1. Adrenergic activation modulates the signal from the Reissner fiber to cerebrospinal fluid-contacting neurons during development
- Author
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Yasmine Cantaut-Belarif, Claire Wyart, Adeline Orts Del'Immagine, Guillaume Pézeron, Margot Penru, Pierre-Luc Bardet, Institut du Cerveau et de la Moëlle Epinière = Brain and Spine Institute (ICM), Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Physiologie moléculaire et adaptation (PhyMA), Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS), Institut du Cerveau = Paris Brain Institute (ICM), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), and Gestionnaire, Hal Sorbonne Université
- Subjects
Embryo, Nonmammalian ,[SDV]Life Sciences [q-bio] ,Gene Expression ,Adrenergic ,0302 clinical medicine ,Cerebrospinal fluid ,Morphogenesis ,Biology (General) ,Zebrafish ,Neurons ,0303 health sciences ,biology ,Chemistry ,General Neuroscience ,General Medicine ,3. Good health ,Cell biology ,[SDV] Life Sciences [q-bio] ,Medicine ,Signal Transduction ,Research Article ,Fish Proteins ,QH301-705.5 ,Science ,Neuropeptide ,Reissner fiber ,cerebrospinal fluid ,General Biochemistry, Genetics and Molecular Biology ,norepinephrine ,03 medical and health sciences ,CSF-contacting neurons ,Extracellular ,Animals ,epinephrine ,030304 developmental biology ,General Immunology and Microbiology ,Neuropeptides ,biology.organism_classification ,Embryonic stem cell ,Spinal Nerves ,SCO-spondin ,sense organs ,Developmental biology ,030217 neurology & neurosurgery ,Developmental Biology ,Neuroscience - Abstract
International audience; The cerebrospinal fluid (CSF) contains an extracellular thread conserved in vertebrates, the Reissner fiber, which controls body axis morphogenesis in the zebrafish embryo. Yet, the signaling cascade originating from this fiber to ensure body axis straightening is not understood. Here, we explore the functional link between the Reissner fiber and undifferentiated spinal neurons contacting the CSF (CSF-cNs). First, we show that the Reissner fiber is required in vivo for the expression of urp2, a neuropeptide expressed in CSF-cNs. We show that the Reissner fiber is also required for embryonic calcium transients in these spinal neurons. Finally, we study how local adrenergic activation can substitute for the Reissner fiber-signaling pathway to CSF-cNs and rescue body axis morphogenesis. Our results show that the Reissner fiber acts on CSF-cNs and thereby contributes to establish body axis morphogenesis, and suggest it does so by controlling the availability of a chemical signal in the CSF.
- Published
- 2020