1. SARS-CoV-2-specific ELISA development.
- Author
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Roy V, Fischinger S, Atyeo C, Slein M, Loos C, Balazs A, Luedemann C, Astudillo MG, Yang D, Wesemann DR, Charles R, Lafrate AJ, Feldman J, Hauser B, Caradonna T, Miller TE, Murali MR, Baden L, Nilles E, Ryan E, Lauffenburger D, Beltran WG, and Alter G
- Subjects
- Antibodies, Viral blood, Antibodies, Viral immunology, Antibodies, Viral isolation & purification, Betacoronavirus immunology, COVID-19, COVID-19 Testing, Coronavirus Infections blood, Coronavirus Infections immunology, Coronavirus Infections virology, Feasibility Studies, High-Throughput Screening Assays, Humans, Pandemics, Pneumonia, Viral blood, Pneumonia, Viral immunology, Pneumonia, Viral virology, Reproducibility of Results, SARS-CoV-2, Sensitivity and Specificity, Time Factors, Betacoronavirus isolation & purification, Clinical Laboratory Techniques methods, Coronavirus Infections diagnosis, Enzyme-Linked Immunosorbent Assay, Pneumonia, Viral diagnosis
- Abstract
Critical to managing the spread of COVID-19 is the ability to diagnose infection and define the acquired immune response across the population. While genomic tests for the novel Several Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) detect the presence of viral RNA for a limited time frame, when the virus is shed in the upper respiratory tract, tests able to define exposure and infection beyond this short window of detectable viral replication are urgently needed. Following infection, antibodies are generated within days, providing a durable read-out and archive of exposure and infection. Several antibody tests have emerged to diagnose SARS-CoV-2. Here we report on a qualified quantitative ELISA assay that displays all the necessary characteristics for high-throughput sample analysis. Collectively, this test offers a quantitative opportunity to define both exposure and levels of immunity to SARS-CoV-2., Competing Interests: Declaration of Competing Interest Galit Alter is a founder of SeromYx., (Copyright © 2020. Published by Elsevier B.V.)
- Published
- 2020
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