1. Arthrospira platensis transglutaminase derived antioxidant peptide-packed electrospun chitosan/ poly (vinyl alcohol) nanofibrous mat accelerates wound healing, in vitro, via inducing mouse embryonic fibroblast proliferation.
- Author
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Sannasimuthu A, Ramani M, Paray BA, Pasupuleti M, Al-Sadoon MK, Alagumuthu TS, Al-Mfarij AR, Arshad A, Mala K, and Arockiaraj J
- Subjects
- Animals, Antioxidants chemistry, Antioxidants metabolism, Cell Proliferation drug effects, Cells, Cultured, Chitosan chemistry, Humans, Mice, NIH 3T3 Cells, Nanofibers chemistry, Particle Size, Peptides chemistry, Peptides metabolism, Polyvinyl Alcohol chemistry, Surface Properties, Transglutaminases metabolism, Antioxidants pharmacology, Fibroblasts drug effects, Peptides pharmacology, Spirulina enzymology, Transglutaminases chemistry, Wound Healing drug effects
- Abstract
In this present study, we have carried out the antioxidant function of transglutaminase (TG) identified from Arthrospira platensis (Ap) transcriptome. The antioxidant peptide ML11 (MLRSIGIPARL) has been predicted from the transglutaminase core domain and the peptide's free radical scavenging potential was evaluated and it shows that it functions on a dose dependent manner. The ML11 peptide cell toxicity was analysed in the human blood leucocytes which resulted no cytotoxic activity in any of the cell population. Moreover, the nanofibre mat encapsulated with antioxidant peptide ML11 was prepared by electrospinning technique. The antioxidant peptide ML11 encapsulated mat showed increase in fibre diameter compared to the chitosan polyvinyl alcohol blended mat. The change in the crystalline behaviour of both chitosan and polyvinyl alcohol polymer to the amorphous nature was determined by X-ray diffraction at the broad band between 20 and 30° (2θ°). FTIR revealed the functional groups which present in the polymer as well as the interaction between their components of chitosan (CS) and polyvinyl alcohol (PVA). The fibre retains the antioxidant activity due to the peptide encapsulated by scavenging the intracellular ROS that was confirmed by flowcytometry and fluorescence microscopy. The ML11 peptide encapsulated mat showed no cytotoxicity in the NIH-3T3 mouse embryonic fibroblast cells. Also, ML11 peptide encapsulated fibre showed potential wound healing activity in NIH-3T3 cells. Taken altogether, the study indicates that the wound healing potential of the ML11 peptide encapsulated nano fibre mat may be used as biopharmaceutical drug., (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Published
- 2020
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