5 results on '"Aryl diazonium salt"'
Search Results
2. A novel ACE2-Based electrochemical biosensor for sensitive detection of SARS-CoV-2.
- Author
-
Ghaedamini H, Khalaf K, Kim DS, and Tang Y
- Subjects
- Humans, Electrochemical Techniques, Electrodes, Gold chemistry, Angiotensin-Converting Enzyme 2, Biosensing Techniques methods, COVID-19 diagnosis, SARS-CoV-2 isolation & purification
- Abstract
SARS-CoV-2 emerged in late 2019 and quickly spread globally, resulting in significant morbidity, mortality, and socio-economic disruptions. As of now, collaborative global efforts in vaccination and the advent of novel diagnostic tools have considerably curbed the spread and impact of the virus in many regions. Despite this progress, the demand remains for low-cost, accurate, rapid and scalable diagnostic tools to reduce the influence of SARS-CoV-2. Herein, the angiotensin-converting enzyme 2 (ACE2), a receptor for SARS-CoV-2, was immobilized on two types of electrodes, a screen-printed gold electrode (SPGE) and a screen-printed carbon electrode (SPCE), to develop electrochemical biosensors for detecting SARS-CoV-2 with high sensitivity and selectivity. This was achieved by using 1H, 1H, 2H, 2H-perfluorodecanethiol (PFDT) and aryl diazonium salt serving as linkers for SPGEs and SPCEs, respectively. Once SARS-CoV-2 was anchored onto the ACE2, the interaction of the virus with the redox probe was analyzed using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). Aryl diazonium salt was observed as a superior linker compared to PFDT due to its consistent performance in the modification of the SPCEs and effective ACE2 enzyme immobilization. A distinct pair of redox peaks in the cyclic voltammogram of the biosensor modified with aryl diazonium salt highlighted the redox reaction between the functional groups of SARS-CoV-2 and the redox probe. The sensor presented a linear relationship between the redox response and the logarithm of SARS-CoV-2 concentration, with a detection limit of 1.02 × 10
6 TCID50/mL (50% tissue culture infectious dose). Furthermore, the biosensor showed remarkable selectivity towards SARS-CoV-2 over H1N1virus., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Published by Elsevier Inc.)- Published
- 2024
- Full Text
- View/download PDF
3. An electrochemical immunosensor for brain natriuretic peptide prepared with screen-printed carbon electrodes nanostructured with gold nanoparticles grafted through aryl diazonium salt chemistry
- Author
-
Ministerio de Economía y Competitividad (España), Comunidad de Madrid, European Commission, Serafín, Verónica, Torrente-Rodríguez, Rebeca M., González-Cortés, Araceli, García de Frutos, Pablo, Sabaté, Manel, Campuzano, Susana, Yáñez-Sedeño, Pilar, Pingarrón, José Manuel, Ministerio de Economía y Competitividad (España), Comunidad de Madrid, European Commission, Serafín, Verónica, Torrente-Rodríguez, Rebeca M., González-Cortés, Araceli, García de Frutos, Pablo, Sabaté, Manel, Campuzano, Susana, Yáñez-Sedeño, Pilar, and Pingarrón, José Manuel
- Abstract
A sensitive amperometric immunosensor has been prepared by immobilization of capture antibodies onto gold nanoparticles (AuNPs) grafted on a screen-printed carbon electrode (SPCE) through aryl diazonium salt chemistry using 4-aminothiophenol (AuNPs-S-Phe-SPCE). The immunosensor was designed for the accurate determination of clinically relevant levels of B-type natriuretic peptide (BNP) in human serum samples. The nanostructured electrochemical platform resulted in an ordered layer of AuNPs onto SPCEs which combined the advantages of high conductivity and improved stability of immobilized biomolecules. The resulting disposable immunosensor used a sandwich type immunoassay involving a peroxidase-labeled detector antibody. The amperometric transduction was carried out at −0.20 V (vs the Ag pseudo-reference electrode) upon the addition of hydroquinone (HQ) as electron transfer mediator and H2O2 as the enzyme substrate. The nanostructured immunosensors show a storage stability of at least 25 days, a linear range between 0.014 and 15 ng mL−1, and a LOD of 4 pg mL−1, which is 100 times lower than the established cut-off value for heart failure (HF) diagnosis. The performance of the immunosensor is advantageously compared with that provided with immunosensors prepared by grafting SPCE with p-phenylendiamine (H2N-Phe-SPCE) and attaching AuNPs by immersion into an AuNPs suspension or by electrochemical deposition, as well as with immunosensors constructed using commercial AuNPs-modified SPCEs. The developed immunosensor was applied to the successful analysis of human serum from heart failure (HF) patients upon just a 10-times dilution as sample treatment.
- Published
- 2018
4. Electrochemical immunosensor for the determination of the cytokine interferon gamma (IFN-γ) in saliva.
- Author
-
Sánchez-Tirado E, González-Cortés A, Yáñez-Sedeño P, and Pingarrón JM
- Subjects
- Humans, Limit of Detection, Saliva chemistry, Biosensing Techniques methods, Electrochemical Techniques methods, Electrodes, Immunoassay methods, Interferon-gamma analysis, Saliva metabolism
- Abstract
A simple, fast and sensitive amperometric immunosensing method for the determination of the clinically relevant cytokine interferon gamma (IFN-γ) in saliva complying the requirements demanded for this kind of sample is reported. The target analyte was sandwiched between a specific capture antibody covalently immobilized on a screen-printed electrode functionalized by the diazonium salt grafting of p-aminobenzoic acid, and a biotinylated detector antibody labeled with a streptavidin-horseradish peroxidase conjugate. The amperometric responses measured at - 0.20 V vs Ag pseudo-reference electrode upon addition of hydrogen peroxide in the presence of hydroquinone as the redox mediator allowed a calibration plot with a linear range between 2.5 and 2000 pg mL
-1 and a low limit of detection (1.6 pg mL-1 ) to be obtained. In addition, a good selectivity against other non-target proteins was achieved. The developed method was validated by analyzing a WHO 1st International Standard for IFN-γ. In addition, the immunosensor was used for the determination of the endogenous IFN-γ in saliva with results in excellent agreement with those obtained by a commercial ELISA kit., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)- Published
- 2020
- Full Text
- View/download PDF
5. An electrochemical immunosensor for brain natriuretic peptide prepared with screen-printed carbon electrodes nanostructured with gold nanoparticles grafted through aryl diazonium salt chemistry.
- Author
-
Serafín V, Torrente-Rodríguez RM, González-Cortés A, García de Frutos P, Sabaté M, Campuzano S, Yáñez-Sedeño P, and Pingarrón JM
- Subjects
- Aniline Compounds chemistry, Antibodies chemistry, Biomarkers blood, Carbon chemistry, Diazonium Compounds chemistry, Electrodes, Gold chemistry, Heart Failure blood, Humans, Hydrogen Peroxide chemistry, Hydroquinones chemistry, Immunoconjugates chemistry, Metal Nanoparticles ultrastructure, Nanostructures chemistry, Nanostructures ultrastructure, Peroxidase chemistry, Sulfhydryl Compounds chemistry, Biosensing Techniques, Electrochemical Techniques, Heart Failure diagnosis, Immunoassay, Metal Nanoparticles chemistry, Natriuretic Peptide, Brain blood
- Abstract
A sensitive amperometric immunosensor has been prepared by immobilization of capture antibodies onto gold nanoparticles (AuNPs) grafted on a screen-printed carbon electrode (SPCE) through aryl diazonium salt chemistry using 4-aminothiophenol (AuNPs-S-Phe-SPCE). The immunosensor was designed for the accurate determination of clinically relevant levels of B-type natriuretic peptide (BNP) in human serum samples. The nanostructured electrochemical platform resulted in an ordered layer of AuNPs onto SPCEs which combined the advantages of high conductivity and improved stability of immobilized biomolecules. The resulting disposable immunosensor used a sandwich type immunoassay involving a peroxidase-labeled detector antibody. The amperometric transduction was carried out at -0.20V (vs the Ag pseudo-reference electrode) upon the addition of hydroquinone (HQ) as electron transfer mediator and H
2 O2 as the enzyme substrate. The nanostructured immunosensors show a storage stability of at least 25 days, a linear range between 0.014 and 15ngmL-1 , and a LOD of 4pgmL-1 , which is 100 times lower than the established cut-off value for heart failure (HF) diagnosis. The performance of the immunosensor is advantageously compared with that provided with immunosensors prepared by grafting SPCE with p-phenylendiamine (H2 N-Phe-SPCE) and attaching AuNPs by immersion into an AuNPs suspension or by electrochemical deposition, as well as with immunosensors constructed using commercial AuNPs-modified SPCEs. The developed immunosensor was applied to the successful analysis of human serum from heart failure (HF) patients upon just a 10-times dilution as sample treatment., (Copyright © 2017 Elsevier B.V. All rights reserved.)- Published
- 2018
- Full Text
- View/download PDF
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