1. A subcellular map of the human kinome
- Author
-
Qinghua Liao, Mei Tang, Haitao Zhang, Liangyi Chen, Shuaifeng Li, Guoxuan Zhong, Jia Feng, Jianhui Zhao, Chenliang Wang, Huasong Lu, Yuan Si, Liuju Li, Xiaolei Cao, Manuel Kaulich, Li Li, Feifeng Zhu, Fangwei Wang, Tingbo Liang, Bin Zhao, Zongping Xia, Xin-Hua Feng, and Haidong Li more...
- Subjects
0301 basic medicine ,QH301-705.5 ,Science ,Chemical biology ,Mitochondrion ,Biology ,General Biochemistry, Genetics and Molecular Biology ,Epitopes ,03 medical and health sciences ,0302 clinical medicine ,Biochemistry and Chemical Biology ,subcellular localization ,Humans ,Protein phosphorylation ,Kinome ,Phosphorylation ,Biology (General) ,Cellular compartment ,Organelles ,General Immunology and Microbiology ,MOK ,Kinase ,General Neuroscience ,kinome ,Cell Biology ,General Medicine ,mitochondrial ,Subcellular localization ,Mitochondria ,Tools and Resources ,Cell biology ,030104 developmental biology ,Microscopy, Fluorescence ,Medicine ,phase separation ,Protein Kinases ,030217 neurology & neurosurgery ,Function (biology) ,HeLa Cells ,Subcellular Fractions ,Human - Abstract
The human kinome comprises 538 kinases playing essential functions by catalyzing protein phosphorylation. Annotation of subcellular distribution of the kinome greatly facilitates investigation of normal and disease mechanisms. Here, we present Kinome Atlas (KA), an image-based map of the kinome annotated to 10 cellular compartments. 456 epitope-tagged kinases, representing 85% of the human kinome, were expressed in HeLa cells and imaged by immunofluorescent microscopy under a similar condition. KA revealed kinase family-enriched subcellular localizations and discovered a collection of new kinase localizations at mitochondria, plasma membrane, extracellular space, and other structures. Furthermore, KA demonstrated the role of liquid-liquid phase separation in formation of kinase condensates. Identification of MOK as a mitochondrial kinase revealed its function in cristae dynamics, respiration, and oxidative stress response. Although limited by possible mislocalization due to overexpression or epitope tagging, this subcellular map of the kinome can be used to refine regulatory mechanisms involving protein phosphorylation. more...
- Published
- 2021
- Full Text
- View/download PDF