1. Characterization of spaA, a Streptomyces coelicolor gene homologous to a gene involved in sensing starvation in Escherichia coli.
- Author
-
Schneider D, Bruton CJ, and Chater KF
- Subjects
- Amino Acid Sequence, Anthraquinones, Anti-Bacterial Agents biosynthesis, Bacterial Proteins metabolism, Chromosome Mapping, Chromosomes, Bacterial, Cytoskeletal Proteins, DNA, Bacterial, Escherichia coli metabolism, Fungal Proteins metabolism, Genetic Vectors, Molecular Sequence Data, Mutagenesis, Insertional, Prodigiosin analogs & derivatives, Prodigiosin biosynthesis, Sequence Deletion, Streptomyces metabolism, Antigens, Bacterial, Bacterial Proteins genetics, Escherichia coli genetics, Escherichia coli Proteins, Fungal Proteins genetics, Genes, Bacterial, Repressor Proteins, Saccharomyces cerevisiae Proteins, Streptomyces genetics
- Abstract
A Streptomyces coelicolor gene, called spaA, homologous to the stationary phase regulatory gene rspA of Escherichia coli [Huisman and Kolter (1994) Science 265, 537-539], was cloned using the Streptomyces ambofaciens rspA homologue spa2 [Schneider et al. (1993) J. Gen. Microbiol. 139, 2559-2567] as a probe. Considerable differences in sequence and in genetic context were detected between spa2 of S. ambofaciens and spaA of S. coelicolor. A cloned internal fragment of spaA was used to direct integration of a phage vector into the spaA gene. The disruption caused delayed antibiotic production (undecylprodigiosin and actinorhodin) and led on further incubation to increased actinorhodin production at high, but not low, cell density. This phenotype was apparent only on the nutritionally poorest of three media tested. The attempted use of an integrating plasmid-based system for gene replacement of spaA gave rise to extensive deletions of adjacent chromosomal DNA.
- Published
- 1996
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