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1. Selectable cassettes for simplified construction of yeast gene disruption vectors.

Author

Earley MC and Crouse GF

Subjects
Cloning, Molecular methods, Kanamycin Resistance, Molecular Sequence Data, Recombination, Genetic, Genetic Vectors, Mutagenesis, Insertional methods, Saccharomyces cerevisiae genetics
Abstract

Cassettes based on a hisG-URA3-hisG insert have been modified by the addition of an KmR-encoding gene and flanking polylinker sites, greatly simplifying construction of gene disruption vectors in Escherichia coli. After gene disruption in yeast, URA3 can then be excised by recombination between the hisG repeats flanking the gene, permitting reuse of the URA3 marker.

Published
1996
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