1. Formulation development of nanostructured lipid carrier-based nanogels encapsulating tacrolimus for sustained therapy of psoriasis.
- Author
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Kakade P, Patravale V, Patil A, and Disouza J
- Subjects
- Animals, Mice, Delayed-Action Preparations, Particle Size, Nanostructures chemistry, Nanostructures administration & dosage, Nanoparticles chemistry, Immunosuppressive Agents administration & dosage, Immunosuppressive Agents chemistry, Male, Imiquimod administration & dosage, Fibroblasts drug effects, Chemistry, Pharmaceutical methods, Gels, Polyethylene Glycols chemistry, Polyethylene Glycols administration & dosage, Polyethyleneimine, Psoriasis drug therapy, Drug Carriers chemistry, Drug Liberation, Lipids chemistry, Lipids administration & dosage, Tacrolimus administration & dosage, Tacrolimus chemistry, Tacrolimus pharmacokinetics, Nanogels chemistry
- Abstract
The goal of this study was to formulate tacrolimus nanogel based on nanostructured lipid carrier (NLC) in order to improve the efficacy, aesthetic, and patient compliance for the treatment of psoriasis. The microemulsion method was used to create phase diagrams and NLCs were prepared using points obtained from the microemulsion region and characterized. The gelling agent carbopol was used to develop an NLC-based nanogel. The pH, drug assay, viscosity, spreadability, and in vitro release of the nanogel, were evaluated. Ex vivo cytotoxicity of the formulation was assessed in murine fibroblast cells. Oxazolone and imiquimod models of psoriasis were used to assess the effectiveness of the nanogel. The NLCs exhibited a submicron particle size of 320 ± 10 nm, a low polydispersity index (<0.3), and a zeta potential of -19.4 mV. Morphological analysis revealed spherical nanoparticles with an encapsulation efficiency of 60 ± 3 %. The nanogel maintained a pH of 6.0 ± 0.5 and possessed a remarkable drug content of 99.73 ± 1.4 %. It exhibited pseudoplastic flow behaviour, ensuring easy spreadability, and demonstrated sustained drug release exceeding 90 % over a 24-hr period. Ex vivo cytotoxicity assessments revealed that the nanogel was safe because no cell death was induced. Nanogel resolved psoriatic blisters, was non-irritating and improved skin elasticity. The favorable properties, safety profile, and remarkable efficacy show the potential of the nanogel as a patient-friendly and effective therapeutic option for psoriasis treatment., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)
- Published
- 2024
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