1. Preferential uptake of antibody targeted calcium phosphosilicate nanoparticles by metastatic triple negative breast cancer cells in co-cultures of human metastatic breast cancer cells plus bone osteoblasts.
- Author
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Bussard KM, Gigliotti CM, Adair BM, Snyder JM, Gigliotti NT, Loc WS, Wilczynski ZR, Liu ZK, Meisel K, Zemanek C, Mastro AM, Shupp AB, McGovern C, Matters GL, and Adair JH
- Subjects
- 3T3 Cells, Animals, Coculture Techniques, Female, Humans, Mice, Triple Negative Breast Neoplasms pathology, Antibodies metabolism, Calcium Compounds metabolism, Nanoparticles, Neoplasm Metastasis, Osteoblasts cytology, Silicates metabolism, Triple Negative Breast Neoplasms metabolism
- Abstract
Calcium phosphosilicate nanoparticles (CPSNPs) are bioresorbable nanoparticles that can be bioconjugated with targeting molecules and encapsulate active agents and deliver them to tumor cells without causing damage to adjacent healthy tissue. Data obtained in this study demonstrated that an anti-CD71 antibody on CPSNPs targets these nanoparticles and enhances their internalization by triple negative breast cancer cells in-vitro. Caspase 3,7 activation, DNA damage, and fluorescent microscopy confirmed the apoptotic breast cancer response caused by targeted anti-CD71-CPSNPs encapsulated with gemcitabine monophosphate, the active metabolite of the chemotherapeutic gemcitabine used to treat cancers including breast and ovarian. Targeted anti-CD71-CPSNPs encapsulated with the fluorophore, Rhodamine WT, were preferentially internalized by breast cancer cells in co-cultures with osteoblasts. While osteoblasts partially internalized anti-CD71-GemMP-CPSNPs, their cell growth was not affected. These results suggest that CPSNPs may be used as imaging tools and selective drug delivery systems for breast cancer that has metastasized to bone., (Copyright © 2021 Elsevier Inc. All rights reserved.)
- Published
- 2021
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