1. Examination of real-time PCR for HIV-1 RNA and DNA quantitation in patients infected with HIV-1 BF intersubtype recombinant variants.
- Author
-
Schvachsa N, Turk G, Burgard M, Dilernia D, Carobene M, Pippo M, Gómez-Carrillo M, Rouzioux C, and Salomon H
- Subjects
- DNA, Viral analysis, Genetic Variation, HIV Infections virology, HIV Long Terminal Repeat genetics, HIV-1 classification, HIV-1 genetics, Humans, Nucleic Acid Amplification Techniques, Viral Load, HIV Infections diagnosis, HIV-1 isolation & purification, Polymerase Chain Reaction methods, RNA, Viral analysis, Recombination, Genetic
- Abstract
The impact of HIV-1 genetic diversity on the performance of laboratory testing is an issue that has to be monitored continuously. An "in-house" real-time PCR assay was developed by the Agence Nationale de Recherche sur le SIDA (ANRS) in France for viral load (VL) quantitation based on the amplification of the HIV-1 long terminal repeat (LTR) region. This technology has not been used in Argentina yet and considering the HIV-1 diversity in the country, a comparative analysis of this assay was undertaken versus the Versant HIV-1 RNA 3.0 Assay (b-DNA). The performance was assessed on 30 drug-naïve HIV-1 infected patients who were characterized previously by phylogenetic analysis of the pol and vpu gene. The results showed that there is a significant linear correlation between values of transformed viral load logarithms measured by both, bDNA and real-time PCR assay and that this assay can be used to quantify viral load in samples from BF-infected patients with the same accuracy and reliability as for B subtype samples. The use of "in-house" real-time PCR to measure DNA in PBMCs correlated strongly with the HIV-1 RNA levels in all specimens.
- Published
- 2007
- Full Text
- View/download PDF