1. All-in-one hydrogel patches with sprayed bFGF-loaded GelMA microspheres for infected wound healing studies.
- Author
-
Jin W, Shen S, Xu X, Xie X, Zhou X, Su X, Wu L, Wang S, Zhang L, Chen B, and Yang F
- Subjects
- Animals, Mice, Male, Dopamine administration & dosage, Dopamine chemistry, Dopamine pharmacology, Hyaluronic Acid chemistry, Hyaluronic Acid administration & dosage, Hyaluronic Acid pharmacology, RAW 264.7 Cells, Cell Movement drug effects, Wound Infection drug therapy, Wound Healing drug effects, Fibroblast Growth Factor 2 administration & dosage, Fibroblast Growth Factor 2 chemistry, Fibroblast Growth Factor 2 pharmacology, Microspheres, Gelatin chemistry, Staphylococcus aureus drug effects, Anti-Bacterial Agents administration & dosage, Anti-Bacterial Agents pharmacology, Anti-Bacterial Agents chemistry, Hydrogels chemistry, Hydrogels administration & dosage, Chitosan chemistry, Chitosan administration & dosage, Antioxidants administration & dosage, Antioxidants pharmacology, Antioxidants chemistry, Methacrylates chemistry, Methacrylates administration & dosage, Anti-Inflammatory Agents administration & dosage, Anti-Inflammatory Agents pharmacology, Anti-Inflammatory Agents chemistry
- Abstract
The current wound healing process faces numerous challenges such as bacterial infection, inflammation and oxidative stress. However, wound dressings used to promote wound healing, are not well suited to meet the clinical needs. Hyaluronic acid (HA) not only has excellent water absorption and good biocompatibility but facilitates cell function and tissue regeneration. Dopamine, on the other hand, increases the overall viscosity of the hydrogel and possesses antioxidant property. Furthermore, chitosan exhibits outstanding performance in antimicrobial, anti-inflammatory and antioxidant activities. Basic fibroblast growth factor (bFGF) is conducive to cell proliferation and migration, vascular regeneration and wound healing. Hence, we designed an all-in-one hydrogel patch containing dopamine and chitosan framed by hyaluronic acid (HDC) with sprayed gelatin methacryloyl (GelMA) microspheres loaded with bFGF (HDC-bFGF). The hydrogel patch exhibits excellent adhesive, anti-inflammatory, antioxidant and antibacterial properties. In vitro experiments, the HDC-bFGF hydrogel patch not only showed significant inhibitory effect on RAW cell inflammation and Staphylococcus aureus (S. aureus) growth but also effectively scavenged free radicals, in addition to promoting the migration of 3 T3 cells. In the mice acute infected wound model, the HDC-bFGF hydrogel patch adhered to the wound surface greatly accelerated the healing process via its anti-inflammatory and antioxidant activities, bacterial inhibition and pro-vascularization effects. Therefore, the multifunctional HDC-bFGF hydrogel patch holds great promise for clinical application., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF