1. ODELAM, rapid sequence-independent detection of drug resistance in isolates of Mycobacterium tuberculosis
- Author
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Tige R. Rustad, John D. Aitchison, Thurston Herricks, David R. Sherman, Magdalena Donczew, Timothy R Sterling, Robert Morrison, and Fred D. Mast
- Subjects
0301 basic medicine ,Drug ,QH301-705.5 ,Science ,media_common.quotation_subject ,030106 microbiology ,Drug resistance ,General Biochemistry, Genetics and Molecular Biology ,Microbiology ,Mycobacterium tuberculosis ,03 medical and health sciences ,Minimum inhibitory concentration ,Antibiotic resistance ,medicine ,Biology (General) ,media_common ,Colony-forming unit ,drug resistance ,General Immunology and Microbiology ,biology ,General Neuroscience ,General Medicine ,biology.organism_classification ,030104 developmental biology ,growth phenotype ,Medicine ,Ofloxacin ,heterogeneity ,medicine.drug ,Mycobacterium - Abstract
Antimicrobial-resistant Mycobacterium tuberculosis (Mtb) causes over 200,000 deaths each year. Current assays of antimicrobial resistance need knowledge of mutations that confer drug resistance, or long periods of culture time to test growth under drug pressure. We present ODELAM (One-cell Doubling Evaluation of Living Arrays of Mycobacterium), a time-lapse microscopy-based method that observes individual cells growing into microcolonies. ODELAM enables rapid quantitative measures of growth kinetics in as little as 30 hrs under a wide variety of environmental conditions. We demonstrate ODELAM’s utility by identifying ofloxacin resistance in cultured clinical isolates of Mtb and benchmark its performance with standard minimum inhibitory concentration (MIC) assays. ODELAM identified ofloxacin heteroresistance and the presence of drug resistant colony forming units (CFUs) at 1 per 1000 CFUs in as little as 48 hrs. ODELAM is a powerful new tool that can rapidly evaluate Mtb drug resistance in a laboratory setting. more...
- Published
- 2020
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