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1. A vector, pSHT, for the expression and secretion of protein domains in mammalian cells.

Author

Madison EL and Bird P

Subjects
Animals, Base Sequence, DNA, Mammals, Molecular Sequence Data, Promoter Regions, Genetic, Recombinant Proteins metabolism, T-Phages genetics, Genetic Vectors, Plasmids, Recombinant Proteins genetics
Abstract

A phagemid (pSHT) containing the pUC and M13 ori sequences was constructed to facilitate the expression of partial cDNAs or of sequences encoding mammalian membrane- and secretory-protein domains. It provides a start codon and signal sequence flanked upstream by the simian virus 40 and bacteriophage T7 promoters and downstream by cloning sites, stop codons in all three frames, splicing and polyadenylation signals.

Published
1992
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