1. SIN, a novel Drosophila protein that associates with the RNA binding protein sex-lethal.
- Author
-
Dong Z and Bell LR
- Subjects
- Amino Acid Sequence, Animals, Base Sequence, Binding Sites, Chromosome Mapping, DNA, Complementary chemistry, DNA, Complementary genetics, Drosophila metabolism, Embryo, Nonmammalian metabolism, Female, Gene Expression Regulation, Developmental, In Situ Hybridization, Insect Proteins metabolism, Molecular Sequence Data, Protein Binding, RNA, Messenger genetics, RNA, Messenger metabolism, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Tissue Distribution, Two-Hybrid System Techniques, Drosophila genetics, Drosophila Proteins, Insect Proteins genetics, RNA-Binding Proteins metabolism
- Abstract
Sex-lethal (SXL) is an RNA binding protein that acts as a regulator of both alternative pre-mRNA splicing and translation. Because SXL must sometimes function at some distance from its binding sites, it is believed that it must interact with other proteins. We used a yeast two-hybrid screen to isolate a novel Drosophila protein, SIN (SXL interactor), that interacts specifically with SXL. A direct physical association was demonstrated in vitro, and a single SXL RNA binding domain was sufficient for the interaction. SIN shows a high degree of similarity to a mammalian protein of unknown function. The cytogenetic location of Sin is 78A2-4. The transcript, which is abundant in early embryos, appears to be of maternal origin.
- Published
- 1999
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