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Your search keyword '"Spradling, Allan C."' showing total 21 results
Start Over Author "Spradling, Allan C." Publisher elife sciences publications, ltd
21 results on '"Spradling, Allan C."'

8. An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms

Author

Kanca, Oguz, primary, Zirin, Jonathan, additional, Garcia-Marques, Jorge, additional, Knight, Shannon Marie, additional, Yang-Zhou, Donghui, additional, Amador, Gabriel, additional, Chung, Hyunglok, additional, Zuo, Zhongyuan, additional, Ma, Liwen, additional, He, Yuchun, additional, Lin, Wen-Wen, additional, Fang, Ying, additional, Ge, Ming, additional, Yamamoto, Shinya, additional, Schulze, Karen L, additional, Hu, Yanhui, additional, Spradling, Allan C, additional, Mohr, Stephanie E, additional, Perrimon, Norbert, additional, and Bellen, Hugo J, additional

Published
2019
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9. Author response: An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms

Author

Kanca, Oguz, primary, Zirin, Jonathan, additional, Garcia-Marques, Jorge, additional, Knight, Shannon Marie, additional, Yang-Zhou, Donghui, additional, Amador, Gabriel, additional, Chung, Hyunglok, additional, Zuo, Zhongyuan, additional, Ma, Liwen, additional, He, Yuchun, additional, Lin, Wen-Wen, additional, Fang, Ying, additional, Ge, Ming, additional, Yamamoto, Shinya, additional, Schulze, Karen L, additional, Hu, Yanhui, additional, Spradling, Allan C, additional, Mohr, Stephanie E, additional, Perrimon, Norbert, additional, and Bellen, Hugo J, additional

Published
2019
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11. A gene-specific T2A-GAL4 library for Drosophila

Author

Lee, Pei-Tseng, primary, Zirin, Jonathan, additional, Kanca, Oguz, additional, Lin, Wen-Wen, additional, Schulze, Karen L, additional, Li-Kroeger, David, additional, Tao, Rong, additional, Devereaux, Colby, additional, Hu, Yanhui, additional, Chung, Verena, additional, Fang, Ying, additional, He, Yuchun, additional, Pan, Hongling, additional, Ge, Ming, additional, Zuo, Zhongyuan, additional, Housden, Benjamin E, additional, Mohr, Stephanie E, additional, Yamamoto, Shinya, additional, Levis, Robert W, additional, Spradling, Allan C, additional, Perrimon, Norbert, additional, and Bellen, Hugo J, additional

Published
2018
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12. Author response: A gene-specific T2A-GAL4 library for Drosophila

Author

Lee, Pei-Tseng, primary, Zirin, Jonathan, additional, Kanca, Oguz, additional, Lin, Wen-Wen, additional, Schulze, Karen L, additional, Li-Kroeger, David, additional, Tao, Rong, additional, Devereaux, Colby, additional, Hu, Yanhui, additional, Chung, Verena, additional, Fang, Ying, additional, He, Yuchun, additional, Pan, Hongling, additional, Ge, Ming, additional, Zuo, Zhongyuan, additional, Housden, Benjamin E, additional, Mohr, Stephanie E, additional, Yamamoto, Shinya, additional, Levis, Robert W, additional, Spradling, Allan C, additional, Perrimon, Norbert, additional, and Bellen, Hugo J, additional

Published
2018
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15. A library of MiMICs allows tagging of genes and reversible, spatial and temporal knockdown of proteins in Drosophila

Author

Nagarkar-Jaiswal, Sonal, primary, Lee, Pei-Tseng, additional, Campbell, Megan E, additional, Chen, Kuchuan, additional, Anguiano-Zarate, Stephanie, additional, Cantu Gutierrez, Manuel, additional, Busby, Theodore, additional, Lin, Wen-Wen, additional, He, Yuchun, additional, Schulze, Karen L, additional, Booth, Benjamin W, additional, Evans-Holm, Martha, additional, Venken, Koen JT, additional, Levis, Robert W, additional, Spradling, Allan C, additional, Hoskins, Roger A, additional, and Bellen, Hugo J, additional

Published
2015
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16. Author response: A library of MiMICs allows tagging of genes and reversible, spatial and temporal knockdown of proteins in Drosophila

Author

Nagarkar-Jaiswal, Sonal, primary, Lee, Pei-Tseng, additional, Campbell, Megan E, additional, Chen, Kuchuan, additional, Anguiano-Zarate, Stephanie, additional, Cantu Gutierrez, Manuel, additional, Busby, Theodore, additional, Lin, Wen-Wen, additional, He, Yuchun, additional, Schulze, Karen L, additional, Booth, Benjamin W, additional, Evans-Holm, Martha, additional, Venken, Koen JT, additional, Levis, Robert W, additional, Spradling, Allan C, additional, Hoskins, Roger A, additional, and Bellen, Hugo J, additional

Published
2015
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21. A gene-specific T2A-GAL4 library for Drosophila

Author

Lee, Pei-Tseng, Zirin, Jonathan, Kanca, Oguz, Lin, Wen-Wen, Schulze, Karen L, Li-Kroeger, David, Tao, Rong, Devereaux, Colby, Hu, Yanhui, Chung, Verena, Fang, Ying, He, Yuchun, Pan, Hongling, Ge, Ming, Zuo, Zhongyuan, Housden, Benjamin E, Mohr, Stephanie E, Yamamoto, Shinya, Levis, Robert W, Spradling, Allan C, Perrimon, Norbert, and Bellen, Hugo J

Subjects
MiMIC, CRIMIC, GAL4/UAS, complementation, cassette excision, loss of function
Abstract

We generated a library of ~1000 Drosophila stocks in which we inserted a construct in the intron of genes allowing expression of GAL4 under control of endogenous promoters while arresting transcription with a polyadenylation signal 3’ of the GAL4. This allows numerous applications. First, ~90% of insertions in essential genes cause a severe loss-of-function phenotype, an effective way to mutagenize genes. Interestingly, 12/14 chromosomes engineered through CRISPR do not carry second-site lethal mutations. Second, 26/36 (70%) of lethal insertions tested are rescued with a single UAS-cDNA construct. Third, loss-of-function phenotypes associated with many GAL4 insertions can be reverted by excision with UAS-flippase. Fourth, GAL4 driven UAS-GFP/RFP reports tissue and cell-type specificity of gene expression with high sensitivity. We report the expression of hundreds of genes not previously reported. Finally, inserted cassettes can be replaced with GFP or any DNA. These stocks comprise a powerful resource for assessing gene function.

Published
2018
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