37 results on '"Almeida, C. Marisa R."'
Search Results
2. Occurrence of microplastics and metals in European seabass produced in different aquaculture systems: Implications for human exposure, risk, and food safety.
- Author
-
Matias RS, Gomes S, Barboza LGA, Almeida CMR, Marques A, Guilhermino L, and Valente LMP
- Subjects
- Animals, Humans, Food Safety, Environmental Monitoring, Food Contamination analysis, Risk Assessment, Seafood analysis, Environmental Exposure statistics & numerical data, Bass metabolism, Aquaculture, Water Pollutants, Chemical analysis, Microplastics analysis, Metals analysis
- Abstract
Microplastics (MPs) are emerging contaminants of increasing concern as they may cause adverse effects and carry other contaminants, which may potentially compromise human health. Despite occurring in aquatic ecosystems worldwide, the knowledge about MP presence in different aquaculture systems and their potential impact on seafood products is still limited. This study aimed to determine the levels of MPs in water, feed, and European seabass (Dicentrarchus labrax) from three relevant aquaculture systems and estimate human exposure to MPs and metals through seabass consumption. The recirculating aquaculture system (RAS) had the highest MP occurrence in water and feed. MP levels in seabass followed the aquaculture system's levels in water and feed, with RAS-farmed fish presenting the highest MP load, both in the fish gastrointestinal tract (GIT) and muscle, followed by pond-, and cage-farmed fish. MPs' characteristics across aquaculture systems and fish samples remained consistent, with the predominant recovered particles falling within the MP size range. The particles were visually characterized and chemically identified by micro-Fourier Transform Infrared Spectroscopy (μFTIR). Most of these particles were fibres composed of man-made cellulose and PET. MP levels in GIT were significantly higher than in muscle for pond- and RAS-farmed fish, MPs' bioconcentration factors >1 indicated bioconcentration in farmed seabass. Metal concentrations in fish muscle were below permissible limits, posing low intake risks for consumers according to the available health-based guidance values and estimated dietary scenarios., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
3. Microbial response to a port fuel spill: Community dynamics and potential for bioremediation.
- Author
-
Perdigão R, Tomasino MP, Magalhães C, Carvalho MF, Almeida CMR, and Mucha AP
- Subjects
- Bacteria metabolism, Petroleum metabolism, Portugal, Microbiota, Biodegradation, Environmental, Petroleum Pollution, Water Pollutants, Chemical metabolism, Water Pollutants, Chemical analysis, Seawater microbiology, Hydrocarbons metabolism
- Abstract
Following a fuel leakage inside a Portuguese maritime port, we conducted parallel 30-day experiments using contaminated seawater and fuel, sampled five days after the incident. This study aimed to (i)survey the native microbial community response to the spilled fuel and (ii)evaluate the efficacy of bioremediation, both biostimulation and bioaugmentation with a lyophilized bacterial consortium (Rhodococcus erythropolis, Pseudomonas sp.), in accelerating hydrocarbon degradation. Metabarcoding analysis revealed a shift in microbial communities, with increased abundance of hydrocarbon-degraders (e.g. Alcanivorax, Thalassospira). Ninety-five hydrocarbonoclastic bacteria were isolated, including key groups from the enriched communities. The lyophilized bacteria added in bioaugmentation, enhanced the abundance of hydrocarbon-degraders over time and were recovered throughout time. Bioremediation treatments favoured biodegradation, achieving over 60 % removal of total petroleum hydrocarbons after 15 days, contrasting with natural attenuation where almost no TPH was removed. This work highlights the potential of bioremediation technologies to accelerate hydrocarbon-degrading activity, for oil spills inside ports., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
4. Potential of nature-based solutions to reduce antibiotics, antimicrobial resistance, and pathogens in aquatic ecosystems. a critical review.
- Author
-
Pastor-López EJ, Escolà M, Kisielius V, Arias CA, Carvalho PN, Gorito AM, Ramos S, Freitas V, Guimarães L, Almeida CMR, Müller JA, Küster E, Kilian RM, Diawara A, Ba S, and Matamoros V
- Subjects
- Wetlands, Drug Resistance, Microbial, Waste Disposal, Fluid methods, Water Pollutants, Chemical analysis, Wastewater microbiology, Rivers microbiology, Rivers chemistry, Anti-Bacterial Agents, Ecosystem
- Abstract
This comprehensive scientific review evaluates the effectiveness of nature-based solutions (NBS) in reducing antibiotics (ABs), combating antimicrobial resistance (AMR), and controlling pathogens in various aquatic environments at different river catchment levels. It covers conventional and innovative treatment wetland configurations for wastewater treatment to reduce pollutant discharge into the aquatic ecosystems as well as exploring how river restoration and saltmarshes can enhance pollutant removal. Through the analysis of experimental studies and case examples, the review shows NBS's potential for providing sustainable and cost-effective solutions to improve the health of aquatic ecosystems. It also evaluates the use of diagnostic indicators to predict NBS effectiveness in removing specific pollutants such as ABs and AMR. The review concludes that NBS are feasible for addressing the new challenges stemming from human activities such as the presence of ABs, AMR and pathogens, contributing to a better understanding of NBS, highlighting success stories, addressing knowledge gaps, and providing recommendations for future research and implementation., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
5. Metformin and lamotrigine sorption on a digestate amended soil in presence of trace metal contamination.
- Author
-
Baldasso V, Sayen S, Gomes CAR, Frunzo L, Almeida CMR, and Guillon E
- Subjects
- Soil, Lamotrigine, Anticonvulsants, Adsorption, Pharmaceutical Preparations, Metals, Heavy analysis, Metformin, Soil Pollutants analysis, Trace Elements
- Abstract
The antidiabetic drug metformin and antiepileptic drug lamotrigine are contaminants of emerging concern that have been detected in biowaste-derived amendments and in the environment, and their fate must be carefully studied. This work aimed to evaluate their sorption behaviour on soil upon digestate application. Experiments were conducted on soil and digestate-amended soil as a function of time to study kinetic processes, and at equilibrium also regarding the influence of trace metals (Pb, Ni, Cr, Co, Cu, Zn) at ratio pharmaceutical/metal 1/1, 1/10, and 1/100. Pharmaceutical desorption experiments were also conducted to assess their potential mobility to groundwater. Results revealed that digestate amendment increased metformin and lamotrigine adsorbed amounts by 210% and 240%, respectively, increasing organic matter content. Metformin adsorption kinetics were best described by Langmuir model and those of lamotrigine by Elovich and intraparticle diffusion models. Trace metals did not significantly affect the adsorption of metformin in amended soil while significantly decreased that of lamotrigine by 12-39%, with exception for Cu
2+ that increased both pharmaceuticals adsorbed amounts by 5 - 8%. This study highlighted the influence of digestate amendment on pharmaceutical adsorption and fate in soil, which must be considered in the circular economy scenario of waste-to-resource., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)- Published
- 2024
- Full Text
- View/download PDF
6. Spatial distribution and temporal trends of butyltin compounds (TBT, DBT & MBT) in short sediment cores of the SW Portuguese Shelf (western Iberian Margin, NE Atlantic).
- Author
-
Mil-Homens M, Almeida CMR, Dias S, Soares W, van Gaever P, de Stigter H, Santos MM, Santana A, Freitas M, Abrantes F, and Caetano M
- Abstract
Spatial patterns and temporal trends of the butyltin compounds tributyltin (TBT), dibutyltin (DBT), and monobutyltin (MBT) were investigated in a set of sediment samples collected along the SW Portuguese continental shelf. This region did not reach the Good Environmental Status (GES) in accordance with the Marine Strategy Framework Directive (MSFD) during a first evaluation carried out in 2012. Overall, MBT and DBT were the predominant organotin species detected, but high concentrations of TBT were found in and around disposal sites for dredge sludge derived from the dredging in navigation channels, harbours, and shipyard facilities of the Tagus and Sado estuaries. Although Portuguese regulations for monitoring sediment quality in relation to dredging activities consider only PAH, PCB and HCB, they also dictate that other organic contaminants such as butyltin compounds (BTs) should be monitored if suspicion of high values exists, but no action limits are defined for these (MAOTDR, 2007). Without action limits, the monitoring recommendation given in the regulations is not put into practice. Considering their toxicity, BT derivates should be integrated in the legislation, because they represent an environmental threat in the relocation of dredged material, especially when derived from harbour and shipyards areas. Based on this study, we recommend giving more attention to the amounts and impacts of BTs in sediments at dredged material disposal sites (DMDS) and their surroundings. Or even better, in order to be more efficient, monitoring should be done at the source of the dredged materials and not at the sink. In case it is not done, the monitoring of concentrations of TBT (and other BTs) in sediments and organisms, including imposex studies, at all Portuguese sites for disposal of dredged material receiving slightly to strongly contaminated dredged material must be developed., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationship that could have appeared to influence the work reported in this manuscript., (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
7. Buried and surface oil degradation - Evaluating bioremediation to increase PAHs removal through linear mathematical models.
- Author
-
Stoichev T, Mucha AP, Bernabeu A, and Almeida CMR
- Subjects
- Humans, Biodegradation, Environmental, Sand, Linear Models, Soil Microbiology, Polycyclic Aromatic Hydrocarbons analysis, Petroleum analysis, Soil Pollutants analysis
- Abstract
A bioremediation approach with tide simulation for buried and surface oil degradation was tested for removal of two, three and four rings polycyclic aromatic hydrocarbons (PAHs). Linear models depicted degradation constants of individual PAH as simple additive function of their initial concentrations (C
0 ) in contaminated sand, hydrophobicity, sampling layer and treatment conditions. For all PAHs and treatment conditions, the degradation of oil in buried layers was faster than at the surface. Naturally-occurring microorganisms proved to be efficient for bioremediation of PAHs and were stimulated by fertilizer addition (biostimulation, BS). Bioaugmentation (BA) by addition of a slurry of a native oil-degraders pre-stimulated consortium did not show faster PAH degradation than BS. Degradation was more rapid for PAH present at low C0 and with intermediate hydrophobicity. Bioremediation of beach sand either with surface or buried crude oil is a cost-effective strategy to clean-up different hydrocarbon families, including persistent ones, such as PAHs., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)- Published
- 2023
- Full Text
- View/download PDF
8. Microplastic contamination in large migratory fishes collected in the open Atlantic Ocean.
- Author
-
Pereira R, Rodrigues SM, Silva D, Freitas V, Almeida CMR, and Ramos S
- Subjects
- Animals, Humans, Plastics, Environmental Monitoring, Atlantic Ocean, Fishes, Microplastics, Water Pollutants, Chemical analysis
- Abstract
Fishes are one of the most important components of the oceans and are exposed to several anthropogenic pressures, namely microplastic (MP), contaminants that are now ubiquitous worldwide. Taking advantage of the 2020 Circumnavigation Expedition carried by the NRP Sagres tall ship of the Portuguese Navy, fish samples from the southern Atlantic ocean were collected to evaluate possible MP contamination. In a total of 14 weeks of campaign, seven large migratory fishes of commercial interest were collected across the middle Atlantic Ocean and along the South American Atlantic coast. All individuals were contaminated with MPs, with an average of 18 ± 11 MPs/fish. In all fish sampled, both the gastrointestinal tract and gills presented MPs, indicating different contamination pathways including via their preys and from surrounding water, respectively. A total of 124 MPs were observed, where 72 % were fibers and 28 % particles, mostly of blue color (85 %), and with rayon and nylon as the most abundant polymers. This study is an important contribution to increase the scientific knowledge of MP contamination in mesopelagic fishes used for human consumption and collected in the open waters, reinforcing the need for further research regarding MP contamination in top predatory species from high trophic levels., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
9. Advanced oxidation technologies and constructed wetlands in aquaculture farms: What do we know so far about micropollutant removal?
- Author
-
Gorito AM, Lado Ribeiro AR, Pereira MFR, Almeida CMR, and Silva AMT
- Subjects
- Animals, Aquaculture, Ecosystem, Humans, Prospective Studies, Waste Disposal, Fluid, Wastewater, Water Pollutants, Chemical analysis, Wetlands
- Abstract
Aquaculture is the fastest growing animal food-producing sector. Water is the central resource for aquaculture, and it is essential that its quality be preserved. Micropollutants (MPs) can reach aquaculture through anthropogenic addition or inlet water, and may cause harmful effects such as endocrine disruption and antibiotic resistance, adversely affecting the fish species being farmed. Furthermore, the discharge of aquaculture effluents into the environment may contribute to the deterioration of water courses. In this sense, the implementation of environmentally responsible measures in aquaculture farms is imperative for the protection of ecosystems and human health. The European Commission (EC) has recently launched a guiding document promoting ecological aquaculture practices; however, options for water treatment are still lacking. Conventional processes are not designed to deal with MPs; this review article consolidates relevant information on the application of advanced oxidation technologies (AOTs) and constructed wetlands (CWs) as potential strategies in this regard. Although 161 studies on the application of AOTs or CWs in aquaculture have already been published, only 34 focused on MPs (28 on AOTs and 6 on CWs), whereas the others reported the removal of contaminants such as bacteria, organic matter, solids and inorganic ions. No study coupling both treatments has been reported to date for the removal of MPs from aquaculture waters. AOTs and CWs are prospective alternatives for the treatment of aquacultural aqueous matrices. However, the type of aquaculture activity and the specifications of these available technologies should be considered while selecting the most suitable treatment option., (Copyright © 2021 Elsevier Inc. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
10. Microplastics as a vehicle of exposure to chemical contamination in freshwater systems: Current research status and way forward.
- Author
-
Eder ML, Oliva-Teles L, Pinto R, Carvalho AP, Almeida CMR, Hornek-Gausterer R, and Guimarães L
- Subjects
- Ecosystem, Environmental Monitoring, Fresh Water, Plastics toxicity, Microplastics, Water Pollutants, Chemical analysis, Water Pollutants, Chemical toxicity
- Abstract
Contamination by microplastics is increasing steadily worldwide, affecting all environments. Additionally, aquatic organisms are often exposed to mixtures of other contaminants, including various chemicals. Numerous studies reported adsorption of chemicals to microplastics, raising concern about their possible role as vehicles of exposure through transfer to biota. Nevertheless, until recently, the studies on the topic were mostly focused on the marine environment. In the past five years, however, plenty of publications contributed empirical data about freshwater ecosystems, raising the need for a critical appraisal of the information. Herein the scientific literature was reviewed and multivariate data analysis was done. The analysed studies employed widely different experimental designs, endpoints, test species, shapes and concentrations of various polymer types and chemicals, often not relevant for the freshwater environment. Our integrated analytical approach revealed unfathomable research gaps, given the theoretical knowledge available and lessons learned from research about the marine environment. Greater harmonization of laboratory studies investigating this topic is needed, as well as testing conditions reflecting real exposure scenarios. Furthermore, standardized testing protocols are urgently required to guide such experiments and improve the comparability of the results obtained., (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
11. Microplastics and plankton: Knowledge from laboratory and field studies to distinguish contamination from pollution.
- Author
-
Rodrigues SM, Elliott M, Almeida CMR, and Ramos S
- Subjects
- Ecosystem, Environmental Monitoring, Laboratories, Plankton, Plastics, Microplastics, Water Pollutants, Chemical analysis, Water Pollutants, Chemical toxicity
- Abstract
Due to their ubiquitous presence, size and characteristics as ability to adsorb pollutants, microplastics are hypothesized as causing a major impact on smaller organisms, such as plankton. Despite this, there is a need to determine whether these impacts just relate to the environmental presence of the materials or their effects on biological processes. Therefore, we aimed to 1) review current research on plankton and microplastics; 2) compare field and laboratory experimental findings, and 3) identify knowledge gaps. The systematic review showed that 70% of the 147 relevant scientific publications were from laboratory studies and microplastics interactions with plankton were recorded in 88 taxa. Field study publications were relatively scarce and the characteristics of microplastics collected in the field were very different from those used in laboratory experiments thereby limiting the comparison between studies. Our systematic review highlighted knowledge gaps in: 1) the number of field studies; 2) the non-comparability between laboratory and field conditions, and 3) the low diversity of plankton species studied. Furthermore, this review indicated that while there are many studies on contamination by microplastics, the effects of this contamination (i.e., pollution per se) have been less well-studied, especially in the field at population, community, and ecosystem levels., (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
12. Microbial degradation of two highly persistent fluorinated fungicides - epoxiconazole and fludioxonil.
- Author
-
Alexandrino DAM, Mucha AP, Almeida CMR, and Carvalho MF
- Subjects
- Biodegradation, Environmental, Sodium Acetate metabolism, Soil Microbiology, Water Purification methods, Epoxy Compounds metabolism, Fungicides, Industrial metabolism, Microbial Consortia physiology, Persistent Organic Pollutants metabolism, Triazoles metabolism, Water Pollutants, Chemical metabolism
- Abstract
Biodegradation of two highly persistent fluorinated fungicides, epoxiconazole (EPO) and fludioxonil (FLU), by microbial consortia enriched from estuarine sediment and agricultural soil is reported. After an enrichment period of 6 months, four microbial consortia were able to completely remove and defluorinate the fungicides in co-metabolic conditions. Defluorination was biologically mediated and results suggest it is not a primary catabolic step, as fungicide removal was always faster than its defluorination. Three of the four enriched consortia had similar biodegradation performances in the absence of a co-substrate. Biodegradation kinetics revealed that microbial degradation followed a first-order kinetics, with cultures being capable of biodegrading concentrations up to 10 mg L
-1 of EPO or FLU, in a maximum of 21 days. Estimated half-life values for these compounds were significantly lower than those reported in literature, highlighting the unique metabolic performance of the obtained consortia. Analysis of their microbial composition revealed that they integrate several bacterial species belonging to the Proteobacteria phylum, with the most common genera being Pseudomonas, Ochrobactrum and Comamonas. This is the first study providing clear evidence on the biodegradation of EPO and FLU, opening doors for the design of bioremediation technologies for the recovery of ecosystems polluted with such recalcitrant compounds., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)- Published
- 2020
- Full Text
- View/download PDF
13. Adsorption of Cd and Cu to different types of microplastics in estuarine salt marsh medium.
- Author
-
Almeida CMR, Manjate E, and Ramos S
- Subjects
- Adsorption, Plastics, Cadmium chemistry, Copper chemistry, Microplastics chemistry, Water Pollutants, Chemical, Wetlands
- Abstract
This study aimed to investigate if microplastics (MPs) type (polyethylene microspheres (mPE), fishing line fibers, film plastic bags MPs and bottle cap particles) and aging affect MPs capacity to sorb Cd or Cu in estuarine salt marsh medium. Tests were carried out in elutriate solution, a simple medium obtained by mixing rhizosediment (sediment in contact with plants roots) with the respective estuarine water, that can be used to simulate water-sediment exchanges in estuarine salt marsh environments. After 7 days of exposure, metals adsorption was only detected for film MPs. No differences were observed between virgin and aged MPs. Salinity also did not influence metal adsorption to mPE. Present results indicate that in estuarine salt marsh areas some types of MPs might adsorb metals, which could affect metals availability., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2019 Elsevier Ltd. All rights reserved.)
- Published
- 2020
- Full Text
- View/download PDF
14. Quenchers in advanced oxidation technologies for analysis of micropollutants by liquid chromatography coupled to mass spectrometry: Sodium sulphite or catalase?
- Author
-
Gorito AM, Barbosa MO, Almeida CMR, Pereira MFR, Silva AMT, and Ribeiro ARL
- Abstract
This work aimed to investigate the possible effect of 2 quenchers commonly used in H
2 O2 -based advanced oxidation technologies (AOTs), i.e. catalase and sodium sulphite (Na2 SO3 ), on the analytical signal of 3 detectors coupled to liquid chromatography (LC): tandem mass spectrometry (LC-MS/MS), fluorescence detection (LC-FD) and LC-diode array detection (LC-DAD). The observation of analytical interferences for a group of compounds when studying the removal by continuous mode UV/H2 O2 of 26 micropollutants (MPs) from a spiked surface water (SW), for which the residual H2 O2 in the samples was quenched by Na2 SO3 , triggered the need of understanding these effects and thus catalase was used as comparative quencher. From the 26 MPs having a wide range of polarity and pKa , those monitored after electrospray ionization (ESI) under positive ionization (PI) mode and presenting a pKa higher than 5.9 revealed a great signal suppression, but only when using Na2 SO3 as H2 O2 quencher. In this sense, we further explored this effect by selecting 2 MPs, metoprolol and diclofenac, which had respectively signal suppression and no interference in the LC-MS/MS response. These MPs were analysed before and after addition of H2 O2 and catalase or Na2 SO3 in reaction vials, using: (i) different detectors coupled to LC, namely LC-MS/MS with ESI under PI and negative ionization (NI) modes, LC-FD and LC-DAD; (ii) different environmental matrices (SW, drinking water, wastewater) and ultrapure water; and (iii) different magnitude levels (0.1-10 mg L-1 ). The results demonstrated a remarkable signal suppression in LC-MS/MS analyses under PI mode for those compounds with pKa higher than 5.9, confirming the interfering effect of H2 O2 /Na2 SO3 . To the best of our knowledge, the analytical interference in the LC-MS/MS analysis, after adding Na2 SO3 to quench H2 O2 in AOTs experiments was never reported before and the results presented herein support the recommendation to use catalase instead of Na2 SO3 as quencher in AOTs studies., (Copyright © 2019 Elsevier B.V. All rights reserved.)- Published
- 2019
- Full Text
- View/download PDF
15. Persistent and emerging pollutants assessment on aquaculture oysters (Crassostrea gigas) from NW Portuguese coast (Ria De Aveiro).
- Author
-
Gadelha JR, Rocha AC, Camacho C, Eljarrat E, Peris A, Aminot Y, Readman JW, Boti V, Nannou C, Kapsi M, Albanis T, Rocha F, Machado A, Bordalo A, Valente LMP, Nunes ML, Marques A, and Almeida CMR
- Subjects
- Animals, Aquaculture, Environmental Monitoring, Portugal, Crassostrea metabolism, Environmental Exposure analysis, Food Contamination analysis, Shellfish analysis, Water Pollutants, Chemical analysis
- Abstract
The study aim was to determine a range of relevant persistent and emerging pollutants in oysters produced in an aquaculture facility located in an important production area, to assure their safety for human consumption. Pollutants, including 16 PAHs, 3 butyltins (BTs), 29 flame retardants (FRs, including organophosphate and halogenated FRs), 35 pesticides (including 9 pyrethroid insecticides) and 13 personal care products (PCPs, including musks and UV filters), were determined in oysters' tissues collected during one year in four seasonal sampling surveys. The seasonal environmental pollution on the production site was evaluated by water and sediment analysis. Furthermore, oysters' nutritional quality was also assessed and related with the consumption of healthy seafood, showing that oysters are a rich source of protein with low fat content and with a high quality index all year around. Results showed that most analysed pollutants were not detected either in oyster tissues or in environmental matrixes (water and sediments). The few pollutants detected in oyster tissues, including both regulated and non-legislated pollutants, such as a few PAHs (fluorene, phenanthrene, anthracene, fluoranthene, pyrene and indenopyrene), FRs (TPPO, TDCPP, DCP, BDE-47, BDE-209 and Dec 602) and PCPs (galaxolide, galaxolidone, homosalate and octocrylene), were present at low levels (in the ng/g dw range) and did not represent a significant health risk to humans. The observed seasonal variations related to human activities (e.g. tourism in summer) highlights the need for environmental protection and sustainable resource exploration for safe seafood production., (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Published
- 2019
- Full Text
- View/download PDF
16. Enrofloxacin and copper plant uptake by Phragmites australis from a liquid digestate: Single versus combined application.
- Author
-
Sayen S, Rocha C, Silva C, Vulliet E, Guillon E, and Almeida CMR
- Subjects
- Soil, Biodegradation, Environmental, Copper analysis, Enrofloxacin analysis, Poaceae chemistry, Soil Pollutants analysis, Waste Disposal, Fluid
- Abstract
Application of anaerobically digested sludges and manure (digestates) to agricultural fields reduces the need for fertilizer application, but might also pose environmental risks due to the introduction of various pollutants -including metal trace elements and pharmaceuticals- to amended soils. The simultaneous presence of different contaminants can affect plant uptake, altering the performance of phytoremediation. This work aims to investigate and compare the single and combined effects of Enrofloxacin (Enro) and Cu on their plant uptake from digestate solutions. Plant uptake experiments were carried out by exposing, for 5 days, Phragmites australis to three different concentrations of a liquid digestate doped with Cu and/or Enro. Contaminant concentrations were afterward determined in both plant tissues and digestate solutions. Contaminant speciation in solution and Cu speciation in plant roots were also assessed. Cu plant uptake (mostly in plant roots, Cu concentration varied between 55 and 254 μg·g
-1 ) was observed, especially at low concentration of digestate. At high digestate concentration, Cu uptake decreased due to the formation of Cu-DOC complexes rendering Cu less bioavailable. The presence of the antibiotic slightly further reduced Cu plant uptake due to the formation of Cu-Enro complexes. Plant roots also accumulated Enro, an accumulation also higher for the lowest concentration of digestate (an increase of 40%), and that slightly increased in the presence of Cu (up to 226 μg·g-1 ). However, proportion of free Enro in solution decreased (up to 70%) in the presence of Cu due to complexation. Cu speciation in plant roots confirmed the implication of the complexation in the uptake of Cu and Enro when they are simultaneously present in solution. Thus, the presence of amendments (such as digestates) increasing the DOC content, in addition to the interactions between contaminants, should be taken into account for field crop soils as well as for phytoremediation technologies., (Copyright © 2019 Elsevier B.V. All rights reserved.)- Published
- 2019
- Full Text
- View/download PDF
17. Microplastic contamination in an urban estuary: Abundance and distribution of microplastics and fish larvae in the Douro estuary.
- Author
-
Rodrigues SM, Almeida CMR, Silva D, Cunha J, Antunes C, Freitas V, and Ramos S
- Subjects
- Animals, Ecosystem, Fishes physiology, Larva, Plankton, Portugal, Environmental Monitoring, Estuaries, Plastics analysis, Water Pollutants, Chemical analysis
- Abstract
Estuaries are productive environments used by many fish as nursery grounds. The initial stages of fishes are highly vulnerable to (a)biotic factors, and anthropogenic pressures, influencing fish larvae assemblages along the estuary. Microplastics (MPs < 5 mm) are particularly dangerous to early life stages of fishes because their ingestion can induce gut blockage, limiting food intake or exposing organisms to contamination due to MPs capacity to absorb pollutants. Present work aimed to investigate the contamination of an urban impacted estuary (Douro estuary, NW Portugal) by MPs, and study the abundance and distribution of MPs and fish larvae in this estuary. Monthly sampling surveys were performed from December 2016 to December 2017, in nine stations along the estuary. Sub-surface planktonic horizontal trawls were performed to collect fish larvae and MPs. Planktonic samples were sorted, and fish larvae identified. MPs density was determined using a protocol optimized in our laboratory. A total of 1498 fish larvae belonging to 32 taxa were collected, with a mean density of 11.66 fish larvae 100 m
-3 . During the spring-summer period, it was observed the typical increase in the density and diversity of the larval assemblage. Diversity was generally low, with the high dominance of very few taxa, namely the common goby, Pomatoschistus microps. Different types of MPs were found, namely fibers, soft/hard plastic, colorful/transparent plastic, in a total of 2152 particles, with a mean density of 17.06 MPs 100 m-3 . Hard MPs and fibers were the most predominant types, representing 83% of the total MPs collected. In some months the number of MPs surpassed the number of fish larvae, with an average ratio of 1.0 fish larvae:1.5 MPs. Such results are concerning, highlighting that a higher availability of MPs may facilitate their ingestion by fish and therefore increase possible impacts in these communities., (Copyright © 2018 Elsevier B.V. All rights reserved.)- Published
- 2019
- Full Text
- View/download PDF
18. Bioremediation of bezafibrate and paroxetine by microorganisms from estuarine sediment and activated sludge of an associated wastewater treatment plant.
- Author
-
Duarte P, Almeida CMR, Fernandes JP, Morais D, Lino M, Gomes CR, Carvalho MF, and Mucha AP
- Subjects
- Adsorption, Bezafibrate isolation & purification, Bezafibrate metabolism, Biodegradation, Environmental, Microbiota genetics, Paroxetine isolation & purification, Paroxetine metabolism, Pharmaceutical Preparations isolation & purification, Time Factors, Water Pollutants, Chemical isolation & purification, Estuaries, Geologic Sediments microbiology, Pharmaceutical Preparations metabolism, Sewage microbiology, Waste Disposal, Fluid methods, Water Pollutants, Chemical metabolism, Water Purification methods
- Abstract
The present work aimed to explore the potential of autochthonous microorganisms from an urban estuary and from activated sludge of an associated wastewater treatment plant (WWTP), for biodegradation of an antidepressant drug, paroxetine, and on a cholesterol-lowering agent, bezafibrate. These compounds were chosen as representatives of extensively used pharmaceuticals. Autochthonous microorganisms from the indicated sources were exposed to the target pharmaceuticals (1 mg/L) in co-metabolism with sodium acetate (500 mg/L) along a two-weeks period, for a total of 7 two-weeks periods (here referred as cycles). Exposures were carried out in batch mode, under different incubation conditions (agitation vs. static). Removal of pharmaceuticals was monitored at the end of each cycle, by analysing the culture medium. For paroxetine, fluoride ion release was also followed as an indicator of defluorination of the molecule. The structure of the bacterial communities was analysed by ARISA (Automated rRNA Intergenic Spacer Analysis), at the beginning of the experiment and at the end of the first and the last cycles to identify substantial changes associated with the time of exposure, the incubation conditions and the presence and type of pharmaceuticals. Incubation conditions affected not only the bacterial community structure, but also the biodegradation efficiency. At the beginning of the experiment, removal of target pharmaceuticals was found to be lower under agitation than under static conditions, but at the end of the experiment, results showed high removal of the pharmaceuticals from the culture medium (>97%) under both conditions, mainly by microbiological processes. For paroxetine, adsorption and abiotic processes also had an important influence on its removal, but defluorination only occurred in the presence of microorganisms. These results highlight that autochthonous microorganisms from estuarine sediments and WWTP sludge have high ability to remove the selected pharmaceuticals with relevant implications for the development of new bioremediation tools for environmental restoration., (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Published
- 2019
- Full Text
- View/download PDF
19. Performance of secondary wastewater treatment methods for the removal of contaminants of emerging concern implicated in crop uptake and antibiotic resistance spread: A review.
- Author
-
Krzeminski P, Tomei MC, Karaolia P, Langenhoff A, Almeida CMR, Felis E, Gritten F, Andersen HR, Fernandes T, Manaia CM, Rizzo L, and Fatta-Kassinos D
- Subjects
- Recycling, Waste Disposal, Fluid instrumentation, Wastewater microbiology, Crops, Agricultural metabolism, Drug Resistance, Bacterial, Waste Disposal, Fluid methods, Wastewater analysis
- Abstract
Contaminants of emerging concern (CEC) discharged in effluents of wastewater treatment plants (WWTPs), not specifically designed for their removal, pose serious hazards to human health and ecosystems. Their impact is of particular relevance to wastewater disposal and re-use in agricultural settings due to CEC uptake and accumulation in food crops and consequent diffusion into the food-chain. This is the reason why the chemical CEC discussed in this review have been selected considering, besides recalcitrance, frequency of detection and entity of potential hazards, their relevance for crop uptake. Antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) have been included as microbial CEC because of the potential of secondary wastewater treatment to offer conditions favourable to the survival and proliferation of ARB, and dissemination of ARGs. Given the adverse effects of chemical and microbial CEC, their removal is being considered as an additional design criterion, which highlights the necessity of upgrading conventional WWTPs with more effective technologies. In this review, the performance of currently applied biological treatment methods for secondary treatment is analysed. To this end, technological solutions including conventional activated sludge (CAS), membrane bioreactors (MBRs), moving bed biofilm reactors (MBBRs), and nature-based solutions such as constructed wetlands (CWs) are compared for the achievable removal efficiencies of the selected CEC and their potential of acting as reservoirs of ARB&ARGs. With the aim of giving a picture of real systems, this review focuses on data from full-scale and pilot-scale plants treating real urban wastewater. To achieve an integrated assessment, technologies are compared considering also other relevant evaluation parameters such as investment and management costs, complexity of layout and management, present scale of application and need of a post-treatment. Comparison results allow the definition of design and operation strategies for the implementation of CEC removal in WWTPs, when agricultural reuse of effluents is planned., (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Published
- 2019
- Full Text
- View/download PDF
20. Biodegradation of oxytetracycline and enrofloxacin by autochthonous microbial communities from estuarine sediments.
- Author
-
Harrabi M, Alexandrino DAM, Aloulou F, Elleuch B, Liu B, Jia Z, Almeida CMR, Mucha AP, and Carvalho MF
- Subjects
- Anti-Bacterial Agents metabolism, Bacteria classification, Biodegradation, Environmental, Enrofloxacin, Estuaries, Geologic Sediments microbiology, Portugal, Bacteria metabolism, Fluoroquinolones metabolism, Microbiota, Oxytetracycline metabolism, Water Pollutants, Chemical metabolism
- Abstract
This work investigated the potential of microbial communities native to an estuarine environment to biodegrade enrofloxacin (ENR) and oxytetracycline (OXY). Sediments collected from two sites in the Douro river estuary (Porto, Portugal) were used as inocula for the biodegradation experiments. Experiments were carried out for one month, during which ENR and OXY (1 mg L
-1 ) were supplemented individually or in mixture to the cultures at 10-day intervals. Acetate (400 mg L-1 ) was added to the cultures every 3 days to support microbial growth. A series of experimental controls were established in parallel to determine the influence of abiotic breakdown and adsorption in the removal of the antibiotics. Removal of antibiotics was followed by measuring their concentration in the culture medium. Additionally, next-generation sequencing of the 16S rRNA gene amplicon was employed to understand how microbial communities responded to the presence of the antibiotics. At the end of the biodegradation experiments, microbial cultures derived from the two estuarine sediments were able to remove up to 98% of ENR and over 95% of OXY. The mixture of antibiotics did not affect their removal. ENR was removed mainly by biodegradation, while abiotic mechanisms were found to have a higher influence in the removal of OXY. Both antibiotics adsorbed at different extents to the estuarine sediments used as inocula but exhibited a higher affinity to the sediment with finer texture and higher organic matter content. The presence of ENR and OXY in the culture media influenced the dynamics of the microbial communities, resulting in a lower microbial diversity and richness and in the predominance of bacterial species belonging to the phylum Proteobacteria. Therefore, microbial communities native from estuarine environments have potential to respond to the contamination caused by antibiotics and may be considered for the recovering of impacted environments through bioremediation., (Copyright © 2018 Elsevier B.V. All rights reserved.)- Published
- 2019
- Full Text
- View/download PDF
21. Constructed wetland microcosms for the removal of organic micropollutants from freshwater aquaculture effluents.
- Author
-
Gorito AM, Ribeiro AR, Gomes CR, Almeida CMR, and Silva AMT
- Subjects
- Wastewater chemistry, Aquaculture, Waste Disposal, Fluid methods, Water Pollutants, Chemical analysis, Wetlands
- Abstract
The presence of organic micropollutants (MPs) in the aquatic environment is strongly related to their difficult elimination by conventional water and wastewater treatment processes. Therefore, alternative treatment technologies are required to overcome this problem. In this domain, constructed wetlands (CWs) have gained increasing attention in the last years, mainly due to the low-cost, simple operation/maintenance and environmental friendliness of these systems. However, studies on the application of CWs to remove MPs from freshwater aquaculture effluents are still scarce. In this work, planted (Phragmites australis) vertical subsurface flow CWs, at microcosm scale, were investigated for the removal of MPs found in non-spiked freshwater aquaculture effluents, namely atrazine, isoproturon, perfluorooctanesulfonic acid (PFOS), clarithromycin, erythromycin, fluoxetine, norfluoxetine, and 2-ethylhexyl-4-methoxycinnamate (EHMC). A wider multi-component set of 36 MPs was also studied by adding these MPs at 100 ng L
-1 to the same matrix (alachlor, atrazine, chlorfenvinphos, isoproturon, PFOS, azithromycin, clarithromycin, erythromycin, diclofenac, methiocarb, acetamiprid, clothianidin, thiacloprid, thiamethoxam, EHMC, simazine, atorvastatin, bezafibrate, carbamazepine, cephalexin, ceftiofur, citalopram, clindamycin, clofibric acid, diphenhydramine, enrofloxacin, fluoxetine, ketoprofen, metoprolol, norfluoxetine, ofloxacin, propranolol, tramadol, trimethoprim, venlafaxine, and warfarin). High weekly removal efficiencies (>87%) were observed for all MPs in both non-spiked and spiked experiments, with the exception of EHMC (removal rates between 0 and 86%). These results emphasize the potential of CWs to remove MPs from freshwater aquaculture effluents, but also the need to enhance the performance of these systems for the elimination of some recalcitrant MPs, such as EHMC, which was found at high concentrations in the studied effluents., (Copyright © 2018 Elsevier B.V. All rights reserved.)- Published
- 2018
- Full Text
- View/download PDF
22. Indigenous microbial communities along the NW Portuguese Coast: Potential for hydrocarbons degradation and relation with sediment contamination.
- Author
-
Gouveia V, Almeida CMR, Almeida T, Teixeira C, and Mucha AP
- Subjects
- Biodegradation, Environmental, Estuaries, Feces, Gas Chromatography-Mass Spectrometry, Geologic Sediments chemistry, Hydrocarbons analysis, Metals analysis, Metals metabolism, Microbial Consortia genetics, Portugal, Rivers chemistry, Spectroscopy, Fourier Transform Infrared, Water Pollutants, Chemical analysis, Geologic Sediments microbiology, Hydrocarbons metabolism, Microbial Consortia physiology, Water Pollutants, Chemical metabolism
- Abstract
Hydrocarbon degradation (HD) potential by autochthonous microorganisms in the coastal sediments of the NW area of Portugal (coastal sandy beaches and estuaries of the rivers Minho and Douro) was evaluated, investigating if water and sediment contamination/characteristics influence it. Sediments were characterized for microbial abundance (by DAPI), HD microorganisms' abundance (by MPN), microbial community structure (by ARISA), hydrocarbons (by FTIR and SPME-GC-MS), hazardous and noxious substances (SPME-GC-MS) and metals (by AAS). To our knowledge, this is the first time all these pollutants, including the selected HNS, were measured simultaneously in sediments of the selected coastal area. Higher contaminants concentrations were, generally, registered in Douro samples. A clear differentiation of the microbial community structure between beaches and estuaries was observed, as well as, between Douro and Minho river estuaries. BIO-ENV analysis indicated both sediment characteristics (e.g. OM content) and contaminants presence/concentrations (e.g. tetrachloroethylene presence) affected the structure of the microbial community along the studied areas. In all the selected sites, the characterized autochthonous microbial communities showed potential for hydrocarbons degradation, with HD microorganisms being found in all collected sediments. These microorganisms can be a valuable asset to recover contaminated areas, but sediment characteristics and contaminants presence/levels need to be taken into account as they can affect their bioremediation potential and the success of their application as biotechnological tool., (Copyright © 2018 Elsevier Ltd. All rights reserved.)
- Published
- 2018
- Full Text
- View/download PDF
23. Bacterial community dynamic associated with autochthonous bioaugmentation for enhanced Cu phytoremediation of salt-marsh sediments.
- Author
-
Almeida CMR, Oliveira T, Reis I, Gomes CR, and Mucha AP
- Subjects
- Bacteria classification, Copper metabolism, Estuaries, Geologic Sediments chemistry, Geologic Sediments microbiology, Microbial Consortia, Poaceae, Water Pollutants, Chemical metabolism, Wetlands, Bacteria growth & development, Biodegradation, Environmental, Copper analysis, Water Microbiology, Water Pollutants, Chemical analysis
- Abstract
Autochthonous bioaugmentation for metal phytoremediation is still little explored, particularly its application to estuarine salt marshes, but results obtained so far are promising. Nevertheless, understanding the behaviour of the microbial communities in the process of bioaugmentation and their role in improving metal phytoremediation is very important to fully validate the application of this biological technology. This study aimed to characterize the bacterial community dynamic associated with the application of autochthonous bioaugmentation in an experimentation which showed that Phragmites australis rhizosphere microorganisms could increase this salt marsh plant potential to phytoremediate Cu contaminated sediments. Bacterial communities present in the autochthonous microbial consortium resistant to Cu added to the medium and in the sediment at the beginning and at the end of the experiment were characterized by ARISA. Complementarily, the consortium and the sediment used for its production were characterized by next generation sequencing using the pyrosequencing platform 454. The microbial consortium resistant to Cu obtained from non-vegetated sediment was dominated by the genus Lactococcus (46%), Raoultella (25%), Bacillus (12%) and Acinetobacter (11%), whereas the one obtained form rhizosediment was dominated by the genus Gluconacetobacter (77%), Bacillus (17%) and Dyella (3%). Results clearly showed that, after two months of experiment, Cu caused a shift in the bacterial community structure of sediments, an effect that was observed either with or without addition of the metal resistant microbial consortium. Therefore, bioaugmentation application improved the process of phytoremediation (metal translocation by the plant was increased) without inducing long term changes in the bacterial community structure of the sediments. So, phytoremediation combined with autochthonous bioaugmentation can be a suitable technology for the recovery of estuarine areas, contributing for an efficient risk management strategy of these coastal zones., (Copyright © 2017. Published by Elsevier Ltd.)
- Published
- 2017
- Full Text
- View/download PDF
24. Silver nanoparticles uptake by salt marsh plants - Implications for phytoremediation processes and effects in microbial community dynamics.
- Author
-
Fernandes JP, Mucha AP, Francisco T, Gomes CR, and Almeida CMR
- Subjects
- Nanoparticles, Plant Roots chemistry, Silver pharmacokinetics, Biodegradation, Environmental, Metal Nanoparticles, Poaceae, Wetlands
- Abstract
This study investigated the uptake of silver nanoparticles (AgNPs) by a salt marsh plant, Phragmites australis, as well as AgNPs effects on rhizospheric microbial community, evaluating the implications for phytoremediation processes. Experiments were carried out with elutriate solution doped with Ag, either in ionic form or in NP form. Metal uptake was evaluated in plant tissues, elutriate solutions and sediments (by AAS) and microbial community was characterized in terms of bacterial community structure (evaluated by ARISA). Results showed Ag accumulation but only in plant belowground tissues and only in the absence of rhizosediment, the presence of sediment reducing Ag availability. But in plant roots Ag accumulation was higher when Ag was in NP form. Multivariate analysis of ARISA profiles showed significant effect of the absence/presence of Ag either in ionic or NP form on microbial community structure, although without significant differences among bacterial richness and diversity. Overall, P. australis can be useful for phytoremediation of medium contaminated with Ag, including with AgNPs. However, the presence of Ag in either forms affected the microbial community structure, which may cause disturbances in ecosystems function and compromise phytoremediation processes. Such considerations need to be address regarding environmental management strategies applied to the very important estuarine areas., Capsule: The form in which the metal was added affected metal uptake by Phragmites australis and rhizosediment microbial community structure, which can affect phytoremediation., (Copyright © 2017 Elsevier Ltd. All rights reserved.)
- Published
- 2017
- Full Text
- View/download PDF
25. Biodegradation of the veterinary antibiotics enrofloxacin and ceftiofur and associated microbial community dynamics.
- Author
-
Alexandrino DAM, Mucha AP, Almeida CMR, Gao W, Jia Z, and Carvalho MF
- Subjects
- Biodegradation, Environmental, Enrofloxacin, Environmental Monitoring, Wetlands, Anti-Bacterial Agents analysis, Cephalosporins analysis, Fluoroquinolones analysis, Water Microbiology, Water Pollutants, Chemical analysis
- Abstract
Fluoroquinolones and cephalosporins are two classes of veterinary antibiotics arising as pollutants of emerging concern. In this work, the microbial degradation of two representative antibiotics of both these classes, enrofloxacin (ENR) and ceftiofur (CEF), is reported. Biodegradation of the target antibiotics was investigated by supplementing the culture medium with ENR and CEF, individually and in mixture. Microbial inocula were obtained from rhizosphere sediments of plants derived from experimental constructed wetlands designed for the treatment of livestock wastewaters contaminated with trace amounts of these antibiotics. Selected microbial inocula were acclimated during a period of 5months, where the antibiotics were supplemented every three weeks at the concentration of 1mgL
-1 , using acetate as a co-substrate. After this period, the acclimated consortia were investigated for their capacity to biodegrade 2 and 3mgL-1 of ENR and CEF. Complete removal of CEF from the inoculated culture medium was always observed within 21days, independently of its concentration or the concomitant presence of ENR. Biodegradation of ENR decreased with the increase in its concentration in the culture medium, with defluorination percentages decreasing from ca. 65 to 4%. Ciprofloxacin and norfloxacin were detected as biodegradation intermediates of ENR in the microbial cultures supplemented with this antibiotic, indicating that defluorination of at least part of ENR in these cultures is not an immediate catabolic step. Abiotic mechanisms showed high influence in the removal of CEF, affecting less ENR degradation. The acclimation process with the target antibiotics led to significant shifts in the structure and diversity of the microbial communities, predominantly selecting microorganisms belonging to the phyla Proteobacteria (e.g. Achromobacter, Variovorax and Stenotrophomonas genera) and Bacteroidetes (e.g. Dysgonomonas, Flavobacterium and Chryseobacterium genera). The results presented in this study indicate that biodegradation can be an important mechanism for the environmental removal of the tested compounds., (Copyright © 2016 Elsevier B.V. All rights reserved.)- Published
- 2017
- Full Text
- View/download PDF
26. Response of microbial communities colonizing salt marsh plants rhizosphere to copper oxide nanoparticles contamination and its implications for phytoremediation processes.
- Author
-
Fernandes JP, Almeida CMR, Andreotti F, Barros L, Almeida T, and Mucha AP
- Subjects
- Biodegradation, Environmental, Estuaries, Nanoparticles, Oxides, Poaceae microbiology, Portugal, Copper analysis, Rhizosphere, Soil Microbiology, Soil Pollutants analysis, Wetlands
- Abstract
This study aimed to investigate Cu oxide nanoparticles (CuO NP) effect on microbial communities associated with salt marsh plants (Halimione portulacoides and Pragmites australis) rhizosphere and its implications for phytoremediation processes. Experiments were conducted, under controlled conditions, over one week. Rhizosediment soaked in the respective elutriate (a simplified natural medium) with or without plants, was doped with CuO NP or with Cu in ionic form. Microbial community in rhizosediments was characterized in terms of abundance (by DAPI) and structure (by ARISA). Metal uptake by plants was evaluated by measuring Cu in plant tissues (by atomic absorption spectroscopy). Results revealed significant metal uptake but only in plant roots, which was significantly lower (H. portulacoides) or not significant (P. australis) when the metal was in NP form. Microbial community structure was significantly changed by the treatment (absence/presence of Cu, ionic Cu or CuO NP) as showed by multivariate analysis of ARISA profiles and confirmed by analysis of similarities (Global test - one way ANOSIM). Moreover, in P. australis rhizosediments microbial abundance, bacterial richness and diversity indexes were significantly affected (increased or decreased) due to metal presence whereas in H. portulacoides rhizosediment microbial abundance showed a significant decrease, particularly when the metal was in NP form. Accordingly, Cu presence affected the response of the rhizosphere microbial community and in some cases that response was significantly different when Cu was in NP form. The response of the microbial communities to Cu NP might also contribute to the lower metal accumulation by plants when the metal was in this form. So, Cu NP may cause disturbances in ecosystems functions, ultimately affecting phytoremediation processes. These facts should be considered regarding the use of appropriate salt marshes plants to remediate moderately impacted areas such as estuaries, where NPs can be found., (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Published
- 2017
- Full Text
- View/download PDF
27. Constructed wetlands for the removal of metals from livestock wastewater - Can the presence of veterinary antibiotics affect removals?
- Author
-
Almeida CM, Santos F, Ferreira AC, Gomes CR, Basto MC, and Mucha AP
- Subjects
- Animals, Cephalosporins analysis, Enrofloxacin, Fluoroquinolones analysis, Livestock, Poaceae metabolism, Anti-Bacterial Agents analysis, Metals, Heavy analysis, Veterinary Drugs analysis, Wastewater chemistry, Water Pollutants, Chemical analysis, Wetlands
- Abstract
The presence of emergent antibiotics, in livestock wastewater may affect constructed wetlands (CWs) performance in the removal of other pollutants. The main objective of this study was to evaluate the influence of two antibiotics commonly used in livestock industry, enrofloxacin and ceftiofur, on metal removal by CWs. Microcosms (0.4m×0.3m×0.3m), simulating CWs, were constructed with Phragmites australis to treat livestock wastewater spiked or not with 100µg/L of enrofloxacin or ceftiofur (individually or in mixture). Wastewater was treated during 20 one-week cycles. After one-week cycle wastewater was removed and replaced by new wastewater (with or without spiking). At weeks 1, 2, 4, 8, 14, 18 and 20, treated wastewater was analysed to determine the removal rates of metals (Zn, Cu, Fe and Mn) and of each antibiotic. At weeks 1, 8 and 20 portions of the plant root substrate were collected and metals determined. At the end of the experiment metal levels were also determined in plant tissues. Removal rate of Fe from wastewater was 99%. Removal rates of Cu and Zn were higher than 85% and 89%, respectively, whereas for Mn removal rates up to 75% were obtained. In general, no significant differences were observed through time in the removals of the different metals, indicating that the systems maintained their functionality during the experimental period. Antibiotics did not interfere with the system depuration capacity, in terms of metals removals from wastewater, and ceftiofur even promoted metal uptake by P. australis. Therefore, CWs seem to be a valuable alternative to remove pollutants, including antibiotics and metals, from livestock wastewaters, reducing the risk the release of these wastewaters might pose into the environment, although more research should be conducted with other antibiotics in CWs., (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Published
- 2017
- Full Text
- View/download PDF
28. Response of a salt marsh microbial community to antibiotic contamination.
- Author
-
Fernandes JP, Almeida CM, Basto MC, and Mucha AP
- Subjects
- Anti-Bacterial Agents toxicity, Estuaries, Poaceae, Portugal, Water Pollutants, Chemical toxicity, Anti-Bacterial Agents analysis, Environmental Monitoring, Water Microbiology, Water Pollutants, Chemical analysis, Wetlands
- Abstract
Salt marsh plants and associated microorganisms can have an important role in contaminant removal from estuaries, through bioremediation processes. Nevertheless, the interaction between emerging contaminants, namely antibiotics, and plant-microorganism associations in estuarine environment are still scarcely known. In this vein, the aim of the present study was to evaluate, in controlled conditions, the response of a salt marsh plant-microorganism association to a contamination with a veterinary antibiotic. For that a salt marsh plant (Phragmites australis) and its respective rhizosediment were collected in a temperate estuary (Lima estuary, NW Portugal) and exposed for 7 days to enrofloxacin (ENR) under different nutritional conditions in sediment elutriates. Response was evaluated in terms of ENR removal and changes in microbial community structure (evaluated by ARISA) and abundance (estimated by DAPI). In general, no significant changes were observed in microbial abundance. Changes in bacterial richness and diversity were observed but only in unplanted systems. However, multivariate analysis of ARISA profiles showed significant effect of both the presence of plant and type of treatment on the microbial community structure, with significant differences among all treatment groups. In addition, plants and associated microorganisms presented a potential for antibiotic removal that, although highly dependent on their nutritional status, can be a valuable asset to recover impacted areas such as estuarine ones., (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Published
- 2015
- Full Text
- View/download PDF
29. Interactions between salt marsh plants and Cu nanoparticles - Effects on metal uptake and phytoremediation processes.
- Author
-
Andreotti F, Mucha AP, Caetano C, Rodrigues P, Rocha Gomes C, and Almeida CM
- Subjects
- Biodegradation, Environmental, Geologic Sediments chemistry, Plant Roots chemistry, Poaceae chemistry, Salinity, Water Pollutants, Chemical, Amaranthaceae chemistry, Copper chemistry, Metal Nanoparticles chemistry, Wetlands
- Abstract
The increased use of metallic nanoparticles (NPs) raises the probability of finding NPs in the environment. A lot of information exists already regarding interactions between plants and metals, but information regarding interactions between metallic NPs and plants, including salt marsh plants, is still lacking. This work aimed to study interactions between CuO NPs and the salt marsh plants Halimione portulacoides and Phragmites australis. In addition, the potential of these plants for phytoremediation of Cu NPs was evaluated. Plants were exposed for 8 days to sediment elutriate solution doped either with CuO or with ionic Cu. Afterwards, total metal concentrations were determined in plant tissues. Both plants accumulated Cu in their roots, but this accumulation was 4 to 10 times lower when the metal was added in NP form. For P. australis, metal translocation occurred when the metal was added either in ionic or in NP form, but for H. portulacoides no metal translocation was observed when NPs were added to the medium. Therefore, interactions between plants and NPs differ with the plant species. These facts should be taken in consideration when applying these plants for phytoremediation of contaminated sediments in estuaries, as the environmental management of these very important ecological areas can be affected., (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Published
- 2015
- Full Text
- View/download PDF
30. Antioxidant response of Phragmites australis to Cu and Cd contamination.
- Author
-
Rocha AC, Almeida CM, Basto MC, and Vasconcelos MT
- Subjects
- Biodegradation, Environmental, Cysteine metabolism, Glutathione metabolism, Glutathione Disulfide metabolism, Plant Leaves drug effects, Plant Leaves metabolism, Plant Roots drug effects, Plant Roots metabolism, Poaceae metabolism, Wetlands, Antioxidants metabolism, Cadmium toxicity, Copper toxicity, Poaceae drug effects, Soil Pollutants toxicity
- Abstract
Metals are known to induce oxidative stress in plant cells. Antioxidant thiolic compounds are known to play an important role in plants׳ defence mechanisms against metal toxicity but, regarding salt marsh plants, their role is still very poorly understood. In this work, the involvement of non-protein thiols (NPT), such as cysteine (Cys), reduced glutathione (GSH), oxidised glutathione (GSSG) and total acid-soluble SH compounds (total thiols), in the tolerance mechanisms of the marsh plant Phragmites australis against Cu and Cd toxicity was assessed. Specimens of this plant, freshly harvested in an estuarine salt marsh, were exposed, for 7 days, to rhizosediment soaked with the respective elutriate contaminated with Cu (0, 10 and 100 mg/L) or Cd (0, 1, 10 mg/L). In terms of NPT production, Cu and Cd contamination induced different responses in P. australis. The content of Cys increased in plant tissue after plant exposure to Cu, whereas Cd contamination led to a decrease in GSSG levels. In general, metal contamination did not cause a significant variation on GSH levels. Both metals influenced, to some extent, the production of other thiolic compounds. Despite the accumulation of considerable amounts of Cu and Cd in belowground tissues, no visible toxicity signs were observed. So, antioxidant thiolic compounds were probably involved in the mechanisms used by P. australis to alleviate metal toxicity. As P. australis is considered suitable for phytostabilising metal-contaminated sediments, understanding its tolerance mechanisms to toxic metals is important to optimise the conditions for applying this plant in phytoremediation procedures., (Copyright © 2014 Elsevier Inc. All rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF
31. The effect of sand composition on the degradation of buried oil.
- Author
-
Fernández-Fernández S, Bernabeu AM, Rey D, Mucha AP, Almeida CMR, and Bouchette F
- Subjects
- Bathing Beaches, Carbonates analysis, Environmental Restoration and Remediation, Microscopy, Electron, Scanning, Particle Size, Spain, Geologic Sediments chemistry, Petroleum analysis, Petroleum Pollution statistics & numerical data, Seawater chemistry
- Abstract
The potential effects of the mineralogical composition of sediment on the degradation of oil buried on sandy beaches were investigated. Toward that purpose, a laboratory experiment was carried out with sandy sediment collected along NW Iberian Peninsula beaches, tar-balls from the Prestige oil spill (NW Spain) and seawater. The results indicate that the mineralogical composition is important for the physical appearance of the oil (tar-balls or oil coatings). This finding prompted a reassessment of the current sequence of degradation for buried oil based on compositional factors. Moreover, the halo development of the oil coatings might be enhanced by the carbonate concentration of the sand. These findings open new prospects for future monitoring and management programs for oiled sandy beaches., (Copyright © 2014 Elsevier Ltd. All rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF
32. Development of autochthonous microbial consortia for enhanced phytoremediation of salt-marsh sediments contaminated with cadmium.
- Author
-
Teixeira C, Almeida CM, Nunes da Silva M, Bordalo AA, and Mucha AP
- Subjects
- Biodegradation, Environmental, Geologic Sediments chemistry, Magnoliopsida microbiology, Magnoliopsida physiology, Plant Roots microbiology, Plant Roots physiology, Poaceae microbiology, Poaceae physiology, Cadmium analysis, Geologic Sediments microbiology, Microbial Consortia, Water Pollutants, Chemical analysis, Wetlands
- Abstract
Microbial assisted phytoremediation is a promising, though yet poorly explored, new remediation technique. The aim of this study was to develop autochthonous microbial consortia resistant to cadmium that could enhance phytoremediation of salt-marsh sediments contaminated with this metal. The microbial consortia were selectively enriched from rhizosediments colonized by Juncus maritimus and Phragmites australis. The obtained consortia presented similar microbial abundance but a fairly different community structure, showing that the microbial community was a function of the sediment from which the consortia were enriched. The effect of the bioaugmentation with the developed consortia on cadmium uptake, and the microbial community structure associated to the different sediments were assessed using a microcosm experiment. Our results showed that the addition of the cadmium resistant microbial consortia increased J. maritimus metal phytostabilization capacity. On the other hand, in P. australis, microbial consortia amendment promoted metal phytoextraction. The addition of the consortia did not alter the bacterial structure present in the sediments at the end of the experiments. This study provides new evidences that the development of autochthonous microbial consortia for enhanced phytoremediation of salt-marsh sediments contaminated with cadmium might be a simple, efficient, and environmental friendly remediation procedure., Capsule Abstract: Development of autochthonous microbial consortia resistant to cadmium that enhanced phytoremediation by salt-marsh plants, without a long term effect on sediment bacterial diversity., (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF
33. Potential of bioremediation for buried oil removal in beaches after an oil spill.
- Author
-
Pontes J, Mucha AP, Santos H, Reis I, Bordalo A, Basto MC, Bernabeu A, and Almeida CM
- Subjects
- Biodegradation, Environmental, Seawater, Bathing Beaches, Petroleum Pollution, Water Pollutants, Chemical analysis
- Abstract
Bioremediation potential for buried oil removal, an application still lacking thorough research, was assessed in a specifically designed system in which an artificially contaminated oil layer of sand was buried in a sand column subjected to tidal simulation. The efficiency of biostimulation (BS, fertilizer addition) and bioaugmentation (BA, inoculation of pre-stimulated indigenous hydrocarbon-degrading microorganisms plus fertilizer) compared to natural attenuation was tested during a 180-day experimental period. The effect of BA was evident after 60 days (degradation of hydrocarbons reached 80%). BS efficacy was revealed only after 120 days. Microorganisms and nutrients added at the top of the sand column were able to reach the buried oil layer and contributed to faster oil elimination, an important feature for effective bioremediation treatments. Therefore, autochthonous BA with suitable nutritive conditions results in faster oil-biodegradation, appears to be a cost-effective methodology for buried oil remediation and contributes to the recovery of oil-impacted areas., (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Published
- 2013
- Full Text
- View/download PDF
34. Bacterial community response to petroleum contamination and nutrient addition in sediments from a temperate salt marsh.
- Author
-
Ribeiro H, Mucha AP, Almeida CM, and Bordalo AA
- Subjects
- Analysis of Variance, Biodegradation, Environmental, Cell Count, DNA Primers genetics, Petroleum metabolism, Plant Roots metabolism, Portugal, RNA, Ribosomal genetics, Bacteria drug effects, Biota drug effects, Geologic Sediments analysis, Petroleum toxicity, Plant Roots microbiology, Poaceae metabolism, Wetlands
- Abstract
Microbial communities play an important role in the biodegradation of organic pollutants in sediments, including hydrocarbons. The aim of this study was to evaluate the response of temperate salt marsh microbial communities to petroleum contamination, in terms of community structure, abundance and capacity to degrade hydrocarbons. Sediments un-colonized and colonized (rhizosediments) by Juncus maritimus, Phragmites australis and Triglochin striata were collected in a temperate estuary (Lima, NW Portugal), spiked with petroleum under variable nutritional conditions, and incubated for 15 days. Results showed that plant speciation emerged as the major factor for shaping the rhizosphere community structure, overriding the petroleum influence. Moreover, when exposed to petroleum contamination, the distinct salt marsh microbial communities responded similarly with (i) increased abundance, (ii) changes in structure, and (iii) decreased diversity. Communities, particularly those associated to J. maritimus and P. australis roots displayed a potential to degrade petroleum hydrocarbons, with degradation percentages between 15% and 41%, depending on sediment type and nutritional conditions. In conclusion, distinct salt marsh microbial communities responded similarly to petroleum contamination, but presented different pace, nutritional requirements, and potential for its biodegradation, which should be taken into account when developing bioremediation strategies., (Copyright © 2013 Elsevier B.V. All rights reserved.)
- Published
- 2013
- Full Text
- View/download PDF
35. Influence of a salt marsh plant (Halimione portulacoides) on the concentrations and potential mobility of metals in sediments.
- Author
-
Almeida CM, Mucha AP, Bordalo AA, and Vasconcelos MT
- Subjects
- Amaranthaceae chemistry, Ecosystem, Environmental Monitoring, Geologic Sediments chemistry, Metals, Heavy analysis, Plant Roots metabolism, Portugal, Water Movements, Amaranthaceae metabolism, Geologic Sediments analysis, Metals, Heavy metabolism, Wetlands
- Abstract
Influence of Halimione portulacoides, commonly found in temperate salt marshes, on sediment metal contents, speciation and potential mobility in case of sediment re-suspension was evaluated. Both colonized and non-colonized sediments were studied for total Cd, Cu, Pb and Zn contents and metal fraction exchangeable to water collected in situ. Sediment elutriates, prepared with water collected from each site, were used to simulate a sediment re-suspension phenomenon. As the characteristics and degree of contamination of sediments may influence system behaviour, salt marshes of two Portuguese estuaries, Cavado (NW coast) and Sado (SW coast), were studied. Cu, Pb and Zn contents higher than ERL (quality guideline, effect range-low) were observed, indicating potential risks for living organisms. Strong Cu-complexing organic ligands, also determined in both water and elutriates, were higher in rhizosediment elutriates, at concentrations similar, or even higher, to those of Cu. Such ligands condition metals speciation in the water column and probably also metal bioavailability. From rhizosediment significant amounts of Cu and Zn were transferred to the aqueous phase, concentrations 2-8 times higher than concentrations present in water. In contrast, elutriates of non-colonized sediment removed metals from water, Cu and Zn levels in elutriates being 2-6 times lower than initial ones. Cd and Pb levels in water and elutriates were not measurable in most cases. Results clearly indicate that metals potential solubility in the rhizosphere of plants was markedly higher than that in the surrounding sediment. The obtained results indicated that H. portulacoides presence (and probably other salt marsh plants) may cause a marked increase in metals concentrations in dissolved phase (pore water or even water column if rhizosediment is re-suspended). As salt marsh plants may be abundant in temperate and subtropical estuaries and costal lagoons, this phenomenon should not be disregard in future studies towards the sustainable management of such environments.
- Published
- 2008
- Full Text
- View/download PDF
36. Can PAHs influence Cu accumulation by salt marsh plants?
- Author
-
Almeida CM, Mucha AP, Delgado MF, Caçador MI, Bordalo AA, and Vasconcelos MT
- Subjects
- Adsorption, Amaranthaceae metabolism, Chlorophyll metabolism, Copper analysis, Copper chemistry, Environmental Restoration and Remediation, Geologic Sediments chemistry, Photosynthesis drug effects, Plant Roots drug effects, Plant Roots metabolism, Plant Stems drug effects, Plant Stems metabolism, Water Pollutants, Chemical metabolism, Amaranthaceae drug effects, Copper metabolism, Polycyclic Aromatic Hydrocarbons toxicity, Water Pollutants, Chemical toxicity, Wetlands
- Abstract
The presence of polycyclic aromatic hydrocarbons (PAHs) may change the mechanisms of metal uptake, thus influencing kinetics and extent of metal phytoextraction. Studies on the subject are scarce, particularly for salt marsh plants. The aim of this work was to investigate the effect of PAHs on the uptake of Cu by Halimione portulacoides, a plant commonly found in salt marshes. Experiments were carried out in the laboratory, either in hydroponics (sediment elutriate) or in sediment soaked in elutriate, which were prepared with sediment and water from a salt marsh of the Cavado river estuary (NW Portugal). Groups of H. portulacoides (grown in a greenhouse) were exposed to those media during six days. Cu2+ (as Cu(NO3)2), 10(2) and 10(4) microg l(-1), was added to the media as well as 1.6 microg l(-1) of the sixteen EPA priority PAHs (0.1 microg l(-1) of each PAHs). Cu was assayed in solutions, sediments and different plant tissues before and after experiments. After exposure, photosynthetic efficiency and levels of chlorophylls were also measured, indicating that plant stress indicators were identical in all plants independently of the media to which the plants were exposed. PAHs influenced both the soluble Cu fraction and Cu uptake by plants. The amounts of metal accumulated in both roots and stems were significantly higher when the 10(4) microg l(-1) of Cu enriched elutriate was amended with PAHs. Thus, results suggest that PAHs may modify Cu solubility, the Cu sorption by plants and/or the passive penetration of Cu into the root cells. Therefore, the combined effects of different types of pollutants should be taken in consideration when studying the remediation potential of plants, namely in terms of phytoextraction.
- Published
- 2008
- Full Text
- View/download PDF
37. Variability of metal contents in the sea rush Juncus maritimus-estuarine sediment system through one year of plant's life.
- Author
-
Almeida CM, Mucha AP, and Vasconcelos MT
- Subjects
- Aluminum analysis, Aluminum metabolism, Biomass, Environmental Monitoring, Plant Structures chemistry, Plant Structures physiology, Seasons, Silicon Dioxide, Spectrophotometry, Atomic methods, Time Factors, Water Pollutants, Chemical analysis, Water Pollutants, Chemical metabolism, Geologic Sediments analysis, Magnoliopsida chemistry, Magnoliopsida metabolism, Metals, Heavy analysis, Metals, Heavy metabolism
- Abstract
Metal accumulation by Juncus maritimus and the effect this plant might have on the physico-chemical composition of sediments, from Douro river estuary (NW Portugal), were investigated through one year of plant's life. The contents of Al, Cd, Cr, Cu, Fe, Mn, Ni, Pb and Zn were determined (by atomic absorption spectrophotometry) in sediments, rhizosediments (those among plant roots and rhizomes) and different tissues of J. maritimus. Two estuarine sites with different characteristics were studied, one a sandy sediment with relatively low organic matter content, the other a muddy site. The role of the plant seemed to be markedly dependent on the sediment composition. At the sandy site, significant variations throughout the year (a decrease from winter to summer and an increase in autumn) either in rhizosediment and sediment or in J. maritimus roots were only observed in Cd and Cu levels. These variations were the same in both compartments, probably caused by tidal water movements and the activity of the plant. At the muddy site, variations throughout the year were observed for all studied elements but Pb. Changes in the Cd and Zn contents of roots were opposite to those in the rhizosediment (uptake of Cd and Zn by J. maritimus roots in summer may have caused a decrease in rhizosediment levels). Fe and Mn showed similar patterns of variation in both roots and rhizosediment (contents significantly increasing in summer). Higher contents of Fe and Mn in roots in summer could be related to changes in mass, higher availability of the elements, or accumulation at the surface of the root in (hydr)oxides forms. For the remaining elements no significant correlations between rhizosediment and roots variations were observed, indicating that their uptake and release by roots were not based on single exchanges with the sediment. J. maritimus showed capacity to accumulate Cu, Zn and particularly Cd, thus appearing to be useful for the phytostabilization of these metals.
- Published
- 2006
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.