1. DC-SIGN mediated antigen-targeting using glycan-modified liposomes: formulation considerations.
- Author
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Joshi MD, Unger WW, van Beelen AJ, Bruijns SC, Litjens M, van Bloois L, Kalay H, van Kooyk Y, and Storm G
- Subjects
- Antigen-Presenting Cells immunology, Cell Adhesion Molecules immunology, Dendritic Cells immunology, Drug Delivery Systems, Feasibility Studies, Lectins, C-Type immunology, Liposomes, Polyethylene Glycols chemistry, Polysaccharides chemistry, Receptors, Cell Surface immunology, Antigen-Presenting Cells metabolism, Cell Adhesion Molecules metabolism, Lectins, C-Type metabolism, Major Histocompatibility Complex immunology, Receptors, Cell Surface metabolism
- Abstract
Dendritic cells (DCs) are key antigen presenting cells that have the unique ability to present antigens on MHC molecules, which can lead to either priming or suppression of T cell mediated immune responses. C-type lectin receptors expressed by DCs are involved in antigen uptake and presentation through recognition of carbohydrate structures on antigens. Here we have explored the feasibility of modification of liposomes with glycans for targeting purposes to boost immune responses. The potential of targeting glycoliposomal constructs to the C-type lectin DC-SIGN on DCs was studied using either PEGylated or non-PEGylated liposomes. Our data demonstrate that formulation of the glycoliposomes as PEGylated negatively affected their potential to target to DCs., (Copyright © 2011 Elsevier B.V. All rights reserved.)
- Published
- 2011
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