10 results on '"Blanco, Gerardo"'
Search Results
2. List of contributors
- Author
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Afonso, João, primary, Almeida, Nuno, additional, Andrade, Patrícia, additional, Blanco, Gerardo, additional, Cardoso, Hélder, additional, Coelho-Prabhu, Nayantara, additional, Cortegoso Valdivia, Pablo, additional, Cotter, José, additional, Delgado, Pilar Esteban, additional, Dias, Emanuel, additional, Dray, Xavier, additional, Fernández-Urien, Ignacio, additional, Ferreira, João, additional, Figueiredo, Pedro, additional, Garrido, Isabel, additional, Gilabert, Pere, additional, Gomes, Catarina, additional, He, Chen, additional, Histace, Aymeric, additional, Jiang, Bin, additional, Jiang, Xi, additional, Laiz, Pablo, additional, Leighton, Jonathan, additional, Liao, Zhuan, additional, Lopes, Susana, additional, Macedo, Guilherme, additional, Martínez, Enrique Pérez-Cuadrado, additional, Martins, Miguel, additional, Mascarenhas, Miguel, additional, Medas, Renato, additional, Mendes, Francisco, additional, Mondragon, Oscar, additional, Pan, Jun, additional, Pasha, Shabana F., additional, Pennazio, Marco, additional, Pinho, Rolando, additional, Qian, Yang-Yang, additional, Ribeiro, Tiago, additional, Robertson, Alexander, additional, Rosa, Bruno, additional, Santos, André, additional, Saraiva, Miguel Mascarenhas, additional, Saurin, Jean-Christophe, additional, Segui, Santi, additional, Silva, Vítor Macedo, additional, Solórzano, Omar, additional, Trindade, Eunice, additional, and Wang, Qiwen, additional
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- 2023
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3. Single-cell Analysis of Debrided Diabetic Foot Ulcers Reveals Dysregulated Wound Healing Environment in non-Hispanic Blacks.
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Choi D, Bakhtiari M, Pilcher W, Huang C, Thomas BE, Mumme H, Blanco G, Rajani R, Schechter MC, Fayfman M, Santamarina G, Bhasin S, and Bhasin M
- Abstract
Diabetic foot ulcer (DFU) is a critical complication of diabetes, but the wound microenvironment and its healing process are not completely understood. In this study, we optimized single-cell profiling from sharp debrided ulcers. Our findings demonstrate that healing-DFUs were significantly enriched with distinct fibroblasts expressing genes related to inflammation (CHI3L1, IL6) and extracellular matrix remodeling (ASPN), validating our previous studies on surgically resected ulcers. The race-focused analysis depicted lower expression of key healing-associated genes such as CHIL3L1, MMP11, and SFRP4 in fibroblasts of non-Hispanic Black (NHB) patients compared to White patients. In cellular communication analysis, healing enriched fibroblasts of NHBs exhibited upregulation of signaling pathways such as WNT while those of White showed IGF and MK pathways upregulation. Our findings advocate race as a risk marker of DFU outcomes, likely reflecting underlying disparities in environmental exposures and access to care that profoundly influence healing markers. Using sharp debrided tissues for single-cell assays, this study highlights the need for in-depth investigations into dysregulated wound healing microenvironments of under-represented racial groups., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
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- 2024
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4. Alternative forms of portal vein revascularization in liver transplant recipients with complex portal vein thrombosis.
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Fundora Y, Hessheimer AJ, Del Prete L, Maroni L, Lanari J, Barrios O, Clarysse M, Gastaca M, Barrera Gómez M, Bonadona A, Janek J, Boscà A, Álamo Martínez JM, Zozaya G, López Garnica D, Magistri P, León F, Magini G, Patrono D, Ničovský J, Hakeem AR, Nadalin S, McCormack L, Palacios P, Zieniewicz K, Blanco G, Nuño J, Pérez Saborido B, Echeverri J, Bynon JS, Martins PN, López López V, Dayangac M, Lodge JPA, Romagnoli R, Toso C, Santoyo J, Di Benedetto F, Gómez-Gavara C, Rotellar F, Gómez-Bravo MÁ, López Andújar R, Girard E, Valdivieso A, Pirenne J, Lladó L, Germani G, Cescon M, Hashimoto K, Quintini C, Cillo U, Polak WG, and Fondevila C
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- Humans, Middle Aged, Portal Vein surgery, Ascites complications, Gastrointestinal Hemorrhage, Severity of Illness Index, Liver Transplantation methods, End Stage Liver Disease complications, Esophageal and Gastric Varices complications, Hypertension, Portal complications, Hypertension, Portal surgery, Venous Thrombosis etiology, Venous Thrombosis surgery
- Abstract
Background & Aims: Complex portal vein thrombosis (PVT) is a challenge in liver transplantation (LT). Extra-anatomical approaches to portal revascularization, including renoportal (RPA), left gastric vein (LGA), pericholedochal vein (PCA), and cavoportal (CPA) anastomoses, have been described in case reports and series. The RP4LT Collaborative was created to record cases of alternative portal revascularization performed for complex PVT., Methods: An international, observational web registry was launched in 2020. Cases of complex PVT undergoing first LT performed with RPA, LGA, PCA, or CPA were recorded and updated through 12/2021., Results: A total of 140 cases were available for analysis: 74 RPA, 18 LGA, 20 PCA, and 28 CPA. Transplants were primarily performed with whole livers (98%) in recipients with median (IQR) age 58 (49-63) years, model for end-stage liver disease score 17 (14-24), and cold ischemia 431 (360-505) minutes. Post-operatively, 49% of recipients developed acute kidney injury, 16% diuretic-responsive ascites, 9% refractory ascites (29% with CPA, p <0.001), and 10% variceal hemorrhage (25% with CPA, p = 0.002). After a median follow-up of 22 (4-67) months, patient and graft 1-/3-/5-year survival rates were 71/67/61% and 69/63/57%, respectively. On multivariate Cox proportional hazards analysis, the only factor significantly and independently associated with all-cause graft loss was non-physiological portal vein reconstruction in which all graft portal inflow arose from recipient systemic circulation (hazard ratio 6.639, 95% CI 2.159-20.422, p = 0.001)., Conclusions: Alternative forms of portal vein anastomosis achieving physiological portal inflow (i.e., at least some recipient splanchnic blood flow reaching transplant graft) offer acceptable post-transplant results in LT candidates with complex PVT. On the contrary, non-physiological portal vein anastomoses fail to resolve portal hypertension and should not be performed., Impact and Implications: Complex portal vein thrombosis (PVT) is a challenge in liver transplantation. Results of this international, multicenter analysis may be used to guide clinical decisions in transplant candidates with complex PVT. Extra-anatomical portal vein anastomoses that allow for at least some recipient splanchnic blood flow to the transplant allograft offer acceptable results. On the other hand, anastomoses that deliver only systemic blood flow to the allograft fail to resolve portal hypertension and should not be performed., (Copyright © 2023 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.)
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- 2023
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5. Abdominal normothermic regional perfusion in controlled donation after circulatory determination of death liver transplantation: Outcomes and risk factors for graft loss.
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Hessheimer AJ, de la Rosa G, Gastaca M, Ruíz P, Otero A, Gómez M, Alconchel F, Ramírez P, Bosca A, López-Andújar R, Atutxa L, Royo-Villanova M, Sánchez B, Santoyo J, Marín LM, Gómez-Bravo MÁ, Mosteiro F, Villegas Herrera MT, Villar Del Moral J, González-Abos C, Vidal B, López-Domínguez J, Lladó L, Roldán J, Justo I, Jiménez C, López-Monclús J, Sánchez-Turrión V, Rodríguez-Laíz G, Velasco Sánchez E, López-Baena JÁ, Caralt M, Charco R, Tomé S, Varo E, Martí-Cruchaga P, Rotellar F, Varona MA, Barrera M, Rodríguez-Sanjuan JC, Briceño J, López D, Blanco G, Nuño J, Pacheco D, Coll E, Domínguez-Gil B, and Fondevila C
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- Aged, Death, Graft Survival, Humans, Middle Aged, Organ Preservation methods, Perfusion methods, Retrospective Studies, Risk Factors, Tissue Donors, Liver Transplantation adverse effects, Liver Transplantation methods, Tissue and Organ Procurement
- Abstract
Postmortem normothermic regional perfusion (NRP) is a rising preservation strategy in controlled donation after circulatory determination of death (cDCD). Herein, we present results for cDCD liver transplants performed in Spain 2012-2019, with outcomes evaluated through December 31, 2020. Results were analyzed retrospectively and according to recovery technique (abdominal NRP [A-NRP] or standard rapid recovery [SRR]). During the study period, 545 cDCD liver transplants were performed with A-NRP and 258 with SRR. Median donor age was 59 years (interquartile range 49-67 years). Adjusted risk estimates were improved with A-NRP for overall biliary complications (OR 0.300, 95% CI 0.197-0.459, p < .001), ischemic type biliary lesions (OR 0.112, 95% CI 0.042-0.299, p < .001), graft loss (HR 0.371, 95% CI 0.267-0.516, p < .001), and patient death (HR 0.540, 95% CI 0.373-0.781, p = .001). Cold ischemia time (HR 1.004, 95% CI 1.001-1.007, p = .021) and re-transplantation indication (HR 9.552, 95% CI 3.519-25.930, p < .001) were significant independent predictors for graft loss among cDCD livers with A-NRP. While use of A-NRP helps overcome traditional limitations in cDCD liver transplantation, opportunity for improvement remains for cases with prolonged cold ischemia and/or technically complex recipients, indicating a potential role for complimentary ex situ perfusion preservation techniques., (© 2021 The American Society of Transplantation and the American Society of Transplant Surgeons.)
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- 2022
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6. "Love is calling": Academic friendship and international research collaboration amid a global pandemic.
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Metcalfe AS and Blanco GL
- Abstract
In this intervention we desire to document and celebrate our own international research collaboration as an intimate long-distance relationship that sustains us amid a global pandemic of the coronavirus that causes COVID-19. We share "love letter" poems that we wrote to each other, in response to a poem by Yayoi Kusama titled "Residing in a Castle of Shed Tears," incorporated into her mirror room installation "Love is Calling." In our discussion we reflect upon the emotional connections that sustain academic researchers, particularly those relationships that extend beyond national boundaries and conventional heteronormative expectations., (© 2021 Elsevier Ltd. All rights reserved.)
- Published
- 2021
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7. The melatonin receptor antagonist luzindole induces Ca 2+ mobilization, reactive oxygen species generation and impairs trypsin secretion in mouse pancreatic acinar cells.
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Estaras M, Ameur FZ, Roncero V, Fernandez-Bermejo M, Blanco G, Lopez D, Mateos JM, Salido GM, and Gonzalez A
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- Acinar Cells cytology, Animals, Calcium metabolism, Male, Mice, Pancreas, Exocrine cytology, Receptors, Melatonin metabolism, Acinar Cells metabolism, Calcium Signaling drug effects, Pancreas, Exocrine metabolism, Reactive Oxygen Species metabolism, Receptors, Melatonin antagonists & inhibitors, Trypsin metabolism, Tryptamines pharmacology
- Abstract
Background: In this work we studied the effects of the melatonin receptor-antagonist luzindole (1 μM-50 μM) on isolated mouse pancreatic acinar cells., Methods: Changes in intracellular free-Ca
2+ concentration, reactive oxygen species production and trypsin secretion were analyzed., Results: Luzindole induced increases in [Ca2+ ]i that diminished CCK-8 induced Ca2+ mobilization, compared with that observed when CCK-8 was applied alone. Treatment of cells with thapsigargin (1 μM), in the absence of Ca2+ in the extracellular medium, evoked a transient increase in [Ca2+ ]i . The additional incubation of cells with luzindole (10 μM) failed to induce further mobilization of Ca2+ . In the presence of luzindole a concentration-dependent increase in ROS generation was observed that decreased in the absence of Ca2+ or by pretreatment of cells with melatonin (100 μM). Incubation of pancreatic acinar cells with luzindole (10 μM) impaired CCK-8-induced trypsin secretion. Melatonin was unable to revert the effect of luzindole on CCK-8-induced trypsin secretion., Conclusion: The melatonin receptor-inhibitor luzindole induces Ca2+ -mediated pro-oxidative conditions and impairment of enzyme secretion, which creates a situation in pancreatic acinar cells that might compromise their function., General Significance: The effects of luzindole that we have observed, might be unspecific and could mislead the observations when it is used to study the actions of melatonin on the gland. Another possibility is that melatonin receptors exhibit a basal or agonist-independent activity in pancreatic acinar cells, which might be modulated by melatonin or luzindole., (Copyright © 2019 Elsevier B.V. All rights reserved.)- Published
- 2019
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8. Reply to: "Normothermic regional perfusion - What is the benefit?"
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Hessheimer AJ, Coll E, Ruíz P, Gastaca M, Rivas JI, Gómez M, Sánchez B, Santoyo J, Ramírez P, Parrilla P, Marín LM, Gómez-Bravo MÁ, García-Valdecasas JC, López-Monclús J, Boscá A, López-Andújar R, Fundora-Suárez Y, Villar J, García-Sesma Á, Jiménez C, Rodríguez-Laíz G, Lladó L, Rodríguez JC, Barrera M, Charco R, López-Baena JÁ, Briceño J, Pardo F, Blanco G, Pacheco D, Domínguez-Gil B, Sánchez Turrión V, and Fondevila C
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- Death, Humans, Organ Preservation, Perfusion, Liver Transplantation
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- 2019
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9. The UK DCD Risk Score: Still no consensus on futility in DCD liver transplantation.
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Hessheimer AJ, Coll E, Ruíz P, Gastaca M, Rivas JI, Gómez M, Sánchez B, Santoyo J, Ramírez P, Parrilla P, Marín LM, Gómez-Bravo MÁ, García-Valdecasas JC, López-Monclús J, Boscá A, López-Andújar R, Fundora-Suárez J, Villar J, García-Sesma Á, Jiménez C, Rodríguez-Laíz G, Lladó L, Rodríguez JC, Barrera M, Charco R, López-Baena JÁ, Briceño J, Pardo F, Blanco G, Pacheco D, Domínguez-Gil B, Sánchez Turrión V, and Fondevila C
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- Death, Humans, Medical Futility, United Kingdom, Liver Transplantation, Tissue and Organ Procurement
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- 2019
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10. Normothermic regional perfusion vs. super-rapid recovery in controlled donation after circulatory death liver transplantation.
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Hessheimer AJ, Coll E, Torres F, Ruíz P, Gastaca M, Rivas JI, Gómez M, Sánchez B, Santoyo J, Ramírez P, Parrilla P, Marín LM, Gómez-Bravo MÁ, García-Valdecasas JC, López-Monclús J, Boscá A, López-Andújar R, Fundora-Suárez J, Villar J, García-Sesma Á, Jiménez C, Rodríguez-Laíz G, Lladó L, Rodríguez JC, Barrera M, Charco R, López-Baena JÁ, Briceño J, Pardo F, Blanco G, Pacheco D, Domínguez-Gil B, Sánchez Turrión V, and Fondevila C
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- Adult, Aged, Cadaver, Female, Follow-Up Studies, Graft Rejection, Humans, Male, Middle Aged, Postoperative Complications, Propensity Score, Spain, Graft Survival, Heart Arrest physiopathology, Liver Transplantation methods, Organ Preservation methods, Perfusion methods, Shock physiopathology, Tissue Donors
- Abstract
Background & Aims: Although there is increasing interest in its use, definitive evidence demonstrating a benefit for postmortem normothermic regional perfusion (NRP) in controlled donation after circulatory death (cDCD) liver transplantation is lacking. The aim of this study was to compare results of cDCD liver transplants performed with postmortem NRP vs. super-rapid recovery (SRR), the current standard for cDCD., Methods: This was an observational cohort study including all cDCD liver transplants performed in Spain between June 2012 and December 2016, with follow-up ending in December 2017. Each donor hospital determined whether organ recovery was performed using NRP or SRR. The propensity scores technique based on the inverse probability of treatment weighting (IPTW) was used to balance covariates across study groups; logistic and Cox regression models were used for binary and time-to-event outcomes., Results: During the study period, there were 95 cDCD liver transplants performed with postmortem NRP and 117 with SRR. The median donor age was 56 years (interquartile range 45-65 years). After IPTW analysis, baseline covariates were balanced, with all absolute standardised differences <0.15. IPTW-adjusted risks were significantly improved among NRP livers for overall biliary complications (odds ratio 0.14; 95% CI 0.06-0.35, p <0.001), ischaemic type biliary lesions (odds ratio 0.11; 95% CI 0.02-0.57; p = 0.008), and graft loss (hazard ratio 0.39; 95% CI 0.20-0.78; p = 0.008)., Conclusions: The use of postmortem NRP in cDCD liver transplantation appears to reduce postoperative biliary complications, ischaemic type biliary lesions and graft loss, and allows for the transplantation of livers even from cDCD donors of advanced age., Lay Summary: This is a propensity-matched nationwide observational cohort study performed using livers recovered from donors undergoing cardiac arrest provoked by the intentional withdrawal of life support (controlled donation after circulatory death, cDCD). Approximately half of the livers were recovered after a period of postmortem in situ normothermic regional perfusion, which restored warm oxygenated blood to the abdominal organs, whereas the remainder were recovered after rapid preservation with a cold solution. The study results suggest that the use of postmortem normothermic regional perfusion helps reduce rates of post-transplant biliary complications and graft loss and allows for the successful transplantation of livers from older cDCD donors., (Copyright © 2018 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.)
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- 2019
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