Synapses genetics, Immunological Synapses metabolism, Interleukin-2 genetics, Interleukin-2 metabolism, Jurkat Cells, Lymphocyte Activation genetics, Mice, Mice, Transgenic, Phosphorylation, Receptors, Antigen, T-Cell metabolism, T-Lymphocytes cytology, T-Lymphocytes metabolism, CD28 Antigens genetics, CD28 Antigens metabolism, Cell Membrane chemistry, Cell Membrane metabolism, Signal Transduction genetics, Sorting Nexins genetics, Sorting Nexins metabolism
- Abstract
T cell activation requires engagement of a cognate antigen by the T cell receptor (TCR) and the co-stimulatory signal of CD28. Both TCR and CD28 aggregate into clusters at the plasma membrane of activated T cells. While the role of TCR clustering in T cell activation has been extensively investigated, little is known about how CD28 clustering contributes to CD28 signalling. Here, we report that upon CD28 triggering, the BAR-domain protein sorting nexin 9 (SNX9) is recruited to CD28 clusters at the immunological synapse. Using three-dimensional correlative light and electron microscopy, we show that SNX9 generates membrane tubulation out of CD28 clusters. Our data further reveal that CD28 clusters are in fact dynamic structures and that SNX9 regulates their stability as well as CD28 phosphorylation and the resulting production of the cytokine IL-2. In summary, our work suggests a model in which SNX9-mediated tubulation generates a membrane environment that promotes CD28 triggering and downstream signalling events., Competing Interests: ME, RS, NK, PR, VB, DK, MB, NA, GR, JR No competing interests declared, (© 2022, Ecker et al.)
- Published
- 2022
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