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Your search keyword '"Sarah Hanzén"' showing total 2 results
Start Over Author "Sarah Hanzén" Publisher elife sciences publications, ltd
2 results on '"Sarah Hanzén"'

1. Author response: Peroxiredoxin promotes longevity and H2O2-resistance in yeast through redox-modulation of protein kinase A

Author

Sarah Hanzén, Thomas Nyström, Marouane Libiad, Markus Hartl, Chunxia Gao, Cecilia Picazo, Friederike Roger, Gilles Lagniel, Mikael Molin, Niek Welkenhuysen, Jean Labarre, Wolfgang Reiter, Michel B. Toledano, Morten Grøtli, and Chikako Asami

Subjects
Redox modulation, Chemistry, media_common.quotation_subject, Longevity, Protein kinase A, Peroxiredoxin, Yeast, media_common, Cell biology
Published
2020
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2. Peroxiredoxin promotes longevity and H2O2-resistance in yeast through redox-modulation of protein kinase A

Author

Cecilia Picazo, Friederike Roger, Niek Welkenhuysen, Chunxia Gao, Marouane Libiad, Markus Hartl, Mikael Molin, Sarah Hanzén, Thomas Nyström, Gilles Lagniel, Chikako Asami, Jean Labarre, Morten Grøtli, Wolfgang Reiter, Michel B. Toledano, Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Stress Oxydatif et Cancer (SOC), Département Biologie Cellulaire (BioCell), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)

Subjects
0301 basic medicine, inorganic chemicals, QH301-705.5, Science, Protein subunit, [SDV]Life Sciences [q-bio], S. cerevisiae, chemical biology, General Biochemistry, Genetics and Molecular Biology, cysteine sulfenylation, 03 medical and health sciences, 0302 clinical medicine, cell biology, biochemistry, Biology (General), Protein kinase A, biology, General Immunology and Microbiology, Chemistry, Kinase, General Neuroscience, aging, H2O2 signalling, peroxiredoxin, General Medicine, Cell biology, Cytosol, 030104 developmental biology, Chaperone (protein), biology.protein, Medicine, Phosphorylation, protein kinase A, Signal transduction, Peroxiredoxin, glutathionylation, 030217 neurology & neurosurgery
Abstract

Peroxiredoxins are H2O2scavenging enzymes that also carry out H2O2signaling and chaperone functions. In yeast, the major cytosolic peroxiredoxin, Tsa1 is required for both promoting resistance to H2O2and extending lifespan upon caloric restriction. We show here that Tsa1 effects both these functions not by scavenging H2O2, but by repressing the nutrient signaling Ras-cAMP-PKA pathway at the level of the protein kinase A (PKA) enzyme. Tsa1 stimulates sulfenylation of cysteines in the PKA catalytic subunit by H2O2and a significant proportion of the catalytic subunits are glutathionylated on two cysteine residues. Redox modification of the conserved Cys243 inhibits the phosphorylation of a conserved Thr241 in the kinase activation loop and enzyme activity, and preventing Thr241 phosphorylation can overcome the H2O2sensitivity of Tsa1-deficient cells. Results support a model of aging where nutrient signaling pathways constitute hubs integrating information from multiple aging-related conduits, including a peroxiredoxin-dependent response to H2O2.

Published
2020
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