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2. List of Contributors

Author

Agrawal, Pankaj B., primary, Akhtar, Yasmin, additional, Alallah, Jubara, additional, Alhassen, Ziad, additional, Altit, Gabriel, additional, AlZubaidi, Abbas, additional, Amlani, Lahin M., additional, Antelo, Martin, additional, Athalye-Jape, Gayatri, additional, Badarch, Jargalsaikhan, additional, Baer, Gerri, additional, Bagga, Nitasha, additional, Bahr, Timothy M., additional, Bar-Cohen, Yaniv, additional, M. Barr, Stephanie, additional, Bauer, Andrew J., additional, Bearer, Cynthia F., additional, Beckman, Ross M., additional, Beltempo, Marc, additional, Bembea, Melania M., additional, Berlin, Sheila, additional, Berry, Shandeigh N., additional, Bhandari, Vineet, additional, Bhatia, Shaifali, additional, Bhombal, Shazia, additional, Bigelow, Elaine O., additional, Blevins, Carley, additional, Boppana, Suresh, additional, Boss, Renee, additional, Brooks, Sandra, additional, Buonocore, Giuseppe, additional, Burnsed, Jennifer, additional, Calabria, Andrew C., additional, Carrasco, Melisa, additional, Carter, Brian S., additional, Chandrasekharan, Praveen, additional, Chavez-Valdez, Raul, additional, Choleva, Lauryn, additional, Christensen, Robert D., additional, Chung, Wendy K., additional, Coggins, Sarah A., additional, Cooke, David W., additional, Cummings, Laura, additional, Currie, Erin R., additional, Davidson, Joanne O., additional, Davis, Jonathan M., additional, Day-Richardson, Colby L., additional, De Leon, Diva D., additional, Dedhia, Kavita, additional, Dendi, Alvaro, additional, Deshpande, Anita, additional, Dionne, Janis M., additional, Donda, Keyur, additional, Donohue, Lee, additional, Doyle, Jefferson J., additional, Dulkerian, Susan J., additional, Dumpa, Vikramaditya, additional, Duncan, Andrea F., additional, Dunham, Alexandra M., additional, Ecret, DiAnn, additional, Ellis, Kelstan, additional, El-Metwally, Dina, additional, Etchill, Eric W., additional, Ethawi, Yahya, additional, Everett, Allen D., additional, Fabres, Jorge, additional, Felling, Ryan J., additional, Fenton, Tanis R., additional, Flannery, Dustin D., additional, Flynn, Joseph T., additional, Fredenburg, Michaelene, additional, Fuqua, John, additional, Garcia, Alejandro V., additional, Garzon, Steven, additional, Gauda, Estelle B., additional, Thompson, Marisa Gilstrop, additional, Goldenberg, Barton, additional, Salazar, Andres J. Gonzalez, additional, Goodwin, Julie E., additional, Goudy, Steven L., additional, Graham, Ernest, additional, Grauerholz, Kathryn, additional, Groves, Mari L., additional, Guillot, Mireille, additional, Gunn, Alistair J., additional, Gupta, Arjun, additional, Hackam, David J., additional, Hidalgo, Joaquin, additional, Honcharuk, Erin, additional, Htun, Zeyar, additional, Hudak, Mark L., additional, Driscoll, Colleen A. Hughes, additional, Huisman, Thierry A.G.M., additional, Jabroun, Mireille, additional, Jackson, Eric M., additional, Jain, Naveen, additional, Jain, Rajesh, additional, Jelin, Angie, additional, Jelin, Eric, additional, Juul, Sandra E., additional, Kaufman, David A., additional, BMBS, Alison Kent,, additional, Khuder, Sundos, additional, Kovler, Mark L., additional, Kraus, Courtney L., additional, Krishnamurthy, Ganga, additional, Kukora, Stephanie K., additional, Kumar, Ashok, additional, Kunisaki, Shaun M., additional, Kuper-Sassé, Margaret, additional, Kwiatkowski, David M., additional, Lakshminrusimha, Satyan, additional, Laventhal, Naomi T., additional, Lawrence, Shelley M., additional, Lee-Winn, Angela E., additional, Leuthner, Steven, additional, Lewallen, Laura, additional, Lewis, Tamorah R., additional, Liken, Hillary B., additional, Liubšys, Arūnas, additional, Lui, Kei, additional, Maheshwari, Akhil, additional, Maitre, Nathalie L., additional, Makker, Kartikeya, additional, Mammen, Cherry, additional, J. Martin, Richard, additional, Mattos Castellano, María, additional, Maxwell, Jessie R., additional, McFarlane, Renske, additional, McLemore, Gabrielle, additional, McNelis, Kera M., additional, McPherson, Christopher, additional, Mietzsch, Ulrike, additional, Milante, Rachel R., additional, Miller, Jena L., additional, Mukhopadhyay, Sagori, additional, Mynak, Mimi L., additional, Nasr, Isam W., additional, Natarajan, Niranjana, additional, Navaneethan, Hema, additional, Nees, Shannon N., additional, Nguyen, Mai, additional, Noori, Shahab, additional, Odackal, Namrita J., additional, Ohls, Robin K., additional, Ostrander, Betsy E., additional, Pammi, Mohan, additional, Parimi, Prabhu S., additional, Park, Albert, additional, Patil, Monika S., additional, Pereira, Elaine M., additional, Premkumar, Muralidhar H., additional, Price-Douglas, Webra, additional, Puopolo, Karen M., additional, Rabe, Heike, additional, M. Rahman, Mohammad, additional, Reber, Kristina, additional, Kallem, Venkat Reddy, additional, Repka, Michael X., additional, Rhee, Daniel S., additional, Ringle, Megan L., additional, Rohrer, Allison, additional, Romero, Christopher J., additional, Ryan, Marisa A., additional, Sampah, Maame E.S., additional, Sanchez-Valle, Amarilis, additional, Sant’Anna, Guilherme M., additional, Saugstad, Ola D., additional, RobertH., Anne and, additional, Schacht, John P., additional, Schelonka, Robert L., additional, Schofield, Erin E., additional, Selewski, David T., additional, Shah, Prakesh S., additional, Shih, Jessica G., additional, Sibinga, Erica M.S., additional, Sigal, Winnie, additional, Sims, Brian, additional, Singh, Rachana, additional, Singh, Srijan, additional, Snyder, Donna, additional, Sobrero, Helena, additional, Sponseller, Paul D., additional, Stafstrom, Carl E., additional, Steflik, Heidi J., additional, Sun, Lisa R., additional, Sundararajan, Sripriya, additional, Taylor, Sarah N., additional, Terrin, Norma, additional, Thacker, Prolima G., additional, Thébaud, Bernard, additional, Toms, Rune, additional, Torres, Benjamin A., additional, Tunkel, David E., additional, Umandap, Christine H., additional, Chaves, Diana Vargas, additional, Vento, Maximo, additional, Walsh, Jonathan, additional, Walter, Jolan, additional, Weaver, Meaghann S., additional, Weimer, Kristin, additional, Weller, Jennine, additional, Winter, Lindy W., additional, Wu, Tai-Wei, additional, and Yau, Mabel, additional

Published
2024
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3. High performance of coating hydroxyapatite layer on 316L stainless steel using ultrasonically and alkaline pretreatment

Author

Ahmad Fadli, Agung Prabowo, Silvia Reni Yenti, Feblil Huda, Ayla Annisa Liswani, and Donda Lamsinar Br Hutauruk

Subjects
Pretreatment, Dip Coating, Hydroxyapatite, Coating Strength, Stainless Steel, Science (General), Q1-390
Abstract

One of the metals used for bone implants is 316L Stainless Steel, which is succesfully coated with hydroxyapatite to increase its low biocompatibility. Therefore, this study aims to carry out sonication, alkali, and heating treatment on 316L Stainless Steel substrates, determine the effect of temperature (A) sonication time (B), acetone concentration (C), bidirectional interaction of sonication temperature and time (AB), bidirectional interaction of sonication time and acetone concentration (BC), bidirectional interaction of sonication temperature and acetone concentration (AC) and the three-way interaction of sonication temperature, time and acetone concentration (ABC), a suitable empirical model for the coating process, and concentration of cleaning solution on the bond strength of the hydroxyapatite layer. The empirical model of the bond strength of the hydroxyapatite layer used was y = 426.1 – 11.50A – 19.25B – 6.229C + 0.6505AB + 0.1944AC + 0.2737 BCE – 0.00933 ABC with an R2 value of 99.49%. The result showed that the layer's bond strength increases with the sonication temperature. It also showed that the longer the sonication time and the acetone concentration, the lower the bond strength value. The highest hydroxyapatite bond strength was produced at a sonication temperature, time, acetone concentration volume and bond strength of 45 °C, 15 min, 99%, and 91.35 Mpa, respectively.

Published
2023
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4. Cenozoic history of Antarctic glaciation and climate from onshore and offshore studies

Author

M. McKay, Robert, primary, Escutia, Carlota, additional, De Santis, Laura, additional, Donda, Federica, additional, Duncan, Bella, additional, Gohl, Karsten, additional, Gulick, Sean, additional, Hernández-Molina, Javier, additional, Hillenbrand, Claus-Dieter, additional, Hochmuth, Katharina, additional, Kim, Sookwan, additional, Kuhn, Gerhard, additional, Larter, Robert, additional, Leitchenkov, German, additional, H. Levy, Richard, additional, R. Naish, Tim, additional, O’Brien, Phil, additional, F. Pérez, Lara, additional, E. Shevenell, Amelia, additional, and Williams, Trevor, additional

Published
2022
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5. miR-155 Overexpression in OT-1 CD8+ T Cells Improves Anti-Tumor Activity against Low-Affinity Tumor Antigen

Author

Gwennaëlle C. Monnot, Amaia Martinez-Usatorre, Evripidis Lanitis, Silvia Ferreira Lopes, Wan-Chen Cheng, Ping-Chih Ho, Melita Irving, George Coukos, Alena Donda, and Pedro Romero

Subjects
Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
Abstract

Therapy by adoptive transfer of ex vivo-expanded tumor-infiltrating or genetically modified T cells may lead to impressive clinical responses. However, there is a need to improve in vivo persistence and functionality of the transferred T cells, in particular, to face the highly immunosuppressive environment of solid tumors. Here, we investigate the potential of miR-155, a microRNA known to play an important role in CD8+ T cell fitness. We show that forced expression of miR-155 in tumor antigen-specific T cells improves the tumor control of B16 tumors expressing a low-affinity antigen ligand. Importantly, miR-155-transduced T cells exhibit increased proliferation and effector functions associated with a higher glycolytic activity independent of exogenous glucose. Altogether, these data suggest that miR-155 may optimize the antitumor activity of adoptively transferred low-affinity tumor-infiltrating lymphocytes (TILs), in particular, by rendering them more resistant to the glucose-deprived environment of solid tumors. Thus, transgenic expression of miR-155 may enable therapeutic targeting of self-antigen-specific T cells in addition to neoantigen-specific ones. Keywords: microRNA-155, CD8+ T cells, TCR antigen affinity, therapeutic vaccine, adoptive cell transfer, oncoimmunology

Published
2020
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6. List of contributors

Author

Bentley, Michael J., primary, Bijl, Peter, additional, Bostock-Lyman, Helen, additional, Bowen, Melissa, additional, Brinkuis, Henk, additional, Carter, Lionel, additional, Chorley, Hannah K., additional, Colleoni, Florence, additional, De Santis, Laura, additional, DeConto, Robert M., additional, Dickinson, Warren, additional, Dolan, Aisling M., additional, Donda, Federica, additional, Duncan, Bella, additional, Escutia, Carlota, additional, Flierdt, Tina van de, additional, Florindo, Fabio, additional, Francis, Jane, additional, Galeotti, Simone, additional, Gasson, Edward G.W., additional, Ghezzo, Claudio, additional, Gohl, Karsten, additional, Golledge, Nicholas R., additional, Gore, Damian B., additional, Grant, Georgia R., additional, Gulick, Sean, additional, H. Levy, Richard, additional, Halberstadt, Anna Ruth W., additional, Harwood, David M., additional, Hein, Andrew S., additional, Hernández-Molina, Javier, additional, Hillenbrand, Claus-Dieter, additional, Hochmuth, Katharina, additional, Hutchinson, David, additional, Jamieson, Stewart, additional, Kennedy-Asser, Alan, additional, Kim, Sookwan, additional, Kleinschmidt, Georg, additional, Kowalewski, Douglas E., additional, Kuhn, Gerhard, additional, Lanci, Luca, additional, Larter, Robert, additional, Leitchenkov, German, additional, Levy, Richard H., additional, Lewis, Adam R., additional, McKay, Robert M., additional, Meloni, Antonio, additional, Meyers, Stephen R., additional, R. Naish, Tim, additional, Ohneiser, Christian, additional, O’Brien, Phil, additional, Patterson, Molly O., additional, Pérez, Lara F., additional, Powell, Ross, additional, Sangiorgi, Francesca, additional, Santis, Laura De, additional, Sauermilch, Isabel, additional, Shevenell, Amelia E., additional, Siegert, Martin, additional, Sluijs, Appy, additional, Stocchi, Paolo, additional, Talarico, Franco, additional, Uenzelmann-Neben, Gabriele, additional, van de Flierdt, Tina, additional, Verret, Marjolaine, additional, White, Duanne A., additional, Williams, Trevor, additional, Wilson, David J., additional, and Wilson, Gary, additional

Published
2021
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8. First assessment of an area potentially suitable for underground hydrogen storage in Italy

Author

Mattera, S., Donda, F., Tinivella, U., Barison, E., Le Gallo, Y., Vincent, C., Mattera, S., Donda, F., Tinivella, U., Barison, E., Le Gallo, Y., and Vincent, C.

Abstract

Hydrogen storage can help achieve climate change and reduce greenhouse gas emissions. This paper presents a first assessment of the suitability of northeastern Italy for underground hydrogen storage (UHS). The study focuses on the analysis of publicly available well data, which allowed identifying geological formations potentially suitable for UHS. The most promising area, known as the “Treviso Area” consists of both saline aquifers and depleted gas fields. One of the key petrophysical properties, i.e. porosity, was calculated for each of the five wells revealing conditions potentially suitable for UHS by applying empirical formulas to geophysical log data. For the two depleted gas fields, a hydrogen injection simulation was also performed. This work is a pioneer study and lays the foundation for hopeful further analyses, which could help implement the recently launched “North Adriatic Hydrogen Valley” initiative.

Published
2023

9. Mammalian Target of Rapamycin Complex 2 Controls CD8 T Cell Memory Differentiation in a Foxo1-Dependent Manner

Author

Lianjun Zhang, Benjamin O. Tschumi, Isabel C. Lopez-Mejia, Susanne G. Oberle, Marten Meyer, Guerric Samson, Markus A. Rüegg, Michael N. Hall, Lluis Fajas, Dietmar Zehn, Jean-Pierre Mach, Alena Donda, and Pedro Romero

Subjects
Biology (General), QH301-705.5
Abstract

Summary: Upon infection, antigen-specific naive CD8 T cells are activated and differentiate into short-lived effector cells (SLECs) and memory precursor cells (MPECs). The underlying signaling pathways remain largely unresolved. We show that Rictor, the core component of mammalian target of rapamycin complex 2 (mTORC2), regulates SLEC and MPEC commitment. Rictor deficiency favors memory formation and increases IL-2 secretion capacity without dampening effector functions. Moreover, mTORC2-deficient memory T cells mount more potent recall responses. Enhanced memory formation in the absence of mTORC2 was associated with Eomes and Tcf-1 upregulation, repression of T-bet, enhanced mitochondrial spare respiratory capacity, and fatty acid oxidation. This transcriptional and metabolic reprogramming is mainly driven by nuclear stabilization of Foxo1. Silencing of Foxo1 reversed the increased MPEC differentiation and IL-2 production and led to an impaired recall response of Rictor KO memory T cells. Therefore, mTORC2 is a critical regulator of CD8 T cell differentiation and may be an important target for immunotherapy interventions. : Zhang et al. demonstrate that mTORC2 deficiency favors CD8 T cell memory differentiation during the primary antigen-specific T cell response to Listeria infection. The effects result from higher Foxo1 transcriptional activity without dampening effector functions. They also show enhanced recall responses by mTORC2-deficient memory CD8 T cells. Keywords: Rictor, mTORC2, Foxo1, CD8 T cell, infection

Published
2016
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10. Chapter3: Cenozoic history of Antarctic glaciation and climate from onshore and offshore studies

Author

Florindo, Fabio, Siegert, Martin J., De Santis, Laura, Naish, Tim, McKay, Robert, Escutia, Carlota, Donda, F., Duncan, B., Gohl, Karsten, Gulick, Sean P. S., Hernandez-Molina, F. J., Hillenbrand, Claus-Dieter, Hochmuth, Katharina, Kim, S., Kuhn, Gerhard, Larter, Robert D., Leitchenkov, German, Levy, Richard H., O'Brien, Philip, Perez, Lara, Shevenell, Amelia E., Williams, Trevor, Florindo, Fabio, Siegert, Martin J., De Santis, Laura, Naish, Tim, McKay, Robert, Escutia, Carlota, Donda, F., Duncan, B., Gohl, Karsten, Gulick, Sean P. S., Hernandez-Molina, F. J., Hillenbrand, Claus-Dieter, Hochmuth, Katharina, Kim, S., Kuhn, Gerhard, Larter, Robert D., Leitchenkov, German, Levy, Richard H., O'Brien, Philip, Perez, Lara, Shevenell, Amelia E., and Williams, Trevor

Published
2022

11. Cenozoic history of Antarctic glaciation and climate from onshore and offshore studies

Author

Richard H. Levy, Claus-Dieter Hillenbrand, Laura De Santis, Katharina Hochmuth, Robert D Larter, Amelia E. Shevenell, F. J. Hernández-Molina, Lara F. Pérez, Philip E O'Brien, Carlota Escutia, S. Kim, German Leitchenkov, Federica Donda, Karsten Gohl, Gerhard Kuhn, Bella Duncan, Robert M. McKay, Tim R Naish, Trevor Williams, and Sean P. S. Gulick

Subjects
Ice-sheet dynamics, geography, geography.geographical_feature_category, 010504 meteorology & atmospheric sciences, Continental shelf, Ocean current, Antarctic ice sheet, 010502 geochemistry & geophysics, 01 natural sciences, Oceanography, 13. Climate action, Paleoclimatology, 14. Life underwater, Glacial period, Offshore drilling, Cenozoic, Geology, 0105 earth and related environmental sciences
Abstract

The past three decades have seen a sustained and coordinated effort to refine the seismic stratigraphic framework of the Antarctic margin that has underpinned the development of numerous geological drilling expeditions from the continental shelf and beyond. Integration of these offshore drilling datasets covering the Cenozoic era with Antarctic inland datasets, provides important constraints that allow us to understand the role of Antarctic tectonics, the Southern Ocean biosphere, and Cenozoic ice sheet dynamics and ice sheet–ocean interactions on global climate as a whole. These constraints are critical for improving the accuracy and precision of future projections of Antarctic ice sheet behaviour and changes in Southern Ocean circulation. Many of the recent advances in this field can be attributed to the community-driven approach of the Scientific Committee on Antarctic Research (SCAR) Past Antarctic Ice Sheet Dynamics (PAIS) research programme and its two key subcommittees: Paleoclimate Records from the Antarctic Margin and Southern Ocean (PRAMSO) and Palaeotopographic-Palaeobathymetric Reconstructions. Since 2012, these two PAIS subcommittees provided the forum to initiate, promote, coordinate and study scientific research drilling around the Antarctic margin and the Southern Ocean. Here we review the seismic stratigraphic margin architecture, climatic and glacial history of the Antarctic continent following the break-up of Gondwanaland in the Cretaceous, with a focus on records obtained since the implementation of PRAMSO. We also provide a forward-looking approach for future drilling proposals in frontier locations critically relevant for assessing future Antarctic ice sheet, climatic and oceanic change.

Published
2022
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12. List of contributors

Author

Michael J. Bentley, Peter Bijl, Helen Bostock-Lyman, Melissa Bowen, Henk Brinkuis, Lionel Carter, Hannah K. Chorley, Florence Colleoni, Laura De Santis, Robert M. DeConto, Warren Dickinson, Aisling M. Dolan, Federica Donda, Bella Duncan, Carlota Escutia, Tina van de Flierdt, Fabio Florindo, Jane Francis, Simone Galeotti, Edward G.W. Gasson, Claudio Ghezzo, Karsten Gohl, Nicholas R. Golledge, Damian B. Gore, Georgia R. Grant, Sean Gulick, Richard H. Levy, Anna Ruth W. Halberstadt, David M. Harwood, Andrew S. Hein, Javier Hernández-Molina, Claus-Dieter Hillenbrand, Katharina Hochmuth, David Hutchinson, Stewart Jamieson, Alan Kennedy-Asser, Sookwan Kim, Georg Kleinschmidt, Douglas E. Kowalewski, Gerhard Kuhn, Luca Lanci, Robert Larter, German Leitchenkov, Adam R. Lewis, Robert M. McKay, Antonio Meloni, Stephen R. Meyers, Tim R. Naish, Christian Ohneiser, Phil O’Brien, Molly O. Patterson, Lara F. Pérez, Ross Powell, Francesca Sangiorgi, Isabel Sauermilch, Amelia E. Shevenell, Martin Siegert, Appy Sluijs, Paolo Stocchi, Franco Talarico, Gabriele Uenzelmann-Neben, Marjolaine Verret, Duanne A. White, Trevor Williams, David J. Wilson, and Gary Wilson

Published
2021
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13. Cenozoic history of Antarctic glaciation and climate from onshore and offshore studies

Author

Florindo, Fabio, Siegert, Martin, De Santis, Laura, Naish, Tim, McKay, Robert M., Escutia, Carlota, Donda, Federica, Duncan, Bella, Gohl, Karsten, Gulick, Sean, Hernández-Molina, Javier, Hillenbrand, Claus-Dieter, Hochmuth, Katharina, Kim, Sookwan, Kuhn, Gerhard, Larter, Robert, Leitchenkov, German, Levy, Richard H., Naish, Tim R., O’Brien, Phil, Perez, Lara F., Shevenell, Amelia E., Williams, Trevor, Florindo, Fabio, Siegert, Martin, De Santis, Laura, Naish, Tim, McKay, Robert M., Escutia, Carlota, Donda, Federica, Duncan, Bella, Gohl, Karsten, Gulick, Sean, Hernández-Molina, Javier, Hillenbrand, Claus-Dieter, Hochmuth, Katharina, Kim, Sookwan, Kuhn, Gerhard, Larter, Robert, Leitchenkov, German, Levy, Richard H., Naish, Tim R., O’Brien, Phil, Perez, Lara F., Shevenell, Amelia E., and Williams, Trevor

Abstract

The past three decades have seen a sustained and coordinated effort to refine the seismic stratigraphic framework of the Antarctic margin that has underpinned the development of numerous geological drilling expeditions from the continental shelf and beyond. Integration of these offshore drilling datasets covering the Cenozoic era with Antarctic inland datasets, provides important constraints that allow us to understand the role of Antarctic tectonics, the Southern Ocean biosphere, and Cenozoic ice sheet dynamics and ice sheet–ocean interactions on global climate as a whole. These constraints are critical for improving the accuracy and precision of future projections of Antarctic ice sheet behaviour and changes in Southern Ocean circulation. Many of the recent advances in this field can be attributed to the community-driven approach of the Scientific Committee on Antarctic Research (SCAR) Past Antarctic Ice Sheet Dynamics (PAIS) research programme and its two key subcommittees: Paleoclimate Records from the Antarctic Margin and Southern Ocean (PRAMSO) and Palaeotopographic-Palaeobathymetric Reconstructions. Since 2012, these two PAIS subcommittees provided the forum to initiate, promote, coordinate and study scientific research drilling around the Antarctic margin and the Southern Ocean. Here we review the seismic stratigraphic margin architecture, climatic and glacial history of the Antarctic continent following the break-up of Gondwanaland in the Cretaceous, with a focus on records obtained since the implementation of PRAMSO. We also provide a forward-looking approach for future drilling proposals in frontier locations critically relevant for assessing future Antarctic ice sheet, climatic and oceanic change.

Published
2021

14. Recombinant fusion proteins for targeting dendritic cell subsets in therapeutic cancer vaccine

Author

Stéphanie Corgnac, Alena Donda, Natalia K. Botelho, and Pedro Romero

Subjects
Antigen, Cancer research, Priming (immunology), Cytotoxic T cell, Cancer vaccine, Dendritic cell, Biology, Cell activation, Fusion protein, Tumor antigen
Abstract

Dendritic cells (DCs) are professional antigen-presenting cells, which are optimal for the priming of a T cell response against pathogens and tumors. Therefore, many efforts are made to develop therapeutic cancer vaccines which preferentially target the antigen to DC subsets. To this aim, we developed two types of recombinant fusion proteins, which favor antigen delivery to pro-inflammatory DCs as well as the crosstalk between specialized subpopulations of DCs. The first approach combines peptide/CpG vaccination with the recruitment of iNKT cells to the tumor site via CD1d-antitumor scFv fusion proteins. The second approach is targeting the tumor antigen to cross-presenting Xcr1+ DCs via a fusion protein made of Xcl1 fused to a synthetic long peptide followed by an IgG1 Fc fragment. Both strategies allow a potent tumor-specific CD8 T cell response associated with tumor regression or tumor growth delay depending on the model. In the case of iNKT cell activation, the strategy relies on a strong IL-12 release by splenic DCs, while in the second case, the T cell response is strictly dependent on the presence of Xcr1+ cross-presenting DCs.

Published
2020
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15. Continental slope and rise geomorphology seaward of the Totten Glacier, East Antarctica (112E-122E)

Author

O'Brien, P E, Post, A. L., Edwards, S, Martin, T, CABURLOTTO, ANDREA, DONDA, FEDERICA, LEITCHENKOV, GERMAN, ROMEO, ROBERTO, Duffy, M, Evangelinos, D, Opdyke, Bradley, Armand, Leanne, O'Brien, P E, Post, A. L., Edwards, S, Martin, T, CABURLOTTO, ANDREA, DONDA, FEDERICA, LEITCHENKOV, GERMAN, ROMEO, ROBERTO, Duffy, M, Evangelinos, D, Opdyke, Bradley, and Armand, Leanne

Abstract

The continental slope and rise seaward of the Totten Glacier and the Sabrina Coast, East Antarctica features continental margin depositional systems with high sediment input and consistent along-slope current activity. Understanding their genesis is a necessary step in interpreting the paleoenvironmental records they contain. Geomorphic mapping using a systematic multibeam survey shows variations in the roles of downslope and along slope sediment transport influenced by broad-scale topography and oceanography. The study area contains two areas with distinct geomorphology. Canyons in the eastern part of the area have concave thalwegs, are linked to the shelf edge and upper slope and show signs of erosion and deposition along their beds suggesting cycles of activity controlled by climate cycles. Ridges between these canyons are asymmetric with crests close to the west bank of adjacent canyons and are mostly formed by westward advection of fine sediment lofted from turbidity currents and deposition of hemipelagic sediment. They can be thought of as giant levee deposits. The ridges in the western part of the area have more gently sloping eastern flanks and rise to shallower depths than those in the east. The major canyon in the western part of the area is unusual in having a convex thalweg; it is likely fed predominantly by mass movement from the flanks of the adjacent ridges with less sediment input from the shelf edge. The western ridges formed by accretion of suspended sediment moving along the margin as a broad plume in response to local oceanography supplemented with detritus originating from the Totten Glacier. This contrasts with interpretations of similar ridges described from other parts of Antarctica which emphasise sediment input from canyons immediately up-current. The overall geomorphology of the Sabrina Coast slope is part of a continuum of mixed contourite-turbidite systems identified on glaciated margins.

Published
2020

17. Contributors

Author

Acosta, Juan, primary, Allee, Rebecca J., additional, Althaus, Franziska, additional, Alvarez, German, additional, Amblas, David, additional, Anderson, Tara J., additional, Archambault, Philippe, additional, Armstrong, Roy A., additional, Bäck, Saara, additional, Baker, Elaine K., additional, Baptist, Martin, additional, Barrett, Neville, additional, Barrie, J. Vaughn, additional, Bartolomé, Rafael, additional, Bashmachnikov, Igor, additional, Bates, Richard, additional, Battershill, Chris, additional, Bax, Nicholas J., additional, Beaman, Robin J., additional, Beaudoin, Yannick C., additional, Bell, Trevor, additional, Bøe, Reidulv, additional, Borja, Ángel, additional, Bowden, David A., additional, Henriques, Andreia Braga, additional, Bridge, Thomas, additional, Brooke, Brendan P., additional, Buhl-Mortensen, Lene, additional, Buhl-Mortensen, Pål, additional, Busquets, Pere, additional, Calafat, Antonio, additional, Campos, Aldino S., additional, Canals, Miquel, additional, Catarino, Diana, additional, Ceri James, J.W., additional, Chiocci, Francesco L., additional, Clark, Malcolm R., additional, Cochran, Susan A., additional, Cochrane, Guy R., additional, Coggan, Roger A., additional, Coiras, Enrique, additional, Colaço, Ana, additional, Collier, Jenny S., additional, Collin, Antoine, additional, Conway, Kim W., additional, Copeland, Alison, additional, Cremer, Jenny, additional, D’Angelo, Silvana, additional, Dankers, Norbert, additional, Darbyshire, Teresa, additional, David, Andrew W., additional, Degraer, Steven, additional, De Mol, Ben, additional, De Mol, Lies, additional, De Santis, Laura, additional, Devillers, Rodolphe, additional, Díaz-del-Río, Víctor, additional, Diesing, Markus, additional, Dijkman, Elze, additional, Dolan, Margaret F.J., additional, Donda, Federica, additional, Done, Terry, additional, Doornenbal, Pieter J., additional, Dorokhov, Dmitry, additional, Dove, Dayton, additional, Du Four, Isabelle, additional, Duran, Ruth, additional, Durán-Muñoz, Pablo, additional, Edinger, Evan, additional, Elvenes, Sigrid, additional, Etherington, Lisa, additional, Ezhova, Elena, additional, Falace, Annalisa, additional, Fenner, Douglas, additional, Fernández-Salas, Luis M., additional, Fiorentino, Andrea, additional, Flemming, Robert, additional, Furey, Thomas, additional, Galparsoro, Ibon, additional, Gary Greene, H., additional, Germán Rodríguez, J., additional, Getsiv-Clemons, Julia E.R., additional, Giacomello, Eva, additional, Gibbs, Ann E., additional, Gili, Josep Maria, additional, Gonçalves, João, additional, Gordini, Emiliano, additional, Gori, Andrea, additional, Gràcia, Eulàlia, additional, Guinan, Janine, additional, Hagan, Annelise B., additional, Hamylton, Sarah, additional, Harney, Jodi, additional, Harris, Peter T., additional, Heap, Andrew D., additional, Heifetz, Jonathan, additional, Henriet, Jean-Pierre, additional, Heyman, William D., additional, Hilário, Ana, additional, Hill, Nicole, additional, Hirsch, Emily R., additional, Hodnesdal, Hanne, additional, Hogrefe, Kyle R., additional, Humber, Stuart R., additional, Huvenne, Veerle A.I., additional, Isidro, Eduardo J., additional, Johnstone, Glenn, additional, Dañobeitia, Juan Jose, additional, Kaleb, Sara, additional, Kaskela, Anu M., additional, Kloser, Rudy J., additional, Kobara, Shinichi, additional, Kocheshkova, Olga, additional, Koppers, Anthony A.P., additional, Kostylev, Vladimir E., additional, Kotilainen, Aarno T., additional, Lamarche, Geoffroy, additional, Lavoie, Caroline, additional, Leahy, Yvonne, additional, LeBlanc, Philippe, additional, Legorburu, Irati, additional, Leinikki, Jouni, additional, Lindenbaum, Charles, additional, Linklater, Michelle, additional, Lo Iacono, Claudio, additional, Long, Bernard, additional, López-González, Nieves, additional, Lucieer, Vanessa, additional, McArthur, Matthew A., additional, Mackay, Kevin, additional, Mackie, Andrew S.Y., additional, Marocco, Ruggero, additional, Martins, Ana, additional, Martorelli, Eleonora, additional, Masson, Douglas G., additional, MacKenzie, Monique, additional, Mendonça, Ana, additional, Menezes, Gui, additional, Fernández-Salas, L. Miguel, additional, Mitchell, Neil C., additional, Mleczko, Richard, additional, Moerkerke, Geert, additional, Morando, Angela, additional, Morato, Telmo, additional, Mountjoy, Joshu, additional, Muñoz, Araceli, additional, Murillo, F. Javier, additional, Muxika, Iñigo, additional, Naar, David F., additional, Nichol, Scott L., additional, Nodder, Scott D., additional, Norcross, Brenda L., additional, O’Brien, Philip E., additional, Orejas, Covadonga, additional, Pallentin, Arne, additional, Pascual, Marta, additional, Pattenden, Abigail D.C., additional, Pearce, Bryony, additional, Picard, Kim, additional, Pinho, Mário R., additional, Pinnion, Jennifer, additional, Pizarro, Oscar, additional, Porteiro, Filipe M., additional, Post, Alexandra L., additional, Puig, Pere, additional, Puotinen, Marji, additional, Rabaut, Marijn, additional, Rees, E.Ivor S., additional, Requena, Susana, additional, Reynolds, Jennifer R., additional, Ribó, Marta, additional, Riddle, Martin J., additional, Rintoul, Stephen R., additional, Rivera, Jesus, additional, Roberts, Jed T., additional, Robinson, Karen A., additional, Rooney, Sean C., additional, Rowden, Ashley A., additional, Rueda, José L., additional, Ryabchuk, Daria, additional, Sagar, Stephen, additional, Sanderson, William G., additional, Santos, Ricardo S., additional, Sayago-Gil, Miriam, additional, Seiler, Jan, additional, Serrano, Alberto, additional, Shotwell, S.Kalei, additional, Smith, Jodie, additional, Smith, John R., additional, Smith, Samantha, additional, Smith, Stephen J., additional, Spencer, Tom, additional, Singh, Hanumant, additional, Sivkov, Vadim, additional, Stark, Jonathan S., additional, Stewart, Ian J., additional, Stieglitz, Thomas C., additional, Tappin, David R., additional, Tempera, Fernando, additional, Thorsnes, Terje, additional, Todd, Brian J., additional, Trusel, Luke, additional, Tunis, Giorgio, additional, Tyler, Paul A., additional, Valentine, Page C., additional, van Dalfsen, Jan A., additional, van Dijk, Thaiënne A.G.P., additional, van Duin, Willem, additional, van Heteren, Sytze, additional, Van Lancker, Vera, additional, van Landeghem, Katrien J.J., additional, van Overmeeren, Ronnie A., additional, Van Rooij, David, additional, Vázquez, Juan T., additional, Verbruggen, Koen, additional, Verdier, Anne-Laure, additional, Verfaillie, Els, additional, Wakefield, W. Waldo, additional, Webster, Jody M., additional, Clift, Leslie Whaylen, additional, Whitmire, Curt E., additional, Williams, Alan, additional, Williams, Stefan, additional, Woodroffe, Colin D., additional, Wright, Dawn J., additional, Wroblewski, Joseph, additional, Wysoczanski, Richard J., additional, Yamanaka, K.Lynne, additional, Yoklavich, Mary, additional, and Zhamoida, Vladimir, additional

Published
2012
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20. Train lateral analysis of an offshore gas compressor train experiencing high unbalance sensitivity

Author

J.M. Byrne, C.H. Cloud, J.A. Vázquez, O. Donda, and M. He

Subjects
Vibration, Engineering, business.industry, Centrifugal compressor, Gas lift, Submarine pipeline, Structural engineering, business, Sensitivity (explosives), Gas compressor, Marine engineering
Abstract

This case history summarizes the investigation of vibration problems experienced by two centrifugal compressors in offshore gas lift/export service. Since their commissioning, both barreltype compressors have consistently required field balancing due to fouling, and even immediately after overhaul and shop balancing.

Published
2012
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21. Contributors

Author

Juan Acosta, Rebecca J. Allee, Franziska Althaus, German Alvarez, David Amblas, Tara J. Anderson, Philippe Archambault, Roy A. Armstrong, Saara Bäck, Elaine K. Baker, Martin Baptist, Neville Barrett, J. Vaughn Barrie, Rafael Bartolomé, Igor Bashmachnikov, Richard Bates, Chris Battershill, Nicholas J. Bax, Robin J. Beaman, Yannick C. Beaudoin, Trevor Bell, Reidulv Bøe, Ángel Borja, David A. Bowden, Andreia Braga Henriques, Thomas Bridge, Brendan P. Brooke, Lene Buhl-Mortensen, Pål Buhl-Mortensen, Pere Busquets, Antonio Calafat, Aldino S. Campos, Miquel Canals, Diana Catarino, J.W. Ceri James, Francesco L. Chiocci, Malcolm R. Clark, Susan A. Cochran, Guy R. Cochrane, Roger A. Coggan, Enrique Coiras, Ana Colaço, Jenny S. Collier, Antoine Collin, Kim W. Conway, Alison Copeland, Jenny Cremer, Silvana D’Angelo, Norbert Dankers, Teresa Darbyshire, Andrew W. David, Steven Degraer, Ben De Mol, Lies De Mol, Laura De Santis, Rodolphe Devillers, Víctor Díaz-del-Río, Markus Diesing, Elze Dijkman, Margaret F.J. Dolan, Federica Donda, Terry Done, Pieter J. Doornenbal, Dmitry Dorokhov, Dayton Dove, Isabelle Du Four, Ruth Duran, Pablo Durán-Muñoz, Evan Edinger, Sigrid Elvenes, Lisa Etherington, Elena Ezhova, Annalisa Falace, Douglas Fenner, Luis M. Fernández-Salas, Andrea Fiorentino, Robert Flemming, Thomas Furey, Ibon Galparsoro, H. Gary Greene, J. Germán Rodríguez, Julia E.R. Getsiv-Clemons, Eva Giacomello, Ann E. Gibbs, Josep Maria Gili, João Gonçalves, Emiliano Gordini, Andrea Gori, Eulàlia Gràcia, Janine Guinan, Annelise B. Hagan, Sarah Hamylton, Jodi Harney, Peter T. Harris, Andrew D. Heap, Jonathan Heifetz, Jean-Pierre Henriet, William D. Heyman, Ana Hilário, Nicole Hill, Emily R. Hirsch, Hanne Hodnesdal, Kyle R. Hogrefe, Stuart R. Humber, Veerle A.I. Huvenne, Eduardo J. Isidro, Glenn Johnstone, Juan Jose Dañobeitia, Sara Kaleb, Anu M. Kaskela, Rudy J. Kloser, Shinichi Kobara, Olga Kocheshkova, Anthony A.P. Koppers, Vladimir E. Kostylev, Aarno T. Kotilainen, Geoffroy Lamarche, Caroline Lavoie, Yvonne Leahy, Philippe LeBlanc, Irati Legorburu, Jouni Leinikki, Charles Lindenbaum, Michelle Linklater, Claudio Lo Iacono, Bernard Long, Nieves López-González, Vanessa Lucieer, Matthew A. McArthur, Kevin Mackay, Andrew S.Y. Mackie, Ruggero Marocco, Ana Martins, Eleonora Martorelli, Douglas G. Masson, Monique MacKenzie, Ana Mendonça, Gui Menezes, L. Miguel Fernández-Salas, Neil C. Mitchell, Richard Mleczko, Geert Moerkerke, Angela Morando, Telmo Morato, Joshu Mountjoy, Araceli Muñoz, F. Javier Murillo, Iñigo Muxika, David F. Naar, Scott L. Nichol, Scott D. Nodder, Brenda L. Norcross, Philip E. O’Brien, Covadonga Orejas, Arne Pallentin, Marta Pascual, Abigail D.C. Pattenden, Bryony Pearce, Kim Picard, Mário R. Pinho, Jennifer Pinnion, Oscar Pizarro, Filipe M. Porteiro, Alexandra L. Post, Pere Puig, Marji Puotinen, Marijn Rabaut, E.Ivor S. Rees, Susana Requena, Jennifer R. Reynolds, Marta Ribó, Martin J. Riddle, Stephen R. Rintoul, Jesus Rivera, Jed T. Roberts, Karen A. Robinson, Sean C. Rooney, Ashley A. Rowden, José L. Rueda, Daria Ryabchuk, Stephen Sagar, William G. Sanderson, Ricardo S. Santos, Miriam Sayago-Gil, Jan Seiler, Alberto Serrano, S.Kalei Shotwell, Jodie Smith, John R. Smith, Samantha Smith, Stephen J. Smith, Tom Spencer, Hanumant Singh, Vadim Sivkov, Jonathan S. Stark, Ian J. Stewart, Thomas C. Stieglitz, David R. Tappin, Fernando Tempera, Terje Thorsnes, Brian J. Todd, Luke Trusel, Giorgio Tunis, Paul A. Tyler, Page C. Valentine, Jan A. van Dalfsen, Thaiënne A.G.P. van Dijk, Willem van Duin, Sytze van Heteren, Vera Van Lancker, Katrien J.J. van Landeghem, Ronnie A. van Overmeeren, David Van Rooij, Juan T. Vázquez, Koen Verbruggen, Anne-Laure Verdier, Els Verfaillie, W. Waldo Wakefield, Jody M. Webster, Leslie Whaylen Clift, Curt E. Whitmire, Alan Williams, Stefan Williams, Colin D. Woodroffe, Dawn J. Wright, Joseph Wroblewski, Richard J. Wysoczanski, K.Lynne Yamanaka, Mary Yoklavich, and Vladimir Zhamoida

Published
2012
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22. Cenozoic ice sheet history from east Antarctic Wilkes Land continental margin sediments

Author

L. De Santis, Carlota Escutia, Alan K Cooper, Giuliano Brancolini, Robert B. Dunbar, S.L. Eittreim, and Federica Donda

Subjects
Global and Planetary Change, geography, geography.geographical_feature_category, Cenozoic, Ice stream, Wilkes Land, Antarctic ice sheet, Antarctic sea ice, Oceanography, Ice shelf, Ice-sheet model, Paleontology, Ice core, East Antarctic Ice Sheet, Wisconsin glaciation, Glacial evolution, Ice sheet, Geomorphology, Geology
Abstract

10 figures, 3 tables., The long-term history of glaciation along the East Antarctic Wilkes Land margin, from the time of the first arrival of the ice sheet to the margin, through the significant periods of Cenozoic climate change is inferred using an integrated geophysical and geological approach. We postulate that the first arrival of the ice sheet to the Wilkes Land margin resulted in the development of a large unconformity (WL-U3) between 33.42 and 30 Ma during the early Oligocene cooling climate trend. Above WL-U3, substantial margin progradation takes place with early glacial strata (e.g., outwash deposits) deposited as low-angle prograding foresets by temperate glaciers. The change in geometry of the prograding wedge across unconformity WL-U8 is interpreted to represent the transition, at the end of the middle Miocene “climatic optimum” (14–10 Ma), from a subpolar regime with dynamic ice sheets (i.e., ice sheets come and go) to a regime with persistent but oscillatory ice sheets. The steep foresets above WL-U8 likely consist of ice proximal sediments (i.e., water-lain till and debris flows) deposited when grounded ice-sheets extended into the shelf. On the continental rise, shelf progradation above WL-U3 results in an up-section increase in the energy of the depositional environment (i.e., seismic facies indicative of more proximal turbidite and of bottom contour current deposition from the deposition of the lower WL-S5 sequence to WL-S7). Maximum rates of sediment delivery to the rise occur during the development of sequences WL-S6 and WL-S7, which we infer to be of middle Miocene age. During deposition of the two uppermost sequences, WL-S8 and WL-S9, there is a marked decrease in the sediment supply to the lower continental rise and a shift in the depocenters to more proximal areas of the margin. We believe WL-S8 records sedimentation during the final transition from a dynamic to a persistent but oscillatory ice sheet in this margin (14–10 Ma). Sequence WL-S9 forms under a polar regime during the Pliocene–Pleistocene, when most sediment delivered to the margin is trapped in the outer shelf and slope-forming steep prograding wedges. During the warmer but still polar, Holocene, biogenic sediment accumulates quickly in deep inner-shelf basins during the high-stand intervals. These sediments contain an ultrahigh resolution (annual to millennial) record of climate variability. Validation of our inferences about the nature and timing of Wilkes Land glacial sequences can be achieved by deep sampling (i.e., using IODP-type techniques). The most complete record of the long-term history of glaciation in this margin can be obtained by sampling both (1) the shelf, which contains the direct (presence or no presence of ice) but low-resolution record of glaciation, and (2) the rise, which contains the distal (cold vs. warm) but more complete record of glaciation. The Wilkes Land margin is the only known Antarctic margin where the presumed “onset” of glaciation unconformity (WL-U3) can be traced from shelf to the abyssal plain, allowing links between the proximal and the distal records of glaciation to be established. Additionally, the eastern segment of the Wilkes Land margin may be more sensitive to climate change because the East Antarctic Ice Sheet (EAIS) is grounded below sea level. Therefore, the Wilkes Land margin is not only an ideal location to obtain the long-term EAIS history but also to obtain the shorter-term record of ice sheet fluctuations at times that the East Antarctic Ice Sheet is thought to have been more stable (after 15 Ma-recent)., This paper has been possible through a coordinated approach to understanding glacial history in the Wilkes Land margin. The approach has depended upon the considerable efforts of coproponents and contributors of site survey data for two IODP drilling proposals, Proposal #482 (Escutia et al.) and an IODP Ancillary Project Letter (Dunbar et al.). Besides the coauthors, coproponents and contributors include, in alphabetical order, Xavier Crosta, Eugene Domack, Takemi Ishihara, Amy Leventer, Rick Murray, Phil O'Brien, and Manabu Tanahashi. This manuscript was greatly improved thanks to the constructive reviews provided by Eugene Domack, German Leitchenkov, and Phil O'Brien.

Published
2005

23. The effect of using concave surfaces as grinding media

Author

F.L. von Krüger, Antônio Eduardo Clark Peres, M.A.R. Drummond, and J.D. Donda

Subjects
Engineering drawing, Materials science, Grind, Specific surface area, Fineness, Ball (bearing), Charge density, SPHERES, Crushing action, Composite material, Grinding
Abstract

This paper presents an analysis of the effect of utilizing crushing bodies of special shapes as grinding medium. The crushing bodies utilized in this study were modified spheres with a concave portion. Grinding efficiency is expected to increase with the increasing available surface area of the grinding medium. A grinding ball with concavity has a smaller volume than a sphere of the same radius. Therefore, the convex-concave ball has a smaller mass with the same surface area, providing a larger specific surface area and a greater charge density. In the case of contact between the convex and concave surfaces, the probability of a crushing action over larger particles is higher than for smaller particles, causing preferential grinding of larger particles. Experiments were carried out in laboratory and consisted of batches of the same feed, ground first with conventional grinding balls. Part of the grinding medium of conventional grinding balls was then replaced by special concave-convex balls, increasing the specific surface area of the grinding balls. The results of the grinding experiments showed an effective selectivity of the grind, and in some cases, an increase in the fineness of the ground product, for a percentage of the concave area in the charge up to about 10%. Increasing the percentage of the concave bodies, the relative motion of the charge units became more irregular, causing a negative impact on grinding efficiency.

Published
2000
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24. Cenozoic ice sheet history from east Antarctic Wilkes Land continental margin sediments.

Author

Escutia, Carlota, De Santis, Laura, Donda, F., Dunbar, R.B., Cooper, A.K., Brancolini, G., Eittreim, S.L., Escutia, Carlota, De Santis, Laura, Donda, F., Dunbar, R.B., Cooper, A.K., Brancolini, G., and Eittreim, S.L.

Abstract

The long-term history of glaciation along the East Antarctic Wilkes Land margin, from the time of the first arrival of the ice sheet to the margin, through the significant periods of Cenozoic climate change is inferred using an integrated geophysical and geological approach. We postulate that the first arrival of the ice sheet to the Wilkes Land margin resulted in the development of a large unconformity (WL-U3) between 33.42 and 30 Ma during the early Oligocene cooling climate trend. Above WL-U3, substantial margin progradation takes place with early glacial strata (e.g., outwash deposits) deposited as low-angle prograding foresets by temperate glaciers. The change in geometry of the prograding wedge across unconformity WL-U8 is interpreted to represent the transition, at the end of the middle Miocene “climatic optimum” (14–10 Ma), from a subpolar regime with dynamic ice sheets (i.e., ice sheets come and go) to a regime with persistent but oscillatory ice sheets. The steep foresets above WL-U8 likely consist of ice proximal sediments (i.e., water-lain till and debris flows) deposited when grounded ice-sheets extended into the shelf. On the continental rise, shelf progradation above WL-U3 results in an up-section increase in the energy of the depositional environment (i.e., seismic facies indicative of more proximal turbidite and of bottom contour current deposition from the deposition of the lower WL-S5 sequence to WL-S7). Maximum rates of sediment delivery to the rise occur during the development of sequences WL-S6 and WL-S7, which we infer to be of middle Miocene age. During deposition of the two uppermost sequences, WL-S8 and WL-S9, there is a marked decrease in the sediment supply to the lower continental rise and a shift in the depocenters to more proximal areas of the margin. We believe WL-S8 records sedimentation during the final transition from a dynamic to a persistent but oscillatory ice sheet in this margin (14–10 Ma). Sequence WL-S9 forms under a polar r

Published
2005

25. Estrogen receptor regulates MyoD gene expression by preventing AP-1-mediated repression.

Author

Pedraza-Alva G, Zingg JM, Donda A, and Pérez-Martínez L

Subjects
Animals, Estrogens metabolism, Estrogens pharmacology, JNK Mitogen-Activated Protein Kinases metabolism, Mice, Myoblasts, Skeletal drug effects, Myoblasts, Skeletal metabolism, Proto-Oncogene Proteins c-fos metabolism, Transcription, Genetic drug effects, Gene Expression Regulation, Muscle Development genetics, MyoD Protein genetics, Receptors, Estrogen metabolism, Transcription Factor AP-1 metabolism
Abstract

Cell growth and differentiation are opposite events in the myogenic lineage. Growth factors block the muscle differentiation program by inducing the expression of transcription factors that negatively regulate the expression of muscle regulatory genes like MyoD. In contrast, extracellular clues that induce cell cycle arrest promote MyoD expression and muscle differentiation. Thus, the regulation of MyoD expression is critical for muscle differentiation. Here we show that estrogen induces MyoD expression in mouse skeletal muscle in vivo and in dividing myoblasts in vitro by relieving the MyoD promoter from AP-1 negative regulation through a mechanism involving estrogen receptor/AP-1 protein-protein interactions but independent of the estrogen receptor DNA binding activity.

Published
2009
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26. TTF-2 does not appear to be a key mediator of the effect of cyclic AMP on thyroglobulin gene transcription in primary cultured dog thyrocytes.

Author

Pouillon V, Pichon B, Donda A, and Christophe D

Subjects
Animals, Cells, Cultured, Colforsin pharmacology, DNA-Binding Proteins genetics, DNA-Binding Proteins metabolism, Dogs, Electrophoresis, Polyacrylamide Gel, Forkhead Transcription Factors, Genes, Reporter genetics, Nuclear Proteins analysis, Nuclear Proteins metabolism, Peroxidase genetics, Promoter Regions, Genetic genetics, Protein Binding, Repressor Proteins genetics, Repressor Proteins metabolism, Sulfur Radioisotopes, Transcription, Genetic genetics, Transfection genetics, Cyclic AMP metabolism, DNA-Binding Proteins analysis, Gene Expression Regulation genetics, Repressor Proteins analysis, Thyroglobulin genetics, Thyroid Gland metabolism
Abstract

TTF-2 is a thyroid-specific winged-helix transcription factor which has been proposed to play a key role in the hormonal control of thyroglobulin and thyroperoxidase genes transcription in FRTL-5 cells. We have analyzed TTF-2 DNA-binding activity in primary cultures of dog thyrocytes maintained in control condition or in the presence of the cAMP agonist forskolin. Binding of 35S-labelled nuclear proteins to the TTF-2 recognition sequence identified the presence of two molecular species of 41.5 and 42.5 kDa. TTF-2 DNA-binding activity was clearly detectable in nuclear extracts from unstimulated cells and appeared increased in forskolin-treated cells. Thus, the presence of TTF-2 DNA-binding activity does not correlate with the cAMP-dependent activity of thyroglobulin and thyroperoxidase genes in this cell system. In addition, the mutation of the TTF-2 binding site in the thyroglobulin promoter resulted in a very reduced but still clearly cAMP-dependent promoter activity when assayed by transient expression in the same cells. These results do not support a dominant role for TTF-2 in the cAMP-dependent control of thyroglobulin gene transcription in primary cultured thyrocytes.

Published
1998
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