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Alkaline residuals, such as wood ash and lime mud generated from pulp and paper mills, could be recycled as liming agents in sugar maple (Acer saccharum Marsh.) forests affected by soil acidification. The objectives of this study were (1) to evaluate soil chemistry, in particular soil acidity, after the application of three alkaline residuals from the pulp and paper industry, and (2) to determine if these alkaline residuals altered soil greenhouse gas (GHG) emissions as a result of the change in soil pH or due to their chemical composition. Soil properties and GHG fluxes were monitored for two years after alkaline residuals were applied to six forest sites dominated by sugar maple in southeastern Quebec, Canada. Each site received six treatments: wood ash applied at 5, 10 and 20 t ha -1 , lime mud (7.5 t ha -1 ), a mixture of slaker grits and green liquor sludge (7 t ha -1 ) and an unamended control. These treatments had acid-neutralizing power from 0 to 9 t ha -1 . All alkaline residuals buffered soil acidity as a function of their neutralizing power, and more neutralization occurred in the forest floor layer than in the underlying mineral soil. In the forest floor, the alkaline residual treatments significantly increased pH by more than one unit, nearly doubled the base saturation, and reduced exchangeable acidity, Al and Fe concentrations compared to control plots. The CO 2 and N 2 O fluxes were lower after application of alkaline residuals, and this was related to the soil pH increase and the type of alkaline residual applied. Lime mud was more effective at reducing GHG fluxes than other alkaline residuals. We conclude that these alkaline residuals can effectively counteract soil acidity in sugar maple forests without increasing soil GHG emissions, at least in the short term., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Globally, the production of vast volumes of municipal solid waste impacts public health and the climate. Greening the solid waste sector could contribute to the achievement of the Sustainable Development Goals (SDGs). Therefore, this study assessed how the potential electricity and environmental benefits of recycling paper and plastic wastes could contribute to the achievement of the SDGs. The study used model equations methods to estimate the electricity consumption, diesel consumption, and greenhouse gas (GHG) emissions saved due to recycling. The key findings show that the 132.4 thousand toe/day of electricity saved globally in 2012 soared to 182.3 thousand toe/day in 2025. In 2012 and 2025, an average of 63.6% of electricity was saved. Globally, recycling saved a record of 1.4 million ktons CO 2 eq/day of GHG emissions in 2012 and 1.9 million ktons CO 2 eq/day in 2025. It was further realized that the overall volume of diesel fuel saved in 2012 grew from 511,146 to 703,887 million Liters/day in 2025, representing a rise of 37.7%. The sensitivity analysis shows an increase in the waste collection rate, the fraction of paper waste, the fraction of plastic waste, and the recycling rate in 2025 will boost recyclable resources' energy and environmental benefits. The findings of this study could offer scientific guidance for the achievement of the SDGs related to solid waste recycling and management., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Microplastics (MPs) are emerging contaminants in aquatic ecosystems that can profoundly affect carbon and nitrogen cycling. However, the impact mechanisms of MPs on sedimentary greenhouse gas (GHG) emissions at distinct altitudes remain poorly elucidated. Here, we investigated the effects of polyvinyl chloride (PVC) and polylactic acid (PLA) on sedimentary CO 2 , CH 4 , and N 2 O emissions at distinct altitudes of the Yellow River. PVC increased the relative abundance of denitrifiers (e.g., Xanthobacteriaceae, Rhodocyclaceae) to promote N 2 O emissions, whereas PLA reduced the abundance of AOA gene and denitrifiers (e.g., Pseudomonadaceae, Sphingomonadaceae), impeding N 2 O emissions. Both PVC and PLA stimulated the growth of microbes (Saprospiraceae, Aquabacterium, and Desulfuromonadia) associated with complex organics degradation, leading to increased CO 2 emissions. Notably, the concurrent inhibition of PLA on mcrA and pmoA genes led to its minimal impact on CH 4 emissions. High-altitude MQ sediments, characterized by abundant substrate and a higher abundance of functional genes (AOA, AOB, nirK, mcrA), demonstrated higher GHG emissions. Conversely, lower microbial diversity rendered the low-altitude LJ microbial community more susceptible to PVC, leading to a more significant promotion on GHG emissions. This study unequivocally confirms that MPs exacerbate GHG emissions via microbiome-mediated mechanisms, providing a robust theoretical foundation for microplastic control to mitigate global warming., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

Rice production is a primary contributor to global greenhouse gas emissions, with unclear pathways towards carbon neutrality. Here, through a comprehensive assessment of direct greenhouse gas (GHG) emission using DNDC model and indirect GHG emission using emission factor methods, we estimated the annual crop yield, GHG emission amount and intensity, and economic benefits of different cropping patterns in the climate-sensitive regions of rice production in China. Through the expansion of single-rice and cropping pattern change from the wheat-rice to wheat-rice-rice in the climate-sensitive regions of single and triple-cropping cultivations, the total grain yield increased by 4.4 % and 4.5 % compared with the current national grain production, the GHG emission would increase by 2.4 % and 5.4 % of the current national GHG emissions from rice and wheat production, the net economic benefits could increase 0.9 % and decrease 2.0 % of the national output value of rice and wheat production. The study takes the entire-life cycle of crop growth as the principal line, and could provide a valuable reference for the regulation of the cropping pattern and the formulation of carbon reduction policies in the climate-sensitive region., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

Agriculture activity contributes to greenhouse gas (GHG) emissions through its utilization of land, water, and energy for food production. Hence, the interactions between land, water, and GHG emissions in agricultural production need to be comprehensively studied. The study aimed to assess the Land-Water-GHG-Food Nexus Index (LWGFNI) of rice cultivation across various land suitability classes in Central Thailand and determining the physical, socio-economic, and policy factors that can influence farmers' decisions to choose for cultivating rice instead of shifting to other crops. The results indicated that the highest LWGFNI score was 0.69 for the rice grown in the moderate suitability land class which revealed a lower use of land and water resources as well as GHG emissions compared to other levels of land suitability. The LWGFNI scores of major rice cultivation were greater compared to the second rice in all four-land suitability. The use of fertilizers had a crucial role in enhancing productivity levels and was a significant factor in the generation of GHG emissions. Hence, improving effective production should consider the appropriate use of fertilizer. The physical, socio-economic, and policy-related aspects that significantly influenced farmers' decisions on cultivation of rice included topography, water resources, inherited professions, price guarantee, and knowledge/training factors. The methodology used and results obtained can help policy makers to plan the use of water and land resources efficiently and appropriately with local resources based on land suitability class. The assessment results revealed the GHG hotspots and the strategies to mitigate GHG emissions associated with rice cultivation., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Microplastics pollution and climate change are primarily investigated in isolation, despite their joint threat to the environment. Greenhouse gases (GHGs) are emitted during: the production of plastic and rubber, the use and degradation of plastic, and after contamination of environment. This is the first meta-analysis to assess underlying causal relationships and the influence of likely mediators. We included 60 peer-reviewed empirical studies; estimating GHGs emissions effect size and global warming potential (GWP), according to key microplastics properties and soil conditions. We investigated interrelationships with microbe functional gene expression. Overall, microplastics contamination was associated with increased GHGs emissions, with the strongest effect (60%) on CH 4 emissions. Polylactic-acid caused 32% higher CO 2 emissions, but only 1% of total GWP. Phenol-formaldehyde had the greatest (175%) GWP via 182% increased N 2 O emissions. Only polystyrene resulted in reduced GWP by 50%, due to N 2 O mitigation. Polyethylene caused the maximum (60%) CH 4 emissions. Shapes of microplastics differed in GWP: fiber had the greatest GWP (66%) whereas beads reduced GWP by 53%. Films substantially increased emissions of all GHGs: 14% CO 2 , 10% N 2 O and 60% CH 4 . Larger-sized microplastics had higher GWP (125%) due to their 9% CO 2 and 63% N 2 O emissions. GWP rose sharply if soil microplastics content exceeded 0.5%. Higher CO 2 emissions, ranging from 4% to 20%, arose from soil which was either fine, saturated or had high-carbon content. Higher N 2 O emissions, ranging from 10% to 95%, arose from soils that had either medium texture, saturated water content or low-carbon content. Both CO 2 and N 2 O emissions were 43%-56% higher from soils with neutral pH. We conclude that microplastics contamination can cause raised GHGs emissions, posing a risk of exacerbating climate-change. We show clear links between GHGs emissions, microplastics properties, soil characteristics and soil microbe functional gene expression. Further research is needed regarding underlying mechanisms and processes., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Constructed wetlands for wastewater treatment pose challenges related to long-term operational efficiency and greenhouse gas emissions on a global scale. This study investigated the impact of adding peat, humic acid, and biochar into the substrates of constructed wetlands and focused on Cr, and Ni removal, greenhouse gas emissions, and microbial communities in constructed wetlands. Biochar addition treatment achieved the highest removal efficiencies for total Cr (99.96%), Cr (VI) (100%), and total Ni (91.04%). Humic acid and biochar addition both significantly increased the heavy metal content in wetland plant Leersia hexandra and substrates of constructed wetlands. Further analysis of microbial community proportions by high-throughput sequencing revealed that biochar and humic acid treatments enhanced Cr and Ni removal efficiency by increasing the abundance of Bacteroidetes, Geobacter and Ascomycota. Humic acid addition treatment reduced CO 2 emissions by decreasing the abundance of Bacteroidetes and increasing that of Basidiomycota. Peat treatment decreased CH 4 emissions by reducing the abundance of the Bacteroidetes. Biochar treatment increased the abundance of the Firmicutes, Bacteroidetes, Proteobacteria as well as Basidiomycota, resulting in reduced N 2 O emissions. Biochar and humic acid treatments efficiently removed heavy metals from wastewater and mitigated greenhouse gas emissions in constructed wetlands by modifying the microbial communities., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Ensuring agricultural security and preserving the health of wetland ecosystems are crucial concerns facing northeast China. However, the adverse effects of environmental pollution, especially nitrogen (N), caused by prolonged agricultural development on the health of marsh wetlands cannot be systematically recognized. To address this issue, an 18-year trial with four different levels of N application was carried out in a typical area of the Northeast region: 0, 6, 12, and 24 gN·m -2 ·a -1 (referred to as CK, N6, N12, and N24, respectively) to investigate changes in wetland ecological functioning. The results showed that long-term N input significantly enhanced soil N availability. High-level of N addition (N24) significantly reduced soil bacterial richness in October, while fungal diversity was significantly higher in June than in October for both control and N6 treatments. The main environmental factors affecting microorganisms in June were TN, NH 4 + , and EC, while bacterial and fungal communities were influenced by TN and Leaf Area Index (LAI), respectively, in October. It was found that the AN16S gene was significantly higher in June than in October, indicating that summer is the critical time for N removal in the wetland. N addition significantly reduced the abundance of the NIFH gene and decreased the N fixation potential of the wetland. In June, low and medium levels of N inputs promoted denitrification processes in the wetland and elevated the wetland N 2 O emission potential. The abundance of NARG, NIRK, and NOSZ genes decreased significantly in October compared to June, indicating a decrease in the wetland N 2 O emission potential. Additionally, it was observed that soil methanotrophs were positively affected by NH 4 + and TN in October, thereby reducing the wetland CH 4 emission potential. Our research provides a systematic understanding of the impact of agricultural N pollution on marsh wetlands, which can inform strategies to protect wetland health., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Diet shift is an opportunity to mitigate the impacts of food systems, which are responsible for about a third of greenhouse gas (GHG) emissions globally and exert various environmental pressures on ecosystems. This study evaluates the mitigation potential of both global and local environmental impacts through dietary changes within the Brazilian context. Furthermore, the study aims to identify the potential benefits and trade-offs that may arise from these dietary transitions, thus providing a comprehensive analysis of the overall environmental implications. To this end, a life cycle assessment (LCA) was performed to evaluate the environmental impacts of a conventional diet in Brazil and seven alternatives, namely adjusted-EAT-Lancet, pescatarian, vegetarian, entomophagic (insect-based food), mycoprotein (microbial-based food), and synthetic (cell-based food) diets. Results indicate a substantial mitigation potential for GHG emissions (4-9 kg CO 2 e/cap/day) (39 % to 86 %) and land use (4-9 m 2 /cap/day) (38 % to 82 %) through a diet shift from a conventional diet to any of the seven alternative diets. However, certain trade-offs exist. A diet shift demonstrates no mitigation potential of soil acidification, and opportunities to reduce water eutrophication (0.02-0.2 g Pe/cap/day) (2 % to 24 %) and water consumption (0.2-0.5 m 3 /cap/day) (7 % to 14 %) were only found by completely substituting animal products for insect-based food, microbial-based food, and cell-based food. This study highlights the considerable potential of dietary changes to mitigate global environmental impacts associated with food systems. By revealing opportunities and challenges, this study supports science-based decision-making and guides efforts toward sustainable and environmentally friendly food consumption patterns., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Tamar Bakman reports financial support was provided by Coordination of Higher Education Personnel Improvement. Tamar Bakman reports financial support was provided by Carlos Chagas Filho Foundation for Research Support of Rio de Janeiro State. Tamar Bakman reports financial support was provided by National Council for Scientific and Technological Development. Joana Portugal-Pereira reports financial support was provided by National Council for Scientific and Technological Development. Joana Portugal-Pereira reports financial support was provided by Foundation for Science and Technology. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Soil adsorption, which could be seen as a crucial ecosystem service, plays a pivotal role in regulating environmental quality and climate dynamics. However, despite its significance, it is often undervalued within the realms of research and policy frameworks. This article delves into the multifaceted aspects of soil adsorption, incorporating insights from chemistry and material science, ecological perspectives, and recent advancements in the field. In exploring soil components and their adsorption capacities, the review highlights how organic and inorganic constituents orchestrate soil's aptitude for pollutant mitigation and nutrient retention/release. Innovative materials and technologies such as biochar are evaluated for their efficacy in enhancing these natural processes, drawing a link with the sustainability of agricultural systems. The symbiosis between soil microbial diversity and adsorption mechanisms is examined, emphasizing the potential for leveraging this interaction to bolster soil health and resilience. The impact of soil adsorption on global nutrient cycles and water quality underscores the environmental implications, portraying it as a sentinel in the face of escalating anthropogenic activities. The complex interplay between soil adsorption mechanisms and climate change is elaborated, identifying research gaps and advocating for future investigations to elucidate the dynamics underpinning this relation. Policy and socioeconomic aspects form a crucial counterpart to the scientific discourse, with the review assessing how effective governance, incentivization, and community engagement are essential for translating soil adsorption's functionality into tangible climate change mitigation and sustainable land-use strategies. Integrating these diverse but interconnected strata, the article presents a comprehensive overview that not only charts the current state of soil adsorption research but also casts a vision for its future trajectory. It calls for an integrated approach combining scientific inquiry, technological innovation, and proactive policy to leverage soil adsorption's full potential to address environmental challenges and catalyze a transition towards a more sustainable and resilient future., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

In India, majority of the generated municipal solid waste (MSW) was dumped in poorly managed landfills and dumpsites over the past decades and is an environmental and health hazard. Landfill mining is a promising solution to reclaim these sites along with the recovery of resources (materials and energy). During landfill mining operations, the combustible fraction is one of the major components recovered and needs proper management for maximizing resource recovery. For the identification of appropriate resource recovery options, knowledge of the physicochemical characteristics is required. The present study aims to assess the depth-wise change in the composition of legacy waste and the physicochemical characteristics of the combustible fraction. Furthermore, a material flow analysis considering the incineration of combustible fraction was performed to estimate the energy generation potential and the associated greenhouse gas (GHG) emissions. The results of the compositional analysis of dry legacy waste revealed that the fine fraction (<4 mm soil-like material) was dominating with a share of 36%. The depth-wise analysis showed a decrease in the calorific value with increasing landfill depth, while no specific trend was observed for the other parameters analyzed, including proximate and ultimate analysis, and chlorine content. The material flow analysis performed for 100 tonnes of wet legacy waste indicated that 52 tonnes of waste is combustible fraction. The GHG emissions through incineration of one tonne of dry combustible fraction would be 1389 kg CO 2 -eq, with 1125 kWh of electrical energy generation potential., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Tire wear particles (TWPs), as predominant microplastics (MPs) in road runoff, can be captured and retained by bioretention systems (BRS). This study aimed to investigate the effect of TWPs accumulation on nitrogen processes, focusing on soil characteristics, microbial community, and functional genes. Two groups of lab-scale bioretention columns containing TWPs (0 and 100 mg g -1 ) were established. The removal efficiencies of NH 4 + -N and TN in BRS significantly decreased by 7.60%-24.79% and 1.98%-11.09%, respectively, during the 101 days of TWPs exposure. Interestingly, the emission fluxes of N 2 O and CO 2 were significantly decreased, while the emission flux of CH 4 was substantially increased. Furthermore, prolonged TWPs exposure significantly influenced the contents of soil organic matter (increased by 27.07%) and NH 4 + -N (decreased by 42.15%) in the planting layer. TWPs exposure also significantly increased dehydrogenase activity and substrate-induced respiration rate, thereby promoting microbial metabolism. Microbial sequencing results revealed that TWPs decreased the relative abundance of nitrifying bacteria (Nitrospira and Nitrosomonas) and denitrifying bacteria (Dechloromonas and Thauera), reducing the nitrification rate by 42.24%. PICRUSt2 analysis further indicated that TWPs changed the relative abundance of functional genes related to nitrogen and enzyme-coding genes., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Greenhouse gas (GHG) fluxes from peatland soils are relatively well studied, whereas tree stem fluxes have received far less attention. Simultaneous year-long measurements of soil and tree stem GHG fluxes in northern peatland forests are scarce, as previous studies have primarily focused on the growing season. We determined the seasonal dynamics of tree stem and soil CH 4 , N 2 O and CO 2 fluxes in a hemiboreal drained peatland forest. Gas samples for flux calculations were manually collected from chambers at different heights on Downy Birch (Betula pubescens) and Norway Spruce (Picea abies) trees (November 2020-December 2021) and analysed using gas chromatography. Environmental parameters were measured simultaneously with fluxes and xylem sap flow was recorded during the growing season. Birch stems played a greater role in the annual GHG dynamics than spruce stems. Birch stems were net annual CH 4 , N 2 O and CO 2 sources, while spruce stems constituted a CH 4 and CO 2 source but a N 2 O sink. Soil was a net CO 2 and N 2 O source, but a sink of CH 4 . Temporal dynamics of stem CH 4 and N 2 O fluxes were driven by isolated emissions' peaks that contributed significantly to net annual fluxes. Stem CO 2 efflux followed a seasonal trend coinciding with tree growth phenology. Stem CH 4 dynamics were significantly affected by the changes between wetter and drier periods, while N 2 O was more influenced by short-term changes in soil hydrologic conditions. We showed that CH 4 emitted from tree stems during the wetter period can offset nearly half of the soil sink capacity. We presented for the first time the relationship between tree stem GHG fluxes and sap flow in a peatland forest. The net CH 4 flux was likely an aggregate of soil-derived and stem-produced CH 4 . A dominating soil source was more evident for stem N 2 O fluxes., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Agriculture is a leading sector in international initiatives to mitigate climate change and promote sustainability. This article exhaustively examines the removals and emissions of greenhouse gases (GHGs) in the agriculture industry. It also investigates an extensive range of GHG sources, including rice cultivation, enteric fermentation in livestock, and synthetic fertilisers and manure management. This research reveals the complex array of obstacles that are faced in the pursuit of reducing emissions and also investigates novel approaches to tackling them. This encompasses the implementation of monitoring systems powered by artificial intelligence, which have the capacity to fundamentally transform initiatives aimed at reducing emissions. Carbon capture technologies, another area investigated in this study, exhibit potential in further reducing GHGs. Sophisticated technologies, such as precision agriculture and the integration of renewable energy sources, can concurrently mitigate emissions and augment agricultural output. Conservation agriculture and agroforestry, among other sustainable agricultural practices, have the potential to facilitate emission reduction and enhance environmental stewardship. The paper emphasises the significance of financial incentives and policy frameworks that are conducive to the adoption of sustainable technologies and practices. This exhaustive evaluation provides a strategic plan for the agriculture industry to become more environmentally conscious and sustainable. Agriculture can significantly contribute to climate change mitigation and the promotion of a sustainable future by adopting a comprehensive approach that incorporates policy changes, technological advancements, and technological innovations., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Constructed wetlands (CWs) are pivotal for wastewater treatment due to their high efficiency and numerous advantages. The impact of plant species and diversity on greenhouse gas (GHG) emissions from CWs requires a more comprehensive evaluation. Moreover, controversial perspectives persist about whether CWs function as carbon sinks or sources. In this study, horizontal subsurface flow (HSSF) CWs vegetated with Cyperus alternifolius, Typhae latifolia, Acorus calamus, and the mixture of these three species were constructed to evaluate pollutant removal efficiencies and GHG emissions, and estimate carbon budgets. Polyculture CWs can stably remove COD (86.79 %), NH 4 + -N (97.41 %), NO 3 - -N (98.55 %), and TP (98.48 %). They also mitigated global warming potential (GWP) by suppressing N 2 O emissions compared with monoculture CWs. The highest abundance of the Pseudogulbenkiania genus, crucial for denitrification, was observed in polyculture CWs, indicating that denitrification dominated in nitrogen removal. While the highest nosZ copy numbers were observed in CWs vegetated with Cyperus alternifolius, suggesting its facilitation of denitrification-related microbes. Selecting Cyperus alternifolius to increase species diversity is proposed for simultaneously maintaining the water purification capacity and reducing GHG emissions. Carbon budget estimations revealed that all four types of HSSF CWs were carbon sinks after six months of operation, with carbon accumulation capacity of 4.90 ± 1.50 (Cyperus alternifolius), 3.31 ± 2.01 (Typhae latifola), 1.78 ± 1.30 (Acorus calamus), and 2.12 ± 0.88 (polyculture) kg C/m 2 /yr. This study implies that under these operation conditions, CWs function as carbon sinks rather than sources, aligning with carbon peak and neutrality objectives and presenting significant potential for carbon reduction efforts., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Mangroves forests may be important sinks of carbon in coastal areas but upon their death, these forests may become net sources of carbon dioxide (CO 2 ) and methane (CH 4 ) to the atmosphere. Here we assessed the spatial and temporal variability in soil CO 2 and CH 4 fluxes from dead mangrove forests and paired intact sites in SE-Brazil. Our findings demonstrated that during warmer and drier conditions, CO 2 soil flux was 183 % higher in live mangrove forests when compared to the dead mangrove forests. Soil CH 4 emissions in live forests were > 1.4-fold higher than the global mangrove average. During the wet season, soil GHG emissions dropped significantly at all sites. During warmer conditions, mangroves were net sources of GHG, with a potential warming effect (GWP100) of 32.9 ± 10.2 (±SE) Mg CO 2 e ha -1 y -1 . Overall, we found that dead mangroves did not release great amounts of GHG after three years of forest loss., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Although anthropogenic activities are the primary drivers of increased greenhouse gas (GHG) emissions, it is crucial to acknowledge that wetlands are a significant source of these gases. Brazil's Pantanal, the largest tropical inland wetland, includes numerous lacustrine systems with freshwater and soda lakes. This study focuses on soda lakes to explore potential biogeochemical cycling and the contribution of biogenic GHG emissions from the water column, particularly methane. Both seasonal variations and the eutrophic status of each examined lake significantly influenced GHG emissions. Eutrophic turbid lakes (ET) showed remarkable methane emissions, likely due to cyanobacterial blooms. The decomposition of cyanobacterial cells, along with the influx of organic carbon through photosynthesis, accelerated the degradation of high organic matter content in the water column by the heterotrophic community. This process released byproducts that were subsequently metabolized in the sediment leading to methane production, more pronounced during periods of increased drought. In contrast, oligotrophic turbid lakes (OT) avoided methane emissions due to high sulfate levels in the water, though they did emit CO 2 and N 2 O. Clear vegetated oligotrophic turbid lakes (CVO) also emitted methane, possibly from organic matter input during plant detritus decomposition, albeit at lower levels than ET. Over the years, a concerning trend has emerged in the Nhecolândia subregion of Brazil's Pantanal, where the prevalence of lakes with cyanobacterial blooms is increasing. This indicates the potential for these areas to become significant GHG emitters in the future. The study highlights the critical role of microbial communities in regulating GHG emissions in soda lakes, emphasizing their broader implications for global GHG inventories. Thus, it advocates for sustained research efforts and conservation initiatives in this environmentally critical habitat., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Although benthic microbial community offers crucial insights into ecosystem services, they are underestimated for coastal sediment monitoring. Sepetiba Bay (SB) in Rio de Janeiro, Brazil, holds long-term metal pollution. Currently, SB pollution is majorly driven by domestic effluents discharge. Here, functional prediction analysis inferred from 16S rRNA gene metabarcoding data reveals the energy metabolism profiles of benthic microbial assemblages along the metal pollution gradient. Methanogenesis, denitrification, and N 2 fixation emerge as dominant pathways in the eutrophic/polluted internal sector (Spearman; p < 0.05). These metabolisms act in the natural attenuation of sedimentary pollutants. The methane (CH 4 ) emission (mcr genes) potential was found more abundant in the internal sector, while the external sector exhibited higher CH 4 consumption (pmo + mmo genes) potential. Methanofastidiosales and Exiguobacterium, possibly involved in CH 4 emission and associated with CH 4 consumers respectively, are the main taxa detected in SB. Furthermore, SB exhibits higher nitrous oxide (N 2 O) emission potential since the norB/C gene proportions surpass nosZ up to 4 times. Blastopirellula was identified as the main responsible for N 2 O emissions. This study reveals fundamental contributions of the prokaryotic community to functions involved in greenhouse gas emissions, unveiling their possible use as sentinels for ecosystem monitoring., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Livestock and poultry products are an essential human food source. However, the rapid development of the livestock sector (LS) has caused it to become a significant source of greenhouse gas (GHG) emissions. Consequently, investigating the spatio-temporal characteristics and evolution of GHG emissions is crucial to facilitate the green development of the LS and achieve "peak carbon and carbon neutrality". This study combined life cycle assessment (LCA) with the IPCC Tier II method to construct a novel GHG emissions inventory. The GHG emissions of 31 provinces in China from 2000 to 2021 were calculated, and their spatio-temporal characteristics were revealed. Then, the stochastic impacts by regression on population, affluence, and technology (STIRPAT) model was used to identify the main driving factors of GHG emissions in six regions of China and explore the emission reduction potential. The results showed that GHG emissions increased and then decreased from 2000 to 2021, following a gradual and steady trend. The peak of 628.55 Mt CO 2 -eq was reached in 2006. The main GHG-producing segments were enteric fermentation, slaughtering and processing, and manure management, accounting for 45.39 %, 26.34 %, and 23.08 % of total GHG emissions, respectively. Overall, the center of gravity of GHG emissions in China migrated northward, with spatial aggregation observed since 2016. The high emission intensity regions were mainly located west of the "Hu Huanyong line". Economic efficiency and emissions intensity were the main drivers of GHG emissions. Under the baseline scenario, GHG emissions are not projected to peak until 2050. Therefore, urgent action is needed to promote the low-carbon green development of the LS in China. The results can serve as scientific references for the macro-prevention and control of GHG emissions, aiding strategic decision-making. Additionally, they can provide new ideas for GHG accounting in China and other countries around the world., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

The drainage of peatlands followed by land use conversion significantly impacts on the fluxes of green-house gases (GHGs, i.e. CO 2 , CH 4 , and N 2 O) to and from the atmosphere, driven by changes in soil properties and microbial communities. In this study, we compared saturated peatlands with drained ones used for sheep grazing or cultivated, which are common in South-West Iceland. These areas exhibit different degrees of soil saturation and nitrogen (N) content, reflecting the anthropic pressure gradient. We aimed at covering knowledge gaps about lack of estimates on N 2 O fluxes and drainage, by assessing the emissions of GHGs, and the impact of land conversion on these emissions. Moreover, we investigated soil microbial community functional diversity, and its connection with processes contributing to GHGs emission. GHGs emissions differed between saturated and drained peatlands, with increased soil respiration rates (CO 2 emissions) and N mineralization (N 2 O), consistent with the trend of anthropogenic pressure. Drainage drastically reduced methane (CH 4 ) emissions but increased CO 2 emissions, resulting in a higher global warming potential (GWP). Cultivation, involving occasional tillage and fertilization, further increased N 2 O emissions, mediated by higher N availability and conditions favorable to nitrification. Functional genes mirrored the overall trend, showing a shift from prevalent methanogenic archaea (mcrA) in saturated peatlands to nitrifiers (amoA) in drained-cultivated areas. Environmental variables and nutrient content were critical factors affecting community composition in both environments, which overall affected the GHGs emissions and the relative contribution of the three gases., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Selecting an appropriate electron donor to enhance nitrogen removal for treating low C/N wastewater in ecological floating beds (EFBs) is controversy. In this study, a systematic and comprehensive evaluation of sodium acetate (EFB-C), sodium thiosulfate (EFB-S) and iron scraps (EFB-Fe) was performed in a 2-year experiment on long-term viability including nitrogen removal and greenhouse gas emissions associated with key molecular biological mechanisms. The results showed that EFB-C (43-85 %) and EFB-S (40-88 %) exhibited superior total nitrogen (TN) removal. Temperature and hydraulic retention time (HRT) have significant impacts on TN removal of EFB-Fe, however, it could reach 86 % under high temperature (30-35 °C) and a long HRT (3 days), and it has lowest N 2 O (0-6.2 mg m -2 d -1 ) and CH 4 (0-5.3 mg m -2 d -1 ) fluxes. Microbial network analysis revealed that the microbes changed from competing to cooperating after adding electron donors. A higher abundance of anammox genera was enriched in EFB-Fe. The Mantel's test and structural equation model provided proof of the differences, which showed that acetate and thiosulfate were similar, whereas Fe 0 was different in the nitrogen removal mechanism. Molecular biology analyses further verified that heterotrophic, autotrophic, and mixotrophic coupled with anammox were the main TN removal pathways for EFB-C, EFB-S, and EFB-Fe, respectively. These findings provide a better understanding of the biological mechanisms for selecting appropriate electron donors for treating low C/N wastewater., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Cyanobacteria blooms (CBs) caused by eutrophication pose a global concern, especially Microcystis aeruginosa (M. aeruginosa), which could release harmful microcystins (MCs). The impact of microplastics (MPs) on allelopathy in freshwater environments is not well understood. This study examined the joint effect of adding polystyrene (PS-MPs) as representative MPs and two concentrations (2 and 8 mg/L) of pyrogallol (PYR) on the allelopathy of M. aeruginosa. The results showed that the addition of PS-MPs intensified the inhibitory effect of 8 mg/L PYR on the growth and photosynthesis of M. aeruginosa. After a 7-day incubation period, the cell density decreased to 69.7 %, and the chl-a content decreased to 48 % compared to the condition without PS-MPs (p < 0.05). Although the growth and photosynthesis of toxic Microcystis decreased with the addition of PS-MPs, the addition of PS-MPs significantly resulted in a 3.49-fold increase in intracellular MCs and a 1.10-fold increase in extracellular MCs (p < 0.05). Additionally, the emission rates of greenhouse gases (GHGs) (carbon dioxide, nitrous oxide and methane) increased by 2.66, 2.23 and 2.17-fold, respectively (p < 0.05). In addition, transcriptomic analysis showed that the addition of PS-MPs led to the dysregulation of gene expression related to DNA synthesis, membrane function, enzyme activity, stimulus detection, MCs release and GHGs emissions in M. aeruginosa. PYR and PS-MPs triggered ROS-induced membrane damage and disrupted photosynthesis in algae, leading to increased MCs and GHG emissions. PS-MPs accumulation exacerbated this issue by impeding light absorption and membrane function, further heightening the release of MCs and GHGs emissions. Therefore, PS-MPs exhibited a synergistic effect with PYR in inhibiting the growth and photosynthesis of M. aeruginosa, resulting in additional risks such as MCs release and GHGs emissions. These results provide valuable insights for the ecological risk assessment and control of algae bloom in freshwater ecosystems., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Optimizing crop distribution stands as a pivotal approach to climate change adaption, enhancing crop production sustainability, and has been recognized for its immense potential in ensuring food security while minimizing environmental impacts. Here, we developed a climate-adaptive framework to optimize the distribution of staple crops (i.e., wheat, maize, and rice) to meet the multi-dimensional needs of crop production in China. The framework considers the feasibility of the multiple cropping systems (harvesting more than once on a cropland a year) and adopts a multi-dimensional approach, incorporating goals related to crop production, water consumption, and greenhouse gas (GHG) emissions. By optimizing, the total irrigated area of three crops would decrease by 7.7 % accompanied by a substantial 69.8 % increase in rain-fed areas compared to the baseline in 2010. This optimized strategy resulted in a notable 10.0 % reduction in total GHG emissions and a 13.1 % decrease in irrigation water consumption while maintaining consistent crop production levels. In 2030, maintaining the existing crop distribution and relying solely on yield growth would lead to a significant maize production shortfall of 27.0 %, highlighting a looming challenge. To address this concern, strategic adjustments were made by reducing irrigated areas for wheat, rice, and maize by 2.3 %, 12.8 %, and 6.1 %, respectively, while simultaneously augmenting rain-fed areas for wheat and maize by 120.2 % and 55.9 %, respectively. These modifications ensure that production demands for all three crops are met, while yielding a 6.9 % reduction in GHG emissions and a 15.1 % reduction in irrigation water consumption. This optimization strategy offers a promising solution to alleviate severe water scarcity issues and secure a sustainable agricultural future, effectively adapting to evolving crop production demands in China., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

River and reservoir ecosystems have been considered as hot spots for GHG (greenhouse gas) emissions while their specific hydrological and biogeochemical processes affect GHG concentrations; however, few studies integrated river-reservoir systems to identify the dominant drivers of GHG concentrations and flux changes associated with these systems. In the present study, we examined the seasonal variations in GHG concentrations in the surface water of three river-reservoir systems in the Seine Basin. The levels and seasonal variations of GHG concentrations exhibited distinct patterns among reservoirs, upstream, and downstream rivers. The concentrations of CH 4 (methane) in the reservoirs were notably higher than those observed in both upstream and downstream rivers and showed higher values in summer and autumn, which contrasted with CO 2 (carbon dioxide) concentrations, while N 2 O (nitrous oxide) concentrations did not show an obvious seasonal pattern. A high mole ratio of CH 4 /CO 2 was found in these reservoirs, with a value of 0.03 and was more than 30 and 10 times higher than that in the upstream and downstream rivers, respectively. The three river-reservoir systems were oversaturated with GHG during the study period, with the average diffusive fluxes (expressed as CO 2eq : CO 2 equivalent) of 810 ± 1098 mg CO 2eq m -2 d -1 , 9920 ± 2413 mg CO 2eq m -2 d -1 , and 7065 ± 2704 mg CO 2eq m -2 d -1 in the reservoirs, upstream and downstream rivers, respectively. CO 2 and CH 4 -CO 2 were respectively the dominant contributors to GHG diffusive fluxes in river and reservoir sections, while N 2 O contributed negligibly to GHG diffusive fluxes in the three river-reservoir systems. Our results showed that GHG concentrations and gas transfer coefficient have varying importance in driving GHG diffusive fluxes among different sections of the river-reservoir systems. In addition, our results also show the combined effect of reservoirs and upstream rivers on the water quality variables and hydrological characteristics of downstream rivers, highlighting the future need for additional investigations of GHG processes in the river-reservoir systems., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier Inc.)

Aquatic ecosystems are being increasingly polluted by microplastics (MPs), which calls for an understanding of how MPs affect microbially driven biogenic element cycling in water environments. A 28-day incubation experiment was conducted using freshwater lake water added with three polymer types of MPs (i.e., polyethylene, polypropylene, polystyrene) separately or in combination at a concentration of 1 items/L. The effects of various MPs on microbial communities and functional genes related to carbon, nitrogen, phosphorus, and sulfur cycling were analyzed using metagenomics. Results showed that Sphingomonas and Novosphingobium, which were indicator taxa (genus level) in the polyethylene treatment group, made the largest functional contribution to biogenic element cycling. Following the addition of MPs, the relative abundances of genes related to methane oxidation (e.g., hdrD, frhB, accAB) and denitrification (napABC, nirK, norB) increased. These changes were accompanied by increased relative abundances of genes involved in organic phosphorus mineralization (e.g., phoAD) and sulfate reduction (cysHIJ), as well as decreased relative abundances of genes involved in phosphate transport (phnCDE) and the SOX system. Findings of this study underscore that MPs, especially polyethylene, increase the potential of greenhouse gas emissions (CO 2 , N 2 O) and water pollution (PO 4 3- , H 2 S) in freshwater lakes at the functional gene level., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Beibei Chai reports financial support and administrative support were provided by Science Fund for Distinguished Young Scholars of Hebei Province. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier Inc.)

Mature compost can promote the transformation of organic matter (OM) and reduce the emission of polluting gases during composting, which provides a viable approach to reduce the environmental impacts of biodegradable plastics (BPs). This study investigated the impact of mature compost on polybutylene adipate terephthalate (PBAT) degradation, greenhouse gas (GHG) emission, and microbial community structure during composting under two treatments with mature compost (MC) and without (CK). Under MC, visible plastic rupture was advanced from day 14 to day 10, and a more pronounced rupture was observed at the end of composting. Compared with CK, the degradation rate of PBAT in MC was increased by 4.44 % during 21 days of composting. Thermobifida, Ureibacillus, and Bacillus, as indicator species under MC treatment, played an important role in PBAT decomposition. Mature compost reduced the total global warming potential (GWP) by 25.91 % via inhibiting the activity of bacteria related to the production of CH 4 and N 2 O. Functional Annotation of Prokaryotic Taxa (FAPROTAX) further revealed that mature compost addition increased relative abundance of bacteria related to multiple carbon (C) cycle functions such as methylotrophy, hydrocarbon degradation and cellulolysis, inhibited nitrite denitrification and denitrification, thus alleviating the emission of GHGs. Overall, mature compost, as an effective additive, exhibits great potential to simultaneously mitigate BP and GHG secondary pollution in co-composting of food waste and PBAT., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Global aquaculture production is expected to rise to meet the growing demand for food worldwide, potentially leading to increased anthropogenic greenhouse gases (GHG) emissions. As the demand for fish protein increases, so will stocking density, feeding amounts, and nitrogen loading in aquaculture ponds. However, the impact of GHG emissions and the underlying microbial processes remain poorly understood. This study investigated the GHG emission characteristics, key microbial processes, and environmental drivers underlying GHG emissions in low and high nitrogen loading aquaculture ponds (LNP and HNP). The N 2 O flux in HNP (43.1 ± 11.3 μmol m -2  d -1 ) was significantly higher than in LNP (-11.3 ± 25.1 μmol m -2  d -1 ), while the dissolved N 2 O concentration in HNP (52.8 ± 7.1 nmol L -1 ) was 150 % higher than in LNP (p < 0.01). However, the methane (CH 4 ) and carbon dioxide (CO 2 ) fluxes and concentrations showed no significant differences (p > 0.05). N 2 O replaced CH 4 as the main source of Global Warming Potential in HNP. Pond sediments acted as a sink for N 2 O but a source for CH 4 and CO 2 . The △N 2 O/(△N 2 O + △N 2 ) in HNP (0.015 ± 0.007 %) was 7.7-fold higher than in LNP (0.002 ± 0.001 %) (p < 0.05). The chemical oxygen demand to NO 2 -N ratio was the most important environmental factor explaining the variability of N 2 O fluxes. Ammonia-oxidizing bacteria driven nitrification in water was the predominant N 2 O source, while comammox-driven nitrification and nosZII-driven N 2 O reduction in water were key processes for reducing N 2 O emission in LNP but decreased in HNP. The strong CH 4 oxidization by Methylocystis and CO 2 assimilation by algae resulted in low CH 4 emissions and CO 2 sink in the aquaculture pond. The Mantel test indicated that HNP increased N 2 O fluxes mainly through altering functional genes composition in water and sediment. Our findings suggest that there is a significant underestimation of N 2 O emissions without considering the significantly increased △N 2 O/(△N 2 O + △N 2 ) caused by increased nitrogen loading., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Two main challenges which human society faces for sustainable development goals are the maintenance of food security and mitigation of greenhouse gas (GHG) emissions. Here, we examined the impacts of six fertilization treatments including unfertilized control (CK), mineral nitrogen (N, 90 kg N ha -1 ), mineral N plus 30 kg P ha -1 phosphorus (NP), NP combined with 3.75 Mg ha -1 straw (NP + Str), farmyard manure (Man, 75 Mg ha -1 ), and NP combined with manure (NP + Man) on crop productivity and carbon emissions (soil GHG emission; GHGI, yield-based GHG intensity; NGHGB, net GHG balance; carbon footprint, CF) in a maize-wheat cropping system during two years (April 2018-June 2020) in a semi-arid continental climate after 40 years of fertilization in the Northwest China. Manure and straw increased total GHG by 38-60 % compared to the mineral fertilizers alone, which was mainly due to the 49-80 % higher direct emissions of carbon dioxide (CO 2 ) rather than nitrous oxide (N 2 O). Compared to the N fertilizer alone, organic amendments and NP increased cumulative energy yield by 134-202 % but decreased GHGI by 38-55 %, indicating that organic fertilizers increased crop productivity at the cost of higher GHG emissions. When the soil organic carbon changes (ΔSOC) were accounted for in the C emission balance, manure application acted as a net C sink due to the NGHGB recorded with -123 kg CO 2 -eq ha -1  year -1 . When producing the same yield and economic benefits, the manure and straw addition decreased the CF by 59-85 % compared to N fertilization alone. Overall, the transition from mineral to organic fertilization in the semi-arid regions is a two-way independent solution to increase agricultural productivity along with the reduction of C emissions., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Dairy production systems display a wide range of greenhouse gas (GHG) emission characteristics influenced by factors like geographical location, farm size, herd composition, milk yield, management practices, and existing infrastructure. Effective national GHG mitigation plans for the dairy industry should incorporate strategies that account for the diversity within this system. This paper aims to introduce a multi-scale framework to assess the GHG mitigation potential within the Israeli dairy system. It begins by analyzing the GHG intensity per unit of milk produced by a representative sample of 145 farms (20 % of the national dairy farms). It then extrapolates the data to the regional and national scales. The research reveals an average carbon footprint of 1.18 (ranging from 0.8 to 1.64) kg CO 2 e per kilogram of milk (FPCM) over the life cycle up to the farm gate. Upon scaling up, the study estimates the annual carbon footprint of the Israeli dairy industry at 1,777,800 t of CO 2 e. Consequently, this framework highlights areas with significant GHG emissions that require attention and opportunities for national mitigation based on the detailed characteristics of the studied systems., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Landfills are a major source of anthropogenic methane emissions and have been found to produce nitrous oxide, an even more potent greenhouse gas than methane. Intermediate cover soil (ICS) plays a key role in reducing methane emissions but may also result in nitrous oxide production. To assess the potential for microbial methane oxidation and nitrous oxide production, long sequencing reads were generated from ICS microbiome DNA and reads were functionally annotated for 24 samples across ICS at a large landfill in New York. Further, incubation experiments were performed to assess methane consumption and nitrous oxide production with varying amounts of ammonia supplemented. Methane was readily consumed by microbes in the composite ICS and all incubations with methane produced small amounts of nitrous oxide even when ammonia was not supplemented. Incubations without methane produced significantly less nitrous oxide than those incubated with methane. In incubations with methane added, the observed specific rate of methane consumption was 0.776 +/- 0.055 μg CH 4 g dry weight (DW) soil -1  h -1 and the specific rate of nitrous oxide production was 3.64 × 10 -5 +/- 1.30 × 10 -5  μg N 2 O g DW soil -1  h -1 . The methanotrophs Methylobacter and an unclassified genus within the family Methlyococcaceae were present in the original ICS samples and the incubation samples, and their abundance increased during incubations with methane. Genes encoding particulate methane monooxygenase/ ammonia monooxygenase (pMMO) were much more abundant than genes encoding soluble methane monooxygenase (sMMO) across the landfill ICS. Genes encoding proteins that convert hydroxylamine to nitrous oxide were not highly abundant in the ICS or incubation metagenomes. In total, these results suggest that although ammonia oxidation via methanotrophs may result in low levels of nitrous oxide production, ICS microbial communities have the potential to greatly reduce the overall global warming potential of landfill emissions., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Achieving a balance between greenhouse gas mitigation and biomass production in grasslands necessitates optimizing irrigation frequency and nitrogen addition, which significantly influence grassland productivity and soil nitrous oxide emissions, and consequently impact the ecosystem carbon dioxide exchange. This study aimed to elucidate these influences using a controlled mesocosm experiment where bermudagrass (Cynodon dactylon L.) was cultivated under varied irrigation frequencies (daily and every 6 days) with (100 kg ha -1 ) or without nitrogen addition; measurements of net ecosystem carbon dioxide exchange, ecosystem respiration, soil respiration, and nitrous oxide emissions across two cutting events were performed as well. The findings revealed a critical interaction between water-filled pore space, regulated by irrigation, and nitrogen availability, with the latter exerting a more substantial influence on aboveground biomass growth and ecosystem carbon dioxide exchange than water availability. Moreover, the total dry matter was significantly higher with nitrogen addition compared to without nitrogen addition, irrespective of the irrigation frequency. In contrast, soil nitrous oxide emissions were observed to be significantly higher with increased irrigation frequency and nitrogen addition. The effects of nitrogen addition on soil respiration components appeared to depend on water availability, with autotrophic respiration seeing a significant rise with nitrogen addition under limited irrigation (5.4 ± 0.6 μmol m -2 s -1 ). Interestingly, the lower irrigation frequency did not result in water stress, suggesting resilience in bermudagrass. These findings highlight the importance of considering interactions between irrigation and nitrogen addition to optimize water and nitrogen input in grasslands for a synergistic balance between grassland biomass production and greenhouse gas emission mitigation., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Coastal ecosystems such as salt marshes, seagrass meadows, and kelp forests contribute to climate regulation as carbon sinks. However, coastal ecosystems may act as carbon sources as beach wrack accumulations may release greenhouse gases (GHG) during decomposition. The magnitude of GHG emissions of beach wrack accumulations under natural conditions are poorly understood, hampering accurate blue carbon accountings. In this study, we assessed the spatio-temporal variability and environmental factors driving CO 2 , CH 4 and N 2 O emissions from beach wrack accumulations on a temperate sandy beach. Beach wrack accumulations, dominated by Zostera marina and opportunistic brown macroalgae, presented variable spatio-temporal dynamics. Annual beach wrack GHG emissions achieved up to 77,915 mg m -2 d -1 CO 2e (CO 2 equivalents) and varied largely throughout the study period due to interactive effects of temperature, wave exposure, beach wrack biomass moisture, abundance, and species composition. Our findings showed that methane emissions in new, freshly deposited, and in drifting wrack in the water reached up to 100 mg m -2 d -1 , representing up to 57 % of annual CO 2e emissions occurring throughout the year. Nitrous oxide emissions were highly variable and comprised a minor extent (i.e., up to 4 %) of annual CO 2e emissions. Together, wrack CH 4 and N 2 O emissions provided 13.69 g CO 2 m -2 per year to the atmosphere. Our findings indicate that excessive opportunistic macroalgae biomass driven by eutrophication may explain increased CO 2 and N 2 O emissions. We conclude that whilst beach wrack depositions are a natural and essential part of coastal ecosystems, they may provide an extra source of GHG to the atmosphere, potentially counteracting the role of vegetated coastal ecosystems as carbon sinks., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

There has been much concern about microplastic (MP) pollution in marine and soil environments, but attention is gradually shifting towards wetland ecosystems, which are a transitional zone between aquatic and terrestrial ecosystems. This paper comprehensively reviews the sources of MPs in wetland ecosystems, as well as their occurrence characteristics, factors influencing their migration, and their effects on animals, plants, microorganisms, and greenhouse gas (GHG) emissions. It was found that MPs in wetland ecosystems originate mainly from anthropogenic sources (sewage discharge, and agricultural and industrial production) and natural sources (rainfall-runoff, atmospheric deposition, and tidal effects). The most common types and forms of MPs identified in the literature were polyethylene and polypropylene, fibers, and fragments. The migration of MPs in wetlands is influenced by both non-biological factors (the physicochemical properties of MPs, sediment characteristics, and hydrodynamic conditions) and biological factors (the adsorption and growth interception by plant roots, ingestion, and animal excretion). Furthermore, once MPs enter wetland ecosystems, they can impact the resident microorganisms, animals, and plants. They also have a role in global warming because MPs act as unique exogenous carbon sources, and can also influence GHG emissions in wetland ecosystems by affecting the microbial community structure in wetland sediments and abundance of genes associated with GHG emissions. However, further investigation is needed into the influence of MP type, size, and concentration on the GHG emissions in wetlands and the underlying mechanisms. Overall, the accumulation of MPs in wetland ecosystems can have far-reaching consequences for the local ecosystem, human health, and global climate regulation. Understanding the effects of MPs on wetland ecosystems is essential for developing effective management and mitigation strategies to safeguard these valuable and vulnerable environments., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

Limited research has been conducted on ammonia (NH 3 ) volatilization and greenhouse gases (GHGs) emissions in saline-alkali paddy fields, along with complex interaction involving various genes (16sRNA, amoA, narG, nirK, nosZ, and nifH). This study employed mesocosm-scale experiment to investigate NH 3 volatilization and GHGs emissions, focusing on bacterial communities and genic abundance, in saline-alkali paddy fields with desulfurized gypsum (DG) and organic fertilizer (OF) amendments. Compared to the control (CK) treatment, DG and OF treatments reduced methane (CH 4 ) and carbon dioxide (CO 2 ) emissions by 78.05 % and 26.18 %, and 65.84 % and 11.62 %, respectively. However, these treatments increased NH 3 volatilization by 26.26 % and 45.23 %, and nitrous oxide (N 2 O) emission by 41.00 % and 12.31 %. Notably, NH 3 volatilization primarily stemmed from ammonia nitrogen (NH 4 + -N), rather than total nitrogen (TN) in soil and water. N 2 O was mainly produced from nitrate nitrogen (NO 3 - -N) in soil and water, as well as NH 4 + -N in water. The increase in NH 3 volatilization and N 2 O emission in DG and OF treatments, was attributed to the reduced competition among bacterial communities, rather than the increased bacterial activity and genic copies. These findings offer valuable insights for managing nutrient loss and gaseous emissions in saline-alkali paddy fields., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

This study explores the greenhouse gas (GHG) fluxes of nitrous oxide (N 2 O), methane (CH 4 ) and carbon dioxide (CO 2 ) from a two-stage, cold-climate vertical-flow treatment wetland (TW) treating ski area wastewater at 3 °C average water temperature. The system is designed like a modified Ludzack-Ettinger process with the first stage a partially saturated, denitrifying TW followed by an unsaturated nitrifying TW and recycle of nitrified effluent. An intermittent wastewater dosing scheme was established for both stages, with alternating carbon-rich wastewater and nitrate-rich recycle to the first stage. The system has demonstrated effective chemical oxygen demand (COD) and total inorganic nitrogen (TIN) removal in high-strength wastewater over seven years of winter operation. Following two closed-loop, intensive GHG winter sampling campaigns at the TW, the magnitude of N 2 O flux was 2.2 times higher for denitrification than nitrification. CH 4 and N 2 O emissions were strongly correlated with hydraulic loading, whereas CO 2 was correlated with surface temperature. GHG fluxes from each stage were related to both microbial activity and off-gassing of dissolved species during wastewater dosing, thus the time of sampling relative to dosing strongly influenced observed fluxes. These results suggest that estimates of GHG fluxes from TWs may be biased if mass transfer and mechanisms of wastewater application are not considered. Emission factors for N 2 O and CH 4 were 0.27 % as kg-N 2 O-N/kg-TIN removed and 0.04 % kg-CH 4 -C/kg-COD removed, respectively . The system had observed seasonal emissions of 600.5 kg CO 2 equivalent of GHGs estimated over 130-days of operation. These results indicate a need for wastewater treatment processes to mitigate GHGs., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Aerobic methane-oxidizing bacteria (MOB) play a crucial role in mitigating the greenhouse gas methane emission, particularly prevalent in flooded wetlands. The implementation of ridge with no-tillage practices within a rice-rape rotation system proves effective in overcoming the restrictive redox conditions associated with waterlogging. This approach enhances capillary water availability from furrows, especially during periods of low rainfall, thereby supporting plant growth on the ridges. However, the microbe-mediated accumulation of soil organic carbon and nitrogen remains insufficiently understood under this agricultural practice, particularly concerning methane oxidation, which holds ecological and agricultural significance in the rice fields. In this study, the ridge and ditch soils from a 28-year-old ridge with no-tillage rice field experiment were utilized for incubation with 13 C-CH 4 and 15 NN 2 to estimate the methane-oxidizing and N 2 -fixing potentials. Our findings reveal a significantly higher net production of fresh soil organic carbon in the ridge compared to the ditch soil during methane oxidation, with values of 626 and 543 μg 13 C g -1 dry weight soil, respectively. Additionally, the fixed 15 N exhibited a twofold increase in the ridge soil (14.1 μg 15 N g -1 dry weight soil) compared to the ditch soil. Interestingly, the result of DNA-based stable isotope probing indicated no significant differences in active MOB and N 2 fixers between ridge and ditch soils. Both Methylocystis-like type II and Methylosarcina/Methylomonas-like type I MOB catalyzed methane into organic biomass carbon pools. Soil N 2 -fixing activity was associated with the 15 N-labeling of methane oxidizers and non-MOB, such as methanol oxidizers (Hyphomicrobium) and conventional N 2 fixers (Burkholderia). Methane oxidation also fostered microbial interactions, as evidenced by co-occurrence patterns. These results underscore the dual role of microbial methane oxidation - not only as a recognized sink for the potent greenhouse gas methane but also as a source of soil organic carbon and bioavailable nitrogen. This emphasizes the pivotal role of microbial methane metabolism in contributing to soil carbon and nitrogen accumulation in ridge with no-tillage systems., Competing Interests: Declaration of competing interest The authors declare that they have no competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

In the context of global warming, increasingly widespread and frequent freezing and thawing cycles (FTCs) will have profound effects on the biogeochemical cycling of soil carbon and nitrogen. FTCs can increase soil greenhouse gas (GHG) emissions by reducing the stability of soil aggregates, promoting the release of dissolved organic carbon, decreasing the number of microorganisms, inducing cell rupture, and releasing carbon and nitrogen nutrients for use by surviving microorganisms. However, the similarity and disparity of the mechanisms potentially contributing to changes in GHGs have not been systematically evaluated. The present study consolidates the most recent findings on the dynamics of soil carbon and nitrogen, as well as GHGs, in relation to FTCs. Additionally, it analyzes the impact of FTCs on soil GHGs in a systematic manner. In this study, particular emphasis is given to the following: (i) the reaction mechanism involved; (ii) variations in soil composition in different types of land (e.g., forest, peatland, farmland, and grassland); (iii) changes in soil structure in response to cycles of freezing temperatures; (iv) alterations in microbial biomass and community structure that may provide further insight into the fluctuations in GHGs after FTCs. The challenges identified included the extension of laboratory-scale research to ecosystem scales, the performance of in-depth investigation of the coupled effects of carbon, nitrogen, and water in the freeze-thaw process, and analysis of the effects of FTCs through the use of integrated research tools. The results of this study can provide a valuable point of reference for future experimental designs and scientific investigations and can also assist in the analysis of the attributes of GHG emissions from soil and the ecological consequences of the factors that influence these emissions in the context of global permafrost warming., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:YUJUAN WEN reports financial support was provided by National Natural Science Foundation of China. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

The incorporation of straw with decomposing inoculants into soils has been widely recommended to sustain agricultural productivity. However, comprehensive analyses assessing the effects of straw combined with decomposing inoculants on greenhouse gas (GHG) emissions, net primary production (NPP), the net ecosystem carbon budget (NECB), and the carbon footprint (CF) in farmland ecosystems are scant. Here, we carried out a 2-year field study in a wheat cropping system with six treatments: rice straw (S), a straw-decomposing Bacillus subtilis inoculant (K), a straw-decomposing Aspergillus oryzae inoculant (Q), a combination of straw and Bacillus subtilis inoculant (SK), a combination of straw and Aspergillus oryzae inoculant (SQ), and a control with no rice straw or decomposing inoculant (Control). We found that all the treatments resulted in a positive NECB ranging between 838 and 5065 kg C ha -1 . Relative to the Control, the S treatment increased CO 2 emissions by 16%, while considerably enhancing the NECB by 349%. This difference might be attributed to the straw C input and an increase in plant productivity (NPP, 30%). More importantly, in comparison to that in S, the NECB in SK and SQ significantly increased by 27-35% due to the positive response of NPP to the decomposing inoculants. Although the combination of straw and decomposing inoculants yielded a 3% increase in indirect GHG emissions, it also exhibited the lowest CF (0.18 kg CO 2 -eq kg -1 of grain). This result was attributed to the synergistic effects of straw and decomposing inoculants, which reduced direct N 2 O emissions and increased wheat productivity. Overall, the findings of the present study suggested that the combined amendment of straw and decomposing inoculants is an environmentally sustainable management practice in wheat cropping systems that can generate win-win scenarios through improvements in soil C stock, crop productivity, and GHG mitigation., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Increasing population densities and urban sprawl have induced greenhouse gas (GHG) emissions from the soil, and the soil microbiota of urban forests play a critical role in the production and consumption of GHGs, supporting green development. However, the function and potential mechanism of soil bacteria in GHG emissions from forests during urbanization processes need to be better understood. Here, we measured the fluxes of carbon dioxide (CO 2 ), methane (CH 4 ), and nitrous oxide (N 2 O) in Cinnamomum camphora forest soils along an urbanization gradient. 16S amplicon and metagenomic sequencing approaches were employed to examine the structure and potential functions of the soil bacterial community involved in carbon (C) and nitrogen (N) cycling. In this study, the CH 4 and CO 2 emissions from urban forest soils (sites U and G) were significantly greater than those from suburban soils (sites S and M). The N 2 O emissions in the urban center (site U) were 24.0 % (G), 13.8 % (S), and 13.5 % (M) greater than those at the other three sites. These results were related to the increasing bacterial alpha diversity, interactions, and C and N cycling gene abundances (especially those involved in denitrification) in urban forest soils. Additionally, the soil pH and metal contents (K, Ca, Mg) affected key bacterial populations (such as Methylomirabilota, Acidobacteriota, and Proteobacteria) and indicators (napA, nosZ, nrfA, nifH) involved in reducing N 2 O emissions. The soil heavy metal contents (Fe, Cr, Pb) were the main contributors to CH 4 emissions, possibly by affecting methanogens (Desulfobacterota) and methanotrophic bacteria (Proteobacteria, Actinobacteriota, and Patescibacteria). Our study provides new insights into the benefits of conservation-minded urban planning and close-to-nature urban forest management and construction, which are conducive to mitigating GHG emissions and supporting urban sustainable development by mediating the core bacterial population., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Legume-based rotation is commonly recognized for its mitigation efficiency of greenhouse gas (GHG) emissions. However, variations in GHG emission-associated metabolic functions during the legume-vegetable rotation process remain largely uncharacterized. Accordingly, a soybean-radish rotation field experiment was designed to clarify the responses of microbial communities and their GHG emission-associated functional metabolism through metagenomics. The results showed that the contents of soil organic carbon and total phosphorus significantly decreased during the soybean-radish process (P < 0.05), while soil total potassium content and bacterial richness and diversity significantly increased (P < 0.05). Moreover, the predominant bacterial phyla varied, with a decrease in the relative abundance of Proteobacteria and an increase in the relative abundance of Acidobacteria, Gemmatimonadetes, and Chloroflexi. Metagenomics clarified that bacterial carbohydrate metabolism substantially increased during the rotation process, whereas formaldehyde assimilation, methanogenesis, nitrification, and dissimilatory nitrate reduction decreased (P < 0.05). Specifically, the expression of phosphate acetyltransferase (functional methanogenesis gene, pta) and nitrate reductase gamma subunit (functional dissimilatory nitrate reduction gene, narI) was inhibited, indicating of low methane production and nitrogen metabolism. Additionally, the partial least squares path model revealed that the Shannon diversity index was negatively correlated with methane and nitrogen metabolism (P < 0.01), further demonstrating that the response of the soil bacterial microbiome responses are closely linked with GHG-associated metabolism during the soybean-radish rotation process. Collectively, our findings shed light on the responses of soil microbial communities to functional metabolism associated with GHG emissions and provide important insights to mitigate GHG emissions during the rotational cropping of legumes and vegetables., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)

With the rapid development of the economy, household activities have emerged as an important source of greenhouse gas (GHG) emissions, making them a crucial focal point for research in the pursuit of sustainable development and carbon emission reduction. Hulunber, as a typical steppe region in eastern Eurasia, is representative of studying the GHG emissions from household ranches, which are the basic production units in this region. In this paper, based on survey data of 2018 and 2019, we quantified and assessed GHG emissions from household ranches by combining life cycle assessment (LCA) and structural equation modeling (SEM) approaches, with LCA to define household ranches system boundary and SEM to determine the key driving factors of emissions. The results showed that GHG emissions of meat sheep live weight was 23.54 kg CO 2 -eq/kg. The major contributor to household GHG emissions was enteric methane (55.23 %), followed by coal use (20.80 %) and manure management systems (9.16 %), and other contributing factors (14.81 %). The SEM results indicated that the GHG emissions from household ranches were derived primarily by economic level, while the economic level was significantly affected by income. This study also found a significant positive and linear correlation between household GHG emissions and the number of meat sheep (R 2  = 0.89, P < 0.001). The GHG emissions from meat sheep production (67.52 %) were double times greater than household livelihood consumption (32.48 %). These findings emphasized the importance of reducing emissions from meat sheep production and adjusting the energy mix of household livelihood, contributing to the establishment of a low-carbon household livelihood operation., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Herbicide residue and greenhouse gas (GHG) emission are two main problems in the paddy rice field, which have barely been considered simultaneously. Herein, a bensulfuron-methyl (BSM)-degrading bacterium named Acinetobacter YH0317 was successfully immobilized on two kinds of biochars and subsequently applied in the paddy soil. The BSM removal rate of Acinetobacter YH0317 immobilized boron-doping biochar (BBC) was 80.42% after 30 d, which was significantly higher than that of BBC (39.05%) and Acinetobacter YH0317 (49.10%) applied alone. BBC acting as an immobilized carrier could enable Acinetobacter YH0317 to work in harsh and complex environment and thus improve the BSM removal efficiency. The addition of Acinetobacter YH0317 immobilized BBC (TP5) significantly improved the soil physicochemical properties (pH, SOC, and NH 4 + -N) and increased the diversity of soil microbial community compared to control group (CG). Meanwhile, Acinetobacter YH0317 immobilized BBC reduced the CO 2 -equivalent emission by 41.0%. Metagenomic sequencing results revealed that the decreasing CO 2 emission in TP5 was correlated with carbon fixation gene (fhs), indicating that fhs gene may play an important role in reducing CO 2 emission. The work presents a practical and supportive technique for the simultaneous achievement on the soil purification and GHG emission reduction in paddy soil., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

With the implementation of municipal solid waste source segregation, the enormous sorted biogenic waste has become an issue that needs to be seriously considered. Anaerobic digestion, which can produce biogas and extract floating oil for biodiesel production, is the most prevalent treatment in China for waste management and greenhouse gas (GHG) emissions reduction, in accordance with Sustainable Development Goal 13 of the United Nations. Herein, a large-scale biogas plant with a capacity of 1000 tonnes of biogenic waste (400 tonnes of restaurant biogenic waste and 600 tonnes of kitchen biogenic waste) per day was investigated onsite using material flow analysis, and the parts of the biogas plant were thoroughly analyzed, especially the pretreatment system for biogenic waste impurity removal and homogenization. The results indicated that the loss of the total biodegradable organic matter was 41.8% (w/w) of daily feedstock and the loss of biogas potential was 18.8% (v/v) of daily feedstock. Life cycle assessment revealed that the 100-year GHG emissions were -61.2 kgCO 2 -eq per tonne biogenic waste. According to the sensitivity analysis, pretreatment efficiency, including biodegradable organic matter recovery and floating oil extraction, considerably affected carbon reduction potential. However, when the pretreatment efficiency deteriorated, GHG benefits of waste source segregation and the subsequent biogenic waste anaerobic digestion would be reduced., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

As global greenhouse gas emissions increase and fossil energy sources decline dramatically, the energy transition is at the heart of many countries' development initiatives. As a biomass resource, straw plays a positive role in energy transformation and environmental improvement. However, there is still a challenge to explore the best options and models for straw production and utilization of green and efficient biomass energy in agricultural systems. This study establishes an economic-environmental-resource synergistic Straw Green recycling optimization model based on straw-electricity-biochar-biogas core (Straw Green recycling optimization model, SGROM). Firstly, we explore the effects of biochar return to the field on crop yield and greenhouse gas emission by Meta-analysis method, and on this basis, we construct SGROM to weigh the three objectives of economic-greenhouse gas emission-resource utilization, and explore the best allocation ratio between four utilization methods of straw: power generation, biochar preparation, biogas and derivatives preparation and sale, so as to obtain a straw recycling and efficient low-carbon utilization model. Exploring the response of straw green utilization patterns to crop market prices with the help of deep learning methods, SGROM has been applied to the main grain producing areas in the Sanjiang Plain of China, and the results of comparison with the traditional straw utilization (TSU) model show that the greenhouse gas emissions per unit of production value of SGROM are 19.66 % lower than that of TSU model, the electricity consumption is saved by 2.00 %, and the optimal ratios of straw for power generation, biogas and biochar production, and sale are 1.00 %, 10.75 %, 62.11 % and 26.14 %. The economic benefits and total greenhouse gas emissions of the integrated straw utilization mode are better than those of the single straw utilization mode, proving the superiority of SGROM in optimizing the straw utilization mode., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

The one-time application of common urea blended with controlled-release urea (CRU) is considered effective for improving nitrogen use efficiency and grain yield and reducing the greenhouse gas emissions of summer maize in intensive agricultural systems. However, the trade-off between the economic and environmental performances of different blended fertilizer treatments for different maize varieties remains unclear. Therefore, a consecutive two-year field experiment was conducted in the North China Plain to study the effects of different ratios of CRU and common urea on the yield, nitrous oxide (N 2 O) emissions, yield-scaled total N 2 O emissions, greenhouse gas intensity (GHGI), and net ecosystem economic benefit (NEEB) in 2021 and 2022. Four N fertilizer treatments with equal rate at 180 kg N ha -1 were applied as N180U (all Urea), N180C1(1/3CRU), N180C2(2/3CRU), and N180C (all CRU), and two maize varieties (JNK728-yellow ripe variety and ZD958-green ripe variety) were used. The N180C1 and N180C2 treatments produced the highest grain yield in varieties JNK728 and ZD958 (9.4-11.5 t ha -1 and 9.0-11.0 t ha -1 ), respectively. Compared to the N180U treatment (conventional method), the N180C1 treatment reduced the GHGI (24.8 %-25.9 %) and increased the NEEB (33.1 %-33.4 %) in the JNK728 variety, whereas the N180C2 treatment reduced the GHGI (16.9 %-28.8 %) and increased the NEEB (27.2 %-48.1 %) in the ZD958 variety. The study concludes that a one-time application of blended nitrogen fertilizer in suitable varieties can minimize the GHGI and maximize the NEEB, which is an effective strategy for balancing yield and nitrogen efficiency in the summer maize system in the North China Plain., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Cover crop cultivation can be a vital strategy for mitigating climate change in agriculture, by increasing soil carbon stocks and resource efficiency within the cropping system. Another mitigation option is to harvest the cover crop and use the biomass to replace greenhouse gas-intensive products, such as fossil fuels. Harvesting cover crop biomass could also reduce the risk of elevated N 2 O emissions associated with cover crop cultivation under certain conditions, which would offset much of the mitigation potential. However, harvesting cover crops also reduces soil carbon sequestration potential, as biomass is removed from the field, and cultivation of cover crops requires additional field operations that generate greenhouse gas emissions. To explore these synergies and trade-offs, this study investigated the life cycle climate effect of cultivating an oilseed radish cover crop under different management strategies in southern Scandinavia. Three alternative scenarios (Incorporation of biomass into soil; Mowing and harvesting aboveground biomass; Uprooting and harvesting above- and belowground biomass) were compared with a reference scenario with no cover crop. Harvested biomass in the Mowing and Uprooting scenarios was assumed to be transported to a biogas plant for conversion to upgraded biogas, with the digestate returned to the field as an organic fertiliser for the subsequent crop. The climate change mitigation potential of cover crop cultivation was found to be 0.056, 0.58 and 0.93 Mg CO 2 -eq ha -1 in the Incorporation, Mowing and Uprooting scenario, respectively. The Incorporation scenario resulted in the highest soil carbon sequestration, but also the greatest soil N 2 O emissions. Substitution of fossil diesel showed considerable mitigation potential, especially in the Uprooting scenario, where biogas production was highest. Sensitivity analysis revealed a strong impact of time of cover crop establishment, with earlier establishment leading to greater biomass production and thus greater mitigation potential., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Global agricultural systems face one of the greatest sustainability challenges: meeting the growing demand for food without leaving a negative environmental footprint. United States (US) and China are the two largest economies and account for 39 % of total global greenhouse gases (GHG) emissions into the atmosphere. No-till is a promising land management option that allows agriculture to better adapt and mitigate climate change effects compared to traditional tillage. However, the efficacy of no-till for mitigating GHG is still debatable. In this meta-analysis, we comprehensively assess the impact of no-till (relative to traditional tillage) on GHG mitigation potential and crop productivity in different agroecological systems and management regimes in the US and China. Overall, no-till in China did not change crop yields, although soil CO 2 (8 %) and N 2 O (12 %) emissions decreased significantly, while soil CH 4 emissions increased by 12 %. In contrast to Chinese no-till, a significant improvement in crop yields (up to 12 %) was recorded on US cropland under no-till. Moreover, significant decreases in soil N 2 O (21 %) and CH 4 (12 %) emissions were observed. Of the three cropping systems, only wheat showed significant reduction in CO 2 , N 2 O and CH 4 emissions in the Chinese no-till system. In the case of US, no-till soybean-rice and maize cropping systems demonstrated significant emission reductions for N 2 O and CO 2 , respectively. Interestingly, yields of no-till maize in China and rice in US exceeded those of other no-till cereals. In China, no-till on medium-texture soils resulted in significant reductions in GHG emissions and higher crop yields compared to other soil types. In both countries, the relatively higher crop yields under irrigated versus non-irrigated no-till and the significant yield differences on fine textured soils under US no-till are likely due to the substantial N 2 O reductions. In summary, crop yield disparities from no-till between China and the US were related to the insignificant effects on controlling CH 4 emissions and successfully mitigating N 2 O, respectively. This study comprehensively demonstrates how cropping system and pedoclimatic conditions influence the relative effectiveness of no-till in both countries., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Climate change due to increased greenhouse gas emissions (GHG) in the atmosphere has been consistently observed since the mid-20th century. The profound influence of global climate change on greenhouse gas (GHG) emissions, encompassing carbon dioxide (CO 2 ), methane (CH 4 ), and nitrous oxide (N 2 O), has established a vital feedback loop that contributes to further climate change. This intricate relationship necessitates a comprehensive understanding of the underlying feedback mechanisms. By examining the interactions between global climate change, soil, and GHG emissions, we can elucidate the complexities of CO 2 , CH 4 , and N 2 O dynamics and their implications. In this study, we evaluate the global climate change relationship with GHG globally in 246 countries. We find a robust positive association between climate and GHG emissions. By 2100, GHG emissions will increase in all G7 countries and China while decreasing in the United Kingdom based on current economic growth policies, resulting in a net global increase, suggesting that climate-driven increase in GHG and climate variations impact crop production loss due to soil impacts and not provide climate adaptation. The study highlights the diverse strategies employed by G7 countries in reducing GHG emissions, with France leveraging nuclear power, Germany focusing on renewables, and Italy targeting its industrial and transportation sectors. The UK and Japan are making significant progress in emission reduction through renewable energy, while the US and Canada face challenges due to their industrial activities and reliance on fossil fuels., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Inc. All rights reserved.)

Mangrove forests, crucial carbon-rich ecosystems, are increasingly vulnerable to soil carbon loss and greenhouse gas (GHG) emissions due to human disturbance. However, the contribution of mangrove trees to GHG emissions remains poorly understood. This study monitored CO 2 , CH 4 , and N 2 O fluxes from the stems of two mangrove species, native Kandelia obovata (KO) and exotic Sonneratia apetala (SA), at three heights (0.7 m, 1.2 m, and 1.7 m) during the dry winter period on Qi'ao Island, Pearl River Estuary, China. Heartwood samples were analyzed to identify potential functional groups related to gas fluxes. Our study found that tree stems acted as both sinks and sources for N 2 O (ranging from -9.49 to 28.35 μg m -2  h -1 for KO and from -6.73 to 28.95 μg m -2  h -1 for SA) and CH 4 . SA exhibited significantly higher stem CH 4 flux (from -26.67 to 97.33 μg m -2  h -1 ) compared to KO (from -44.13 to 88.0 μg m -2  h -1 ) (P < 0.05). When upscaled to the community level, both species were net emitters of CH 4 , contributing approximately 4.68 % (KO) and 0.51 % (SA) to total CH 4 emissions. The decrease in stem CH 4 flux with increasing height, indicates a soil source. Microbial analysis in the heartwood using the KEGG database indicated aceticlastic methanogenesis as the dominant CH 4 pathway. The presence of methanogens, methanotrophs, denitrifiers, and nitrifiers suggests microbial involvement in CH 4 and N 2 O production and consumption. Remarkably, the dominance of Cyanobacteria in the heartwood microbiome (with the relative abundance of 97.5 ± 0.6 % for KO and 99.1 ± 0.2 % for SA) implies roles in carbon and nitrogen fixation for mangroves coping with nitrogen limitation in coastal wetlands, and possibly in CH 4 production. Although the present study has limitations in sampling duration and area, it highlights the significant role of tree stems in GHG emissions which is crucial for a holistic evaluation of the global carbon sequestration capability of mangrove ecosystems. Future research should broaden spatial and temporal scales to enhance the accuracy of upscaling tree stem gas fluxes to the mangrove ecosystem level., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Biochar application in rice production reduces nitrogen loss and greenhouse gases. We conducted in situ experiments for 3 years, with N 210 B 0 (210 kg N ha -1 ) as the control. Two biochar application methods (B 1 :15 t ha -1 biochar applied once and B 2 : biochar applied three times at 5 t ha -1  yr -1 ) combined with two nitrogen levels (N 210 : 210 kg N ha -1 and N 168 : 168 kg N ha -1 ) were used. Soil physicochemical properties, CH 4 and N 2 O emissions, functional gene abundance, rice yield, and nitrogen use efficiency were analyzed. Both methods improved the physicochemical properties of the soil, however, B 1 was less effective than B 2 in increasing soil pH, bulk density, organic carbon, total nitrogen, and microbial biomass nitrogen in year 3. B 1 had a higher CH 4 emission mitigation effect than B 2 in 3 consecutive years, mainly due to the higher pmoA gene abundance. B 1 showed a higher reduction effect of N 2 O emissions compared to B 2 in year 1, but the opposite was observed in years 2 and 3. B 2 had a higher abundance of AOB, nirK, and nosZ genes compared to B 1 in year 3. Compared with N 210 B 0 , rice yields were increased by 9.1 %, 9.6 %, and 3.6 % with N 210 B 1 , N 210 B 2, and N 168 B 2 , respectively, over 3 years, while N 168 B 1 improved yields in the previous 2 years. Biochar improved nitrogen use efficiency over 3 consecutive years directly due to increased use efficiency of panicle fertilizer; the effect of B 1 was greater than that of B 2 during years 1 and 2, while the opposite was observed in year 3. Both Biochar applied once and three times appeared to be promising practices to increase yield and mitigate GHGs. From the GHGI perspective, the biochar applied once combined with 168 kg N ha -1 can further improve nitrogen use efficiency, and reduce GHGs without hindering improvements in rice yield., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)